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Culture Media
Exam 2 and Final
20
Microbiology
Undergraduate 2
11/07/2010

Additional Microbiology Flashcards

 


 

Cards

Term
EMB
Definition
Eosin Methlylene Blue, selects for G- and lactose fermentation
Term
PEA
Definition
Phenylethyl alcohol selects for G+
Term
MacConkey
Definition
Crystal violet and bile salts select for G-, lactose fermentation is detected by Neutral Red pH indicator
Term
Starch
Definition
Use Iodine as the reagent to detect amalyase activity
Term
Litmus Milk
Definition
Fermentation of lactose, use of casein protein (proteolysis). Litmus is the pH indicator (pink-blue), and redox indicator (white=reduced)
Term
Triple Sugar Iron
Definition
Sugars flucose, sucrose, and lactose, pH is phenol red (Y-R) gases CO2 and H2H may be produced use FES as indicator.
Term
OF medium
Definition
Fermentation of glucose will turn medium yellow (from thymol blue Y-B), indicates fermentation or oxidation
Term
BGA
Definition
Food micro for detection of Gram- lactose fermenters selective agent and indicator is brilliant green dye (Green-Red)
Term
m-Endo
Definition
Selective and differential, basic fushsin selects for G- lactose and sodium silfite and dye differentiate.
Term
Phenol Red
Definition
pH indicator yellow as acid
Term
OF Agar Indicator
Definition
Bromthymol Blue
Term
Brilliant Green Agar Differential Agent
Definition
Lactose
Term
Most Probable Number (MPN)
Definition
Must do conformation and usually for water purity
Term
Gelatin
Definition
shows the presence of gelatinase if there is liquification
Term
IMViC
Definition
indole, methyl red, voges-proskauer, citrate
Term
Indole
Definition
shows pressence of tryptophanase pink ring on top (E. Aerogenes does not have)
Term
Methyl Red
Definition
Yellow Postive (E. Coli) negative (E.aerogenes)
Term
Voges-Proskauer
Definition
E. aerogenes is negative neutral products/ pink is a positive
Term
Citrate
Definition
pH indicator is brom-thymol blue (yellow-blue)- sodium citrate as sole carbon source
Term
Urea
Definition
Urease, positive is red
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