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Clin Lab 5
From Quiz 4 to the final
130
Health Care
Professional
03/23/2012

Additional Health Care Flashcards

 


 

Cards

Term
How is acute laryngitis diagnosed?
Definition
based on clinical features
-many viral causative agents which can also be tested by a fast strep test
Term
When laryngitis is caused by TB, how can it be tested?
Definition
#1: laryngeal bx
-also culture, blood culture, CXR
Term
What is the problem with mycobacterium tuberculosis?
Definition
-doesn't stain easily
-very very slow growing
-histo likes to hang out in old TB lesions.
Term
When would you want to put somebody with laryngitis on abx?
Definition
when they have a comorbidity.
Term
What diseases can present in the pharynx/ mouth?
Definition
Herpes gingivostomatitis
-Rapid antigen detection of moist lesion
-Lesions may be cultured
-HSV is primary

Herpes Labialis
-Rapid antigen detection of moist lesion
-Lesions may be cultured
-HSV is primary

Oral thrush
-KOH or gram stain of oral lesion
-Candida albicans

Strep Pharyngitis
-Rapid antigen test for S. pyogenes specific but varies in -sensitivity; culture if negative
-Viruses most common cause
-Rx: abx: penicillin

Corynebacterium diphtheria
-Culture
-Antitoxin + erythromycin
-We get the vaccine for this when we ae younger… it is the toxoid to what we make the immune rxn to this.

Epiglottitis
-Usually seen in children
-Sore throat drooling
Term
What microbe likes IV lines?
Definition
pseudomonas
Term
What is the causative agent in otitis externa? How would you test for it and how would you tx it?
Definition
Pseudomonas, Staph aureus, strep pyogenes
Gram stain; C&S
1st Cortisporin; 2nd Ciprofloxacin otic
Term
What is the causative agent in otitis media? How would you tx it?
Definition
-Strep pneumoniae, H. influenzae, M. Catarrhalis, are the most common causes; others include Strep pyogenes, Staph aureus
-Amoxil 80-90 mg/kg/day
Term
What is the causative agent in orbital cellulitis? How would you treat it?
Definition
-Staph aureus, Strep pyogenes, H. influenzae
Strep pneumoniae
-Nafcillin + Rocephin + Flagyl
Term
How would you treat acute sinusitis?
Definition
Try decongestants x 10 day unless otherwise indicated
Augmentin
3rd generation cephalosporin
Term
What are the causative agents in the common cold? What is the tx?
Definition
Rhinovirus, coronavirus and adenovirus + others

Tx the symptoms not the infection
Term
What are the risk factors for nosocomial pneumonia?
Definition
Hospital Acquired:
Endotracheal intubation
>70 yrs
Depressed mental status
Primary cause is klebsiella and others.
Term
What are tests to do to find the primary cause of nosocomial pneumonia?
Definition
Leukocytosis w/left shift
Sputum gram stain: predominance one organism;
-increased PMN’s
CXR: expanding infiltrate
Decreasing PaO2
Term
What are the causative agents of pneumonia in the following people: adults?
young child?
young adult?
hospital?
Definition
-staph and strep
-viral: influenza A&B
-mycoplasma
-klebsiella
Term
What does bordatella pertussis look like? How do you test for it and how do you treat it?
Definition
Gram negative rod or coccobacillus

Culture or PCR from nasopharyngeal specimen
CXR may show pneumonia

Azithromycin; clarithromycin; septra
Term
How do you test for and tx klebsiella?
Definition
Sputum C&S; gram stain
Doripenem; Imipenem
Term
How do you test for and trest H. flu? What is it the most common cause of in infants?
Definition
Sputum C&S; gram stain
Ampicillin IV; Amoxil p.o.
In infants is the most common cause of meningitis
Term
How many serotypes are there for influenza? Which is the nastiest?
Definition
6: A,B,C,D,E,F
-B is the nastiest and there is a vaccination for this.
Term
Where is Klebsiella pneumoniae present? What can it cause? How is it treated?
Definition
K. pneumoniae is present in the respiratory tract and feces of about 5% of normal individuals.

-Can produce extensive hemorrhagic necrotizing consolidation of lung
-Occasionally causes UTI and bacteremia
-Involved in hospital acquired infections
-Urine catherters

Rx
-Ampicillin + gentamycin
Term
What are the tests for legionella pneumophilia and how is it treated?
Definition
C&S Sputum; Urinary antigen test is rapid and sensitive
Azithromycin IV
Term
What are all mycobacteria?
Definition
acid-fast stain or cultures
Term
What is mycobacterium avium-intracellulare present in and how is it treated?
Definition
HIV
clarithromycin
Term
How does atypical pneumonia present and what are the causes?
Definition
Subacute in onset
Cough is nonproductive
“Walking pneumonia”
Coud be walking around with this for several weeks.

Primary causes:
Mycoplasma pneumoniae (Young adults)
Chlamydophila pneumoniae
Respiratory viruses: Influeza, adenovirus, parinfluenza, RSV
Term
What is kingella kingae and what does it cause?
Definition
Fastidious GN coccobacillus
Colonizes Respiratory – Oropharynx in children

Osteomyelitis (mobile baby child)
Septic arthritis (mobile baby child)
Bacteremia (infants)
Endocarditis (Older child)
Term
When a baby who is walking is infected by Kingella what are they going to do?
Definition
revert back to crawling.
Term
Among who does adenovirus serotypes 3,4,7 acutely cause respiratory disease? What tests would you want to do if suspecting it?
Definition
military recruits
-Adenoviral culture from respiratory specimen
-Rapid viral antigen detection
Term
What are adenovirus serotypes 40 & 41 associated with?
Definition
infantile gastroenteritis.
Term
What does the CXR of RSV look like? What tests would you do? What is the TX?
Definition
May show interstitial infiltrates
Viral culture or rapid viral detection
Treatment supportive
Term
What is the difference bw the branching of the microorganism in adenovirus and aspergillis?
Definition
-adenovirus: 90 degrees
-aspergillus: 45 degrees
Term
Where is histoplasmosis picked up?
Definition
Midwest and SE U.S
Moist soil; OH and Mississippi river valley
Caves and old buildings
Term
What does histoplasmosis cause? What is positive for it half the time? What is 2 tests are most sensitive? How do you treat it?
Definition
90% brief self - limiting flu like illness

Bone marrow positive in 50% of cases

Polysaccharide urine and serum antigen test is most sensitive
Urine antigen test positive in 90% of disseminated histoplasmosis

TX:usually self-limiting, but can use Iatroconazole
Term
Where/how can a person pick up coccidiomycosis?
Definition
-Dry alkaline soil, hot summers w/few winter freezes
-Central CA, S. Arizona, NM and TX, Mexico, Central and S.A.
-Dust storms, evacuation, earthquakes
-Inhaled releasing endospores transported to hilar lymphnodes and blood
Term
How does coccidiomycosis manifest on the skin and in the lungs?
Definition
-Skin manifestations: erythema nodosum, erythema multiforme, nonpruritic papular rash
-Chronic lung disease can lead to fibrosis, nodules, cavities
Term
How can you test for coccidiomycosis and how is it treated?
Definition
Serology tests measure IgG and IgM
Rx: Fluconazole; in severe disease Amphotericin B
Term
What is serratia (S. marcescens)? Who is it especially in? What is the tx?
Definition
-opportunistic pathogen
-Pneumonia, bacteremia, endocarditis especially in narcotic addicts and hospitalized patients

-TX: 3rd generation cephalosporin (often resistant to aminoglycosides and penicillin
Term
What are the six ways in which you can test for TB? Note the gold standard.
Definition
PPD
-In the presence of compatible CXR and clinical findings, a positive PPD in an untested patient suggests TB

Microscopy
-Acid fast bacilli

Mycobacterial culture
***Gold standard--- but will be waiting and waiting.
-Sputum – solid and liquid media most sensitive

Nucleic acid amplification
-Rapid detection of TB but does not replace culture

CXR
-May detect adenopathy, effusion, cavitation, nodules

Anatomic pathology
-Caseating granulomas may be observed in biopsies of enlarged lymph nodes.
-Caseating (necrosis) granuloma (an inflamm response)
Term
What is the difference bw TB and miliary TB on a CXR?
Definition
-TB has coin lesions
-miliary TB will look like pneumonia
Term
In what 5 ways can you test for Legionnaire Dz?
Definition
Bacteriologic culture
***Gold standard

Urinary antigen
--Detects only serogroup 1

Direct fluorescent antibody test
--Performed on colonies isolated from bacteriologic plates

Serologic
--Rising titers of antibody to legionella may be useful in documentation of disease in culture negative cases

PCR analysis
Term
How would you test for nocardiosis?
Definition
Microbiology
-Gram stain, modified acid fast stain or aerobic culture
Sputum or bronchial specimen

Anatomic pathology
-Biopsy
Term
What are 2 systemic mycotic infections?
Definition
blastomyces
coccidioides
histoplasma
candida
cryptococcus neoformans
Term
How do you test for blastomyces?
Definition
Lab: KOH, Culture, DNA hybridization
Histology: Broad based budding yeast in tissues
Term
How do you test for coccidioides?
Definition
Lab: Endospores or spherules may be visible in wet mounts
Culture; DNA hybridization; Serological testing
Histology: Spherules with endospores may be seen in tissue
Term
How do you test for histoplasma capsulatum?
Definition
Lab: Budding yeast or intracellular forms within macrophages
Culture; DNA hybridization; serologic testing
Term
How do you test for candida?
Definition
Lab: KOH; Cultures
Histology: pseudohyphae or hyphae in infected tissue
Term
How do you test for cryptococcus neoformans?
Definition
Antigen detection by latex agglutiniation of CSF
India Ink smear may detect yeast
Confirmatory culture using CSF
Term
When testing CSF what is in Tubes 1,2,3,4?
Definition
Tube 1: Protein, Glucose
Tube 2: Gram Stain & Culture
Tube 3: Save for further testing
Tube 4: Cell count, Color, Clarity and opening pressure (100-200 mm Hg)
Term
What are some viral infections of the GI tract? bacterial?
Definition
viral: rotovirus, adenovirus, cytalomegavirus, norwalk virus

bacterial: campylobacter jejuni, e. coli, myobacterium avium-intracellular, h. pylori, salmonella enteritidis, shigella, vibrio cholerae, yersinia enterocolitica
Term
How do you test for rotavirus?
Definition
Lab: Direct antigen detection in stool specimen by EIA
Term
How do you test for adenovirus?
Definition
Not culturable
EIA must be used
Term
How do you test for CMV?
Definition
May be cultured from colonic biopsies
Term
How do you test for norwalk virus?
Definition
RT-PCR is method of choice
Term
How do you test for Campylobacter jejuni?
Definition
Fecal WBC’s, Gram Stain, Culture
Term
How do you test for e.coli?
Definition
Culture stool; O & H serotyping
Term
How do you test for myobacterium avium-intracellular?
Definition
-blood cultures
-Lymph node, bone marrow, liver biopsies may reveal AFB
Term
What is the gold standard when testing for H. Pylori?
Definition
biopsy
Term
How do you test for salmonella enteritidis? What does this bacteria cause?
Definition
Stool culture; Fecal WBC

Can cause bloody or non-bloody diarrhea
There is a carrier state
Does not ferment lactose
Term
How do you test for Shigella? What does this bacteria cause?
Definition
Stool Culture; fecal WBC

Always produces bloody diarrhea
2-5 days, self limiting
Not a large carrier pop
Very toxic, inoculum does not have to be very great
Term
How do you test for vibrio cholerae? What does it cause?
Definition
Stool Culture; serotype

Profuse watery diarrhea
Has a relative vibrio parahemolyticus (contaminated aquatic food items)
Term
How do you test for yerisinia enterolitica?
Definition
stool culture.
Term
What is the most common manifestation of salmonella infection? What else is prominent causes of this?
Definition
-enterocolitis
-In the USA, S. Typhimurium and S. Enteritidis are prominent.
and canbe caused by any of the 1400+ group I serotypes of salmonellae
Term
What is the tx for enterocolitis?
Definition
Ampicillin
TMX-SMZ
3rd generation celphalosporin
Term
When is salmonella pathogenic?
Definition
when acquired from the oral route
-transmitted from animal products to humans
-produce hydrogen sulfide
-powerful: only need a small inoculum to be pathogenic
Term
What are the 4 salmonella strains? Which one is nasty among sickle cell patients? Which one produces hydrogen sulfide?
Definition
Salmonella Paratyphi A
Salmonella Paratyphi B
Salmonella Choleraesuis C1
-Particularly nasty among sickle cell patients
Salmonella Typhi D
-Produce hydrogen sulfide.
--In a test tube, get a dark line in your media, this is your hydrogen sulfide.
Term
Where is the natural habitat of the shigellae? What do they produce here?
Definition
limited to the intestine of humans and other primates where they procude bacillary dysentary
-Infections are almost always limited to the GI tract
Term
What do shigellae look like? What kind of anaerobes are they? What do they ferment?
Definition
Slender gram negative rods
Facultative anaerobes
Ferment glucose
Term
What is the incubation stage of shigella? What are the S/S?
Definition
-Sudden abdominal pain, fever, watery diarrhea due to exotoxin
-Each BM is accompanied by straining and rectal spasms
Term
When do more than half resolve? While few remain what? If does not resolve, which treatments can you use?
Definition
-More than half resolve in 2-5 days but most shed dysentery bacilli for a short period
-Few remain chronic carriers

Rx:
Cipro
Ampicillin, TMX-SMZ
Avoid opiods
Supportive
Term
When someone has food poisoning, what is their vomit like?
Definition
big smelly chunks
Term
What are some causes of food poisoning?
Definition
Vibrio

Bacillus cerus
-Chinese buffet.. Fried rice.. 24 hours later N/V/D
-Self-limiting

Staphylococcus aureus
-Potato salad… anything with mayonaise

Clostridium Botulinum
-Infant: honey with botulinum spores
-Adult: dented can, or can your own food.
Term
What does B. cereus cause? how long does it last?
Definition
emetic (so vomiting)
diarrheal

-self limiting 24 hours
Term
What are the treatments for B. cereus?
Definition
Penicillin resistant
Vancomycin, Clinda, FQ
Supportive
Term
What do staphylococci look like? Some members are part of the normal flora of what 2 things?
Definition
-Gram positive cocci; grape like clusters
-skin and mucus membranes
Term
*What do pathogenic species of staphylococci do?
Definition
hemolyzes blood and coagulates plasma
Term
What is the most common type of food poisoning caused by?
Definition
heat stable staphylococcal enterotoxin
Term
How many species of staphylococci are there and what can they develop rapidly?
Definition
30
-resistance developed rapidly
Term
What do staph and strep have in common? different?
Definition
-both can hemolyze blood
-only staph is catalase positive
Term
When treating staph, why do you have to pick a penicillin it is not resistant to?
Definition
because it produces beta lactamase (90% of them)
Term
What is staph aureus (20%) and s. epidermis (75%) resistant to?
Definition
nafcillin
methicillin
oxacillin
Term
What 4 other things can staph aureus produce?
Definition
Alpha toxin is a potent hemolysin

E leukocidin
-Kills WBCs, so can evade immune systems.

Produces TSST-1

Produces enterotoxins (A-E, G-I, K-M)
Term
Where does staph present in 20-50% of cases?
Definition
nares
Term
Where is staph regularly found? and when?
Definition
-clothing and bed linens
-when person is susceptible
Term
What is the tx for s. aureus infection? What are only a small % susceptible to?
Definition
Acne – Tetracycline
MRSA – Vancomycin, gentamycin, rifampin

Small % susceptible to penicillin G
Term
What does listeria monocytogenes look like?
Definition
short gram positive non-spore forming rod
Term
How does listeria monocytogenes affect the body?
Definition
Important cause of a wide spectrum of disease in animals and humans

Enters GI tract through contaminated food such as cheese or vegetables

Bacteria is phagocytized, releases listeriolysin O which destroys WBC releasing listeriae to attack other cells

-associated with packed meats
Term
What are some pathologies caused by listeria monocytogenes and how do you treat them?
Definition
Granulomatosis infantispectica
Listeria meningocephalitis
--Among our young.
Bacteremia

Rx:
Ampicillin + EES
Ampicillin + gentamycin
Term
What are the 4 types of e. coli?
Definition
enteropathogenic
enterohemorrhagic (enterotoxic)
enteroinvasive
enteroaggregative
Term
What does enteropathogenic E. coli (EPEC) cause?
Definition
Important cause of diarrhea in infants
Identified by O and H antigen
Term
What does enterohemorrhagic e. coli cause (EHEC)?
Definition
Travelers diarrhea (watery)
Traveler’s diarrhea is not bloody.
Term
What does enteroinvasive E. coli (EIEC) cause? (3)
Definition
Produces verotoxin/associated w/hemorrhagic colitis, acute renal failure

0157.H7- particular strain that is nasty and invasive… ---Jack in the Box (numbers refer to ags on the organism)
-No WBCs in feces.

HUS- hemolytic uremic syndrome… particularly nasty sequale
Term
What does enteroaggregative E. coli (EAEC) cause?
Definition
acute and chronic diarrhea> 14 days.
Term
What are enteric gram negative rods like (enterobacteriaceae)?
Definition
Short gram negative rods
Reside – intestinal tract
Includes: escherichia, shigella, solmonella, enterobacter, klebsiella, serratia, proteus
Facultative anaerobes or aerobes
Some are lactose fermenting others are not
Term
What are the antigenic structures of enteric gram negative rods?
Definition
O : most external part of cell wall LPS

K : external to O antigens on some not all
Associated with our cell wall just outside of our O

H: located on flagella
Term
What are the 5 protozoal infections?
Definition
Flagellates (giardia lambia)
Ameba (entamoeba histolytica)
Coccidia
Ciliates
Term
How is giardia transmitted? What S/S does it cause? What labs should be ordered and what is the tx?
Definition
-water and person to person transmission

-diarrhea, abd. cramps, bloating, flatuence

Labs: ova and parasites

Tx: Flagyl
Term
In what 3 ways does entamoeba histolytica manifest?
Definition
Asymptomatic colonization – diarrhea

Amebic colitis/dysentery
-#1 cause of dysentery

Extraintestinal abscess usually in the liver
-Once drained is anchove paste like.
Term
What labs would you order for entamoeba histolytica and what is the tx?
Definition
-ova and parasites; serological tests if available
-not self limiting, can cause death!
--Flagyl
---Iodoquinol (Yodoxin) if Flagyl fails
Term
Microscopically the ameba has 2 dots, one is the nucleus. What is the other?
Definition
RBCs (ingests RBCs and causes bloody diarrhea)
Term
What is Crowder's story for entamoeba histolytica?
Definition
refugee camp due to poor sanitation…. Have accidentally consumed contaminated water.
Term
Ameba's relative is Neigleria floweri.. what's Crowder's story on this?
Definition
FL, AL, GA: summer, kids have been down to the local swimming hole.. Child in the evening will have the worst HA ever… 24 hrs later the child is dead
-this is meningoencephalitis
Term
In what 3 ways can coccidia present?
Definition
Cryptosporidium spp.: drinking water; recreation pool
-Relatively common cause of infection in US
-Mid-80s in Milwaukee… waste was spilling into drinking water

Cyclospora spp.: imported foods - raspberries

Isospora belli – diagnosised in immunosuppressed patients
-Self-limiting diarrhea
Term
What labs would you order for coccidia? Treatments?
Definition
Acid fast stain or direct fecal antigen (DFA)

Rx:
Nitazoxanide has been FDA-approved for treatment of diarrhea caused by Cryptosporidium in people with healthy immune systems
Term
How do ciliates present?
Definition
Balantidium coli
People who have traveled to tropical places that have poor sanitary conditions
Immigrants from tropical countries that have poor sanitary conditions
People who live in institutions that have poor sanitary conditions
Term
What is the labs and tx for ciliates (balantidium coli)?
Definition
Labs:
Request Ova and Parasites
Look for cilia.
Wet mount

Rx: Tetracycline
Term
How does microsporidia present?
Definition
More closely related to fungi
Enterocytozoon bienusi
-Self limited diarrhea; chronic diarrhea in AIDS patients
Term
What are the labs and tx of microsporidia?
Definition
Chromotrope-based staining of stool

Rx: Albendazole
Term
what are 6 helminth infections of the GI that are treated by Prazinquantel or niclosamide?
Definition
Diphyllobothrium latum
-Fish tapeworm
--sushi

Taenia saginata
-Beef tapeworm
--No hooks
--Beef tapeworm proglottid is bigger

Taenia solium
-Pork tapeworm
-Has hooks and a smaller proglottid
--Has a nasty larval form-->can cause cysts in the brain… shouldn’t be there bc are used to being in a pig. They die in the brain and release toxins there.
---these cysts can calcify
Term
What is the enterobius vermicularis hemlminth infection of the GI? how is it treated?
Definition
Pinworm
Cellulose tape test
Spread by fecal-oral route.

Rx
Mebendazole; albendazole
Term
What is the Ascaris lumbicoides helminth infection of the GI?
Definition
Uncommon in US
-Other countries use human feces as soil to grow veggies.

Heavy infections can cause intestinal blockage and impair growth in children
Term
What is the strongyloides stercoralis helminth infectoin of the GI?
Definition
Tropical and subtropical areas, but cases also occur in temperate areas (including the South of the United States). 

More frequently found in rural areas, institutional settings, and lower socioeconomic groups.

Necater-->hookworm
Term
What is Crowder's story with the hookworm (strongyloides stercoralis)?
Definition
Kids are outside in the summer not wearing their flip-flops or tennis shoes and makes its way up to lungs.
Term
How can both ascaris lumbicoides and strongyloides sterocoralis be treated?
Definition
benzadole (treatment for most helminths actually)
Term
What is the "Trichuris trichiura" helminth GI infection?
Definition
"whip worm"
-heavy symptoms can experience frequent, painful passage of stool that contains a mixture of mucus, water, and blood.
-Rectal prolapse can also occur.
-Children with heavy infections can become severely anemic and growth-retarded
Term
Which GI helminth infection causes disease of the bladder?
Definition
Schistosoma haematobium
Term
What does shistosoma japonicum and shistosoma mansoni cause? What is the difference?
Definition
N/V, hemoptysis, melena, hepatosplenomegaly, portal hypertension

-mansoni is found in the Caribbean, S. America, Africa, Middle East
Term
What is Crowder's story on S. mansoni?
Definition
Going barefoot through an Asian rice farm
Potential sequelae is bladder CA
Like to hang around in veins around GI tract
Term
What is the difference in how the 3 shistosomas look?
Definition
haematobium- lateral spike (spike is really little

Japonicum- has a little spike at the end

Mansoni- lateral spike on an egg (spike is big and nasty)
Term
What are the 3 ways in which you can evaluate a UTI?
Definition
UA
-Positive leukoesterase
-+/- nitrite

C&S
-80% E.coli

Ureteroscopy
-Children
-Recurrent UTI
Term
Where are three common sites of infection in the male genital tract?
Definition
seminal vesicles
epididymo-orchitis
prostate
Term
What is usually the causative agent in the seminal vesicles?
Definition
GNR and N. gonorrhoeae
Term
What are epididymo-orchitis infections most often caused by? What causative agents are involved that can possibly cause sterility and loss of testes?
Definition
-most often caused by STI
-Chlamydia, GNR, N. gonorrhoeae, M. tuberuculosis
Term
What are the tx for infections in the epididymo-orchitis?
Definition
rocephin + doxycycline
Term
What are the typical causative agents of the prostate?
Definition
GNR, Enterococci, Staph, and N. gonorrhoaea
Term
How do you tx a prostate infection if the person is <35 yoa? >35yoa?
Definition
-rocephin + doxycycline
-cirpofloxacin or Septra
Term
What are 3 infections that can occur in the female genital tract?
Definition
vulvovaginitis
vaginosis
cervicitis, PID
Term
What are the causative agents in vulvovaginitis?
Definition
Candida albicans, Trichomonas vaginalis (looks like giardia but with one nucleus)
Term
What labs would you do for vulvovaginitis? what treatments?
Definition
-wet prep and KOH
-fluconazole then flagyl
Term
What are the causative agents in vaginosis?
Definition
Polymicrobial (multiple anaerobes and Garderella vaginalis)
Term
What are the diagnostic agents in vaginosis?(3) How would you treat it?
Definition
-Clue cells; odor; pH >4.5
-Metronidazole
Term
What are the causative agents in cervicitis, PID?
Definition
Chlamydia trachomatis, N. gonorrheae
Term
what tests do you run when you suspect cervicitis? What are the treatments?
Definition
culture and a DNA probe

tx:Metronidazole + Doxycycline + Rocephin
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