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Chemistry Internship Study Questions Section 1
Glucose
30
Chemistry
Undergraduate 4
10/23/2015

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Term
List the reference ranges for glucose in the following:

Plasma, Adults
Definition
70-110 mg/dL
Term
List the reference ranges for glucose in the following:

Plasma, Newborns
Definition
50-100 mg/dL
Term
List the reference ranges for glucose in the following:

CSF
Definition
40-70 mg/dL
Term
List the reference ranges for glucose in the following:

Whole Blood
Definition
65-95 mg/dL
Term
What are the Glucose Critical Values for an adult?
Definition
<50 mg/dL
>450 mg/dL
Term
What are the Glucose Critical Values for a Newborn?
Definition
<45 mg/dL
>180 mg/dL
Term
Why do plasma and whole blood glucose levels differ slightly?
Definition
Whole blood is ~10% less DUE to the Dilutional Effect of RBC
Term
If given a plasma glucose level, how can you estimate the CSF level?
Definition
CSF Level ~ 60-70% Plasma Level
Term
If a sample cannot be tested right away, what is the preferred collection tube/preservative?
Definition
Gray Top
Term
If a sample cannot be tested right away, why would a Gray Top be used?
Definition
NaFl slows Glycolysis
Term
How will a glucose level be affected if an unpreserved whole blood sample is allowed to sit at room temperature for 1 hour?
Definition
Decreases by 10% or more per hour
Term
Name the ONE HORMONE that will DECREASE a Plasma Glucose Level
Definition
Insulin
Term
Name 3 Primary Hormones that can INCREASE a Plasma Glucose Level
Definition
Glucagon, Epinephrine, Cortisol
Term
According to the American Diabetes Association Criteria, what FASTING GLUCOSE level is DIAGNOSTIC for DIABETES?
Definition
>126 mg/dL
Term
What 2 Hour Post-Pradial Glucose Level is Consistent with Diabetes?
Definition
>200 mg/dL
Term
Following the start of an oral glucose tolerance test, at what time should values return to normal levels in NON-DIABETIC?
Definition
2 Hours
Term
What are the MOST common causes of Hypoglycemia?
Definition
Insulin Overdose
Insulin Shock
Term
What are ketones?
Definition
Products of faulty or excessive fat metabolism
Term
In what conditions can you detect ketones in serum or urine?
Definition
Diabetes Mellitus Type I, Vomiting, Starvation, Carb-Free Diets
Term
What is the purpose of performing a glycosylated hemoglobin (HBA1C) assay?
Definition
Estimate Average concentration blood glucose for the preceding 2-3 months; can help determine patient compliance with treatment.
Term
What is the specimen requirement for a HBA1C assay?
Definition
EDTA Whole Blood;
Prepare a hemolysate
Term
List diseases or conditions that can cause a Falsely decreased HBA1C assay
Definition
Hemoglobinopathies, Diseases with INCREASED RBC Turnover, Acute or Chronic Blood Loss, Alcohol, Lead (Pb), Drugs
Term
State the 2 MOST frequently used methodologies for Glucose determination
Definition
Hexokinase & Glucose Oxidase
Term
What is/are the MOST common interfering factors in the following for glucose determination:

Hexokinase
Definition
Bilirubin
Hemolysis
Term
What is/are the MOST common interfering factors in the following for glucose determination:

Glucose Oxidase
Definition
Abscorbic acid
Uric acid
Bilirubin
Term
What may the following common interfering factors of Glucose Oxidase for glucose determination cause:
Ascorbic acid
Uric acid
Bilirubin
Definition
False Negative
Term
Of what significance is a positive urine microalbumin in a diabetic?
Definition
Indicator of early renal disease in the absence of a Positive urine protein dipstick
Term
Describe Type I Diabetes in regards to typical age, major symptoms, and insulin dependence.
Definition
Juvenile on set
Absolute Insulin dependent
Sudden
Weight loss
Ketosis common
Autoimmune
Etiology may be viral
Term
Describe Type II Diabetes in regards to typical age, major symptoms, and insulin dependence.
Definition
Adult on set
Obesity
Sedentary lifestyle
Strong genetic link
Term
What condition is a MAJOR Immediate Risk to new babies born to Diabetic Mothers?
Definition
Insulin shock causing hypoglycemia/seizures
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