Term
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Definition
| signals the end of transcription |
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Definition
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Definition
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Term
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Definition
| introns and exons both appear in the primary mRNA transcript |
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Term
| nucleic acid hybridization |
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Definition
the method that originally revealed introns exisited.
Two steps:
the target DNA is denatured by heat to break the H-bonds between the base pairs and separate the two strands.
A single stranded nucelic acid from another source is incubated with the denatured DNA. |
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Term
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Definition
| is added to the 5' end of the pre- mRNA as it is transcribed |
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Term
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Definition
| is added to the 3' end of the pre-mRNA at the end of transcription |
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Term
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Definition
| removes the introns and splices the exons together |
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Term
| Small Nuclear ribonucleoprotein particles (snRNPs) |
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Definition
| bind to consensus sequences near the 5' and 3' end |
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Term
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Definition
| a boundary between introns and exons. Short stretches of DNA that appear with little variation |
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Term
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Definition
| using energy from ATP proteins are added to form a large RNA protein complex. Complex cuts the pre-RNA, releases the introns and joins the ends of the exons together ro produce mature mRNA |
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Term
| Prokaryotic Gene expression:Transcription and translation Occurance, gene structure, and modification of mRNA after initial transcription but before translation |
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Definition
Transcription and translation occurrence: at the same time in the cytoplasm
Gene structure: DNA sequence is read in the same order as the amino acid sequence
Modification of mRNA after initial transcription but before translation: None
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Term
| Eukaryotes: transcription and translation occurence, gene structure, modification of mRNA after initial transcription but before translation |
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Definition
transcription and translation occurrence: transcription in the nucleus then translation in the cytoplasm
gene structure: noncoding introns within coding sequence
Modification of mRNA after initial transcription but before translation: introns spliced out 5'cap and 3' A added |
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Term
| True or false: Introns scramble the DNA gene sequence |
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Definition
| False introns only interrupt DNA sequence |
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Term
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Definition
| AAUAAA. Acts as a signal for an enzyme to cut the pre-mRNA |
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