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| Explain column chromatography |
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| Products are seperated by charge (if the resin is negative the less positive charged will elute) |
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| What is Sanger's reagent? |
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- Came up with how to tell the amino acid sequence
- He added his reagant with HCl which would cut the protein and attach the amino acyl
- Discovered the sequence for insulin
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- His reagant did not require HCL to cut the protein, it was done automatically
- chopped the amino acyl at the n-terminus
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| What is another method used to figure out an amino acid sequence besides Sanger and Edman's methods? |
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Definition
- Break the amino acid up into segments using chymotrypsin
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| The subrate concentration at 1/2 Vmax |
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| What is the shape of the graph for an allosteric inhibitor? |
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| In terms of enzymes what do modifiers do? |
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- bind in a place other than the active site and change the shape
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| Which inhibitors can be removed and which cant? |
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- Noncompetitive inhibitors can be removed via chemical reactions
- Suicide inhibitors cant be removed, they destroy the whole enzyme
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| N-acetylglucosamine (B1--4) N-acetylglucosamine |
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| B-Glucuronic acid (B1--3) N-Acetylglucosamine (B1--4) |
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| B-Glucuronic acid (B1--3) N-acetylgalactosamine sulfate (B1--4) |
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| GAG's are composed of...? |
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| amino sugars and uronic acids |
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| How long after a heart attack is it appropriate to measure the CPK? |
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| How long after a heart attack is it appropriate to measure the LDH? |
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| 1-F.A. + 1-PO4 + Choline= |
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