Term
| glucose oxidase procedure |
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Definition
| you have to have at least 5 test tubes (blk, L1, L2, Std, and Patient) filled with glucose liquicolor reagent. Then add correct ingredients to the labeled test tube and mix and incubate. Record the absorbances at 500nm then calculate the concentrations of the controls and the patients using beers law. |
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Term
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Definition
| interfering substances like hemoglobin, uric acid, ascorbic acid, and bilirubin |
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Term
| The reference range of concentration of glucose oxidase |
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Definition
|
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Term
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Definition
| have predetermined values with reference ranges. They are used to verify accurate test performance. |
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Term
| The principle of the glucose oxidase procedure |
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Definition
| glucose oxidase enzyme will convert glucose in to a product, which can be used in another reaction to produce a colored complex. The colored complex can be measure spectrophotometrically and the absorbance is proportional to the amount of glucose in the sample. |
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Term
| The concentration of glucose is calculated by using beer’s law |
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Definition
| Cu = Au/As x Cs, Cs is given, answer is in mg/dL |
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Term
| Are the results accurate? |
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Definition
| if controls are within range, then yes |
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Term
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Definition
| need to compare run 1 to run 2, are the results similiar |
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Term
| What do you do if the controls are out of range? |
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Definition
| trouleshoot the procedure, figure out what went wrong. |
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Term
| What do you do if one of your unknowns is greater than 400 mg/dL |
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Definition
| dilute to 1:2 and re-run, remember to multiply results by 2 |
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Term
| Glucose levels in spinal fluid are used to diagnose |
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Definition
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Term
| Example of a monosaccharide |
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Definition
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Term
| Example of a disaccharide |
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Definition
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Term
| Example of a polysaccharide |
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Definition
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Term
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Definition
| beta cells of the pancreas |
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Term
| During a brief fast, the glycogen stores of the _____ are drawn upon to release glucose |
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Definition
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Term
| Other than glucose oxidase, name 2 other methods for glucose determination |
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Definition
| glucose-1-dehydrogenase and hexokinase |
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Term
| Three pathogenic conditions causing a rise in glucose concentration in the serum |
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Definition
| diabetes, hyperthyroidism and cushing's disease |
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Term
| Three pathogenic conditions causing a decrease in glucose concentration in the serum |
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Definition
| tumors, addison's and hypothyroidism |
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Term
| Adult reference range for plasma or serum |
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Definition
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Term
| Adult reference range for whole blood |
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Definition
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Term
| Adult reference range for CSF |
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Definition
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Term
| Adult reference range for urine |
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Definition
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Term
| Blood drawn for glucose determination should be centrifuged within 1 hour due to__________, which ______the glucose level |
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Definition
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Term
| CSF should be analyzed for glucose without delay due to _______, which _________ the glucose level |
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Definition
| bacterial contamination, glycolysis and decrease |
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Term
| Why does glycosolated hemoglobin reflect long term control of the diabetic |
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Definition
| measurement of the percentage of hemoglobin bound to glucose which is indicative of the amount of glucose over the lifespan of the RBC |
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Term
| A urine specimen for glucose determination should be collected in a ________ medium in order to suppress ____________ |
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Definition
| acidic, bacterial activity (glycolysis) |
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