Lays down RNA Primer

(in leading and lagging)

• Prok: DnaG
• Euk: Pol α

Hooks onto RNA Primer and starts replication

(leading)

• Prok: Pol III
• Euk: Pol δ

Hooks onto RNA Primers and initiates replication

(lagging strand)

• Prok: Pol III
• Euk: Pol α

Synthesizes most of the Okazaki fragment

• Prok: Pol III
• Euk: Pol δ

Finish Okazaki fragment, chew through next RNA primer, and fill gap with DNA

• Prok: Pol I
• Euk: Pol δ

Base excision repair

• Prok: Pol I
• Euk: Pol β

Binds to ssDNA to protect it / prevent it from re-annealing to itself

• In prok: SSB
• In euk: RPA (replication protein A)