Term
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Definition
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Term
| What is recombinant DNA tecnology? |
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Definition
| lab techniques for combining DNA from different sources into one organism |
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Term
| What is the basic process for genetically modifing organisms? |
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Definition
1) remove gene from cell and put it into DNA in cell of another organism
2)Now you have GM organism that has recombinant DNA
3) as GMO grows/reproduces copies of gene are created through mitosis/meiosis |
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Term
| For 10,000 years humans have done selective breeding on plants and animals and now scientists can create GMOs with very specific traits. What are the differences? |
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Definition
1) time - long v short
2) source of genes - used to be from specific gene pool or close relatives but now they come from diverse sources. |
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Term
| GMO = _______________________ |
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Definition
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Term
| Why did we create Round-up Ready soybeans with herbicide-resistance genes? |
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Definition
| To kill the weeds but not the crop |
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Term
| Why do we have pigs with spinach genes? |
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Definition
| To make them more nutritious when we eat them. |
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Term
| Why did we put human genes into sheep? |
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Definition
| To get treatment for sick humans |
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Term
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Definition
| small circular strands of DNA which separate from the main, larger bacterial chromosome |
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Term
| Plasmids are like pokemon how? |
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Definition
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Term
| How do you know if a bacteria took up your modified plasmid? |
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Definition
| Insert an antibiotic resistance gene with the modified gene and then cover with antibiotics |
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Term
| Where did restriction enzymes come from? |
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Definition
| A bacterial defense against viral DNA |
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Term
| What is a restriction site? |
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Definition
| DNA sequence in target DNA where the enzyme makes stggered cut in the strand |
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Term
| So recap Recominant DNA you do what? |
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Definition
| create rDNA, cut both with the same RE, maix and bond together |
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Term
| What is DNA fingerprinting? |
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Definition
| Comparing the collections of DNA restriction fragments between different samples |
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Term
| Give examples of how DNA fingerprinting would be useful. |
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Definition
| Crime, Unidentified Bodies, Paternity Disputes, Historical Disputes, Research in Ecology and Evolutionary Biology |
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Term
| How is DNA fingerprinting done? |
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Definition
1)Obtain DNA samples
2)RE digest (cut up) and amplify
3)Compare fragments |
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Term
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Definition
| Polymerase Chain Reaction; Amplifies DNA (makes more by copying) |
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Term
| When DNA is heated it ______________ |
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Definition
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Term
| heat = _______________ and more ____________ breaks H bonds between 2 sides |
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Definition
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Term
| To allow DNA to "rezip" you must what? |
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Definition
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Term
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Definition
| Restriction Fragment Length Polymorphism |
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Term
| Polymorhism in genetics is what? |
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Definition
| A sequence of DNA whose ase sequence varies amon people |
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Term
| Why isn't everyones DNA sequence the same? |
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Definition
| Introns and Intergenic Regions have indels that vary from person to person. |
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Term
| What does a gel electrophoresis do? |
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Definition
| Sorts big molecules by size and electrical charge |
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Term
| What are the steps when dealing with gel electrophoresis? |
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Definition
1)a solution with restriction fragments is placed in well in the gel block
2)electrodes at both ends move the negatively charged DNA toward the other end
3)small fragments move fast and large move slow
4) visualize fragments with dye or radiation |
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Term
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Definition
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Term
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Definition
1) Opens the "book" on species histories
2) Helps us understand complex genetics of common diseases |
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Term
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Definition
| Determining the order of bases for a gene or entire genome |
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Term
| How do you do DNA sequencing? |
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Definition
| With modified PCR and gel electrophoresis |
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Term
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Definition
| many copies of 1 fragment are mixed with millions of bases and special versions of bases. |
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Term
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Definition
1) Terminate elongation of fragment
2) They glow with differnt color for each version |
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Term
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Definition
| The use of sequence data, computers and statistical techniques to understand genetics |
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Term
| Bioinformatics ____________ and ______________ huge amounts of data so that the __________________ info is usable for researchers |
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Definition
| manages; organizes; genome |
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Term
| Why would insurance companies want the genome discrimination act to pass? |
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Definition
| They want people to get the test so they can catch disease early and spend less money on treatment |
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Term
BIOTECHNOLOGY
CON or PRO
Technology advances faster than laws |
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Definition
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Term
BIOTECHNOLOGY
CON or PRO
Advances in our understanding of disease |
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Definition
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Term
BIOTECHNOLOGY
CON or PRO
Crime, Exoneration, Paternity Responsibilites |
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Definition
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Term
BIOTECHNOLOGY
CON or PRO
Society must use their resources and those remain ignorant get left behind. |
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Definition
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