Term
| How is karyotype G-banding performed? |
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Definition
| Trypsin followed by Giemsa stain |
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Term
| What is a derivative chromosome? |
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Definition
| Chromosome with an attachement of genetic material of unknown origin |
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Term
| What does FISH allow you to detect? |
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Definition
| the Number and Location of the specific DNA sequence within cells/tissues (both Sensitive and Specific) |
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Term
| What is the smallest size that can be detected with FISH? |
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Definition
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Term
| What are the steps to perform FISH? |
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Definition
| Denature the DNA, Add probe complementry to the desired sequence, visualize |
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Term
| What are the two types of probes used in FISH in Oncology? |
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Definition
| Dual color Dual fision and Dual Color break-apart |
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Term
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Definition
| count chromosomes by binding to centromere |
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Term
| What are the two most common probes used? |
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Definition
| Locus Specific Indicators (LSI, almost all use this) and Centromere Enumeration Probe (CEP) |
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Term
| Dual color Dual fusion should show what for PML/RARA? |
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Definition
| both Red and Green probes located adjacent to eachother since the translocation occured between the two different chromosomes |
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Term
| What should a Dual color break apart probe show on Inv(16) and what is normal? |
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Definition
| Separation of the colors when normally they should be solid |
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Term
| What does DNA testing for mutations require? |
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Definition
| Some knowledge of the mutations that cause the disease (DNA sequence and location) |
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Term
| Philadelphia Translocation |
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Definition
| t(9;22) CML which moves ABL onto promoter of B-cell Receptor (high activity) |
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Term
| What does the t(9;22) in CML result in in terms of cellular activity |
|
Definition
| Increased Tyrosine Kinase activity of ABL |
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Term
| CML arises in a stem cell that is what precursor? |
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Definition
| Granulocyte precrusor as opposed to lymphoid |
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Term
| What are the three main treatment options for CML? |
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Definition
| Gleevec (imatinib), Hydroxyurea, Alpha interferon |
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Term
|
Definition
| It inhibits the tyrosine kinase activity of the BCR-ABL |
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Term
| If a patient presents with a DIC-like clinical picture think what? |
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Definition
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Term
| What happens to the bone marrow in APL and what is treatment? |
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Definition
| Promyeloblasts proliferate and can't differentiate further, unlessed induced by ATRA |
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Term
| What is the karyotype of APL? |
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Definition
|
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Term
| What will immunophenotypes (through flow cytometry) show on APL? |
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Definition
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Term
| Eosinophil with Basophilic Granules indicates what disorder? |
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Definition
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Term
| What is the clinical picture of Burkitt Lymphoma? |
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Definition
| High fever, night sweats, nausea, anorexia, weakness |
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Term
| What do the B-cells in Burkitt Lymphoma show on smear? |
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Definition
| Deep basophilic cytoplasm, "Stary sky", Lipid vacuoles |
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Term
| Karyotype of Burkitt's Lymphoma |
|
Definition
| t(8;14) resulting in fusion of c-myc |
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Term
| What is the karyotype of ALCL? |
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Definition
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Term
| Typical Immunophenotype results of ALCL? |
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Definition
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Term
| Karyotype of Mantle Cell Lymphoma (MCL) and what protein is involved |
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Definition
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Term
| What will immunophenotype of Mantle Cell Lymphoma invariably show? |
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Definition
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