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Cytochemical Stains
Hematology
10
Biology
Undergraduate 4
11/21/2011

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Term
Myeloperoxidase
Definition
Most sensitive stain for granulocytes
Differentiates AML from ALL
Lymphoblasts do not stain
AML+
ALL-
Term
Sudan Black B
Definition
Differentiates AML from ALL
Lymphoblasts do not stain
Stains primary and secondary granules in granulocytes
Also stains Auer Rods
-Found in myeloblasts and granulocytes
AML+
ALL-
Term
Chloroacetate Esterase Specific Stain
Definition
Differentiates AML from ALL
Negative for Acute Eosinophilic Leukemia (AEL)
AML+
ALL-
Term
Nonspecific Esterase
Definition
Diagnoses Acute Myelomonocytic Leukemia and Acute Monocytic Leukemia
Monoblasts stain intense
Also stains plasma cells, hairy cells, T-lymphocytes
AML+
ALL-
Term
PAS
Definition
Diagnoses ALL, Acute Erythroid Leukemia, and Megakaryoblastic Leukemia
Normal erythroblasts do not stain with this
AML-
ALL is negative or “chunky positive)
Identifies abnormal glycogen metabolism
Term
LAP
Definition
Distinguishes Leukemoid Reactions/Reactive Neutrophilia from CML
CML-
Found in Leukemoid Reactions
Term
TRAP
Definition
Hairy Cell Leukemia is positive
Mast cells are positive
Term
TdT
Definition
Positive in Thymus Lymphocytes and Pre-B Lymphocytes
-Found in ALL
Distinguishes ALL from Malignant Lymphoma
DNA polymerase
Primitive Cell Marker
Term
Toluidine Blue
Definition
Positive for mast cells and basophil cells
Negative for hairy cells
Term
Reticulin Stain and Masson’s Trichrome Stain
Definition
Identifies the presence and extent of fibrosis
-Will see fibrosis in Myelofibrosis
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