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Clinical Instrumentation - Fluorometry
Clinical Instrumentation - Fluorometry
9
Medical
Undergraduate 2
03/24/2012

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Cards

Term
Fluorometry - Use
Definition

using UV radiation, some substances absorb the radiation and get excited

 

after excitation they return to ground state and emit a photon of light

 

fluorescent light results from absorbace of the photon of radiant energy

Term

Fluorometry - Set Up

Definition
lamp --> entrance slit --> primary monochromater --> exit slit --> sample --> entrance slit --> secondary monochromater --> exit slit --> photo detector --> read out
Term

Fluorometry - Lamp

Definition

gas discharge (mercury or xenon)

 

incandescent lamps can't be used as they release little UV light

Term

Fluorometry - Cuvette

Definition

made of quartz as it is transparent to UV wavelengths

 

fluorescing sample emits light in all directions

Term

Fluorometry - Primary Filter

Definition
selects the wavelength that is best absorbed by the solution to be measured
Term

Fluorometry - Secondary Filter

Definition

placed at right angle to sample cell so it can pass longer wavelengths of fluorescent light and prevent incident light from hitting photodetector

 

 

Term

Fluorometry - Monochromators

Definition

gratings

 

prisms

 

filters for isolation of radiation

Term

Fluorometry - Photodetector

Definition
photomultiplier tubes because of their higher sensitivity to low light intensities
Term
Stokes Shift
Definition
the difference in wavelengths between the band maximas of the absorption spectra and the emission spectra
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