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CLS 202 Immunoassays
Imunoassay concepts pertinent to the clinical laboratory.
16
Science
Undergraduate 2
09/21/2012

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Term
what is the functional domain of the antibody
Definition
immunoglobulin
Term
what is antigen also called
Definition
immunogen
Term
what is the epitope or antigenic determinant
Definition
the portion of an antigen involved in the reaction with an antibody
Term
what determines antigenicity
Definition
the tertiary shape of the molecule and the degree of "foreignness" between "self" and the antigen
Term
what is cross-reactive
Definition
when a antigen is structurally similar to the homologous antigen to which it was produced
Term
what kind of reactions are easily visible to the unaided eye
Definition
agglutination reactions
Term
what is the prozone
Definition
when the antibody is in excess
Term
what is the postzone
Definition
when the antigen is in excess
Term
what is the zone of equivalence
Definition
generally when there are 2-3 antibody molecules per each antigen
Term
what assay is charachterized by measurable rings of precipitaiton
Definition
Radial immunodiffusion
Term
what is a drawback of radial immunodiffusion
Definition
the long diffusion period
Term
what is the assay where two wells are cut into a plain gel and the antibody and antigen diffuse toward each other
Definition
Double diffusion technique
ouchterlony technique
Term
what is used to identify monoclonal proteins in serum and urine
Definition
Immunoelectrophoresis
Term
when do a fixed amount of labeled antigen competes with unlabeled sample antigen for a limited number of antibody sites
Definition
competitive immunoassays
Term
why does charcoal work as a separating agent
Definition
because it can suck up the small particles that are unbound
Term
how do protien precipitants work as a separating agent
Definition
reacts with antibody in the system causing the free ones to precipitate and free antigens remain in suspension
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