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Chapter 8 - Bio 111
Test 2
79
Biology
Undergraduate 3
10/21/2013

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Term
What is the chemical equation of photosynthesis?
Definition
6CO2 + 12H2O -> C6H12O6 + 6H2O + 6O2
REVERSE OF RESPIRATION
Term
Who carries out oxygenic photosynthesis?
Definition
Cyanobacteria
Algae
Terrestrial Plants (chloroplasts)
Term
What is the site of photosynthesis?
Definition
Chloroplasts
Term
____________ membranes contain chlorophyll and other pigments clustered into photosynthesis.
Definition
Thylakoid membranes
Term
What is a grana?
Definition
the stacks of thylakoids embedded in the stroma of a chloroplast.
Term
What is a stroma?
Definition
semiliquid substance surrounding the thykaloid membrane that houses enzymes needed to assemble organic molecules from CO2 using energy from ATP coupled with reduction of NADPH.
Term
What are light dependent reactions?
Definition
Require light captured from sunlight and make ATP and reduce NADP+ to NADPH
Term
What are light INdependent reactions? (Carbon fixators)
Definition
Does not require light DIRECTLY. Uses ATP and NADPH to synthesize organic molecules from CO2
Term
What happens in the light dependent reactions?
Definition
Photosystems in the thykaloid absorb photons of light and use this energy to create ATP and NADPH. Electrons lost from the photosystems are replaced by the oxidation of water, producing O2 as a by-product. The ATP and NADPH produced by the light reactions is used during carbon fixation in the Calvin cycle in the stroma.
Term
What did Helmont discover?
Definition
Plants gain weight, more than just root uptake.
Term
What did Priestly discover?
Definition
PLants release something to restore air.
Term
What did Housz discover?
Definition
Photosynthesis splits co2 and releases Oxygen
Term
What did Blackman discover?
Definition
Photosynthesis occurs in 2 stages: light dependent vs. independent using enzymes
Term
What did van niel propose?
Definition
CO2 + H2A + light energy -> CH2O + H2O + 2A

Later, water was identified as the source of the O2 released from photosynthesis. Robin hill confirmed that energy from light reactions fuels carbon fixation.
Term
What are pigments?
Definition
Molecules that absorb light energy in the visible range.
Term
What is a photon?
Definition
Particle of light that acts as a discrete bundle of energy. The energy content of a photon is inversely proportional to the wavelength of light.
Term
What is the photoelectric effect?
Definition
Removal of an electron from a molecule of light.
Term
The color blue is the _________ amount of energy and red is _________.
Definition
Highest/lowest.
Term
What are the 2 things that happens when a photon strikes a molecule?
Definition
It's energy is either lost as heat or absorbed by the electrons of the molecle which puts said electrons in a higher energy level
Term
What is the absorption spectrum?
Definition
range and efficiency of photons molecule is capable of absorbing
Term
What is chlorophyll a?
Definition
Main pigment in plants and cyanobacteria that is the ONLY pigment that can act directly to convert light energy to chemical energy. It absorbs violet-blue and red light.
Term
What is chlorophyll b?
Definition
Accessory pigment or secondary pigment absorbing light wavelengths that chlorophyll a does not absorb.
Term
What is the structure of chlorophyll?
Definition
Porphyrin ring - complex ring that has alternating double and single bonds that has magnesium in the center of the group. SIMILAR TO HEME
Term
What do photons do the electrons of the porphyrin ring?
Definition
Excite the electrons and are shuttled away from the ring.
Term
What is the action spectrum?
Definition
Relative effectiveness of different wavelengths of light in promoting photosynthesis. Corresponds to the absorption spectrum for chlorophylls
Term
What is carotenoid?
Definition
Can absorb a wide range of energies and scavenge free radicals. It is an antioxidant. Same structure as cholorphyll.
Term
What is phycobiliprotein?
Definition
Important in low-light ocean areas.
Term
What is the antenna complex?
Definition
Hundred of accessory pigment molecules that gather photons and feeds the captured light energy to the reaction center.
Term
What is the reaction center that the antenna complex feeds its gathered photons to?
Definition
Includes 1 or more chlorophyll a molecules and passes the excited electrons out of the photosystem and into the electron acceptor.
Term
What does the energy from the antenna complex cause?
Definition
The energy transferred causes the electron to become excited and is then transferred to the electron acceptor. Water then donates an electron to cholorphyll A to replace the one it just lost to the electron acceptor.
Term
What is the 1st step of the light-dependent reaction?
Definition
1.) Primary photoevent
A photon of light is captured by a pigment molecule
Term
What is the 2nd step of the light-dependent reaction?
Definition
2.) Charge separation
Energy is transferred to the reaction center; an excited electron is transferred to an acceptor molecule
Term
What is the 3rd step of the light-dependent reaction?
Definition
3.) Electron transport
Electrons move through carriers to reduce NADP+
Term
What is the 4th and final step of the light-dependent reaction?
Definition
4.) Chemiosmosis
This is where ATP is produced
Term
What is cyclic photophosphorylation? NO OXYGEN
Definition
Where one photosystem is used. Anoxygenic photosynthesis. The excited electron is passed to the electron transport chain and this generates a proton gradient for ATP synthesis. Once ATP is created, the electron is transferred to another acceptor and then back to the reaction center to start again.
Term
In oxygenic photosynthesis, what are the two photosystems that are connected?
Definition
Photosystem II which can generate an oxidation potential high enough to oxidize water, and Photosystem I which functions somewhat like sulfur bacteria.
Term
What happens when the two photosystems work together?
Definition
They carry out NONCYCLIC transfer of electrons that is used to generate ATP and NADPH.
Term
What is the process of noncyclic oxygenic photosynthesis?
Definition
PHOTOSYSTEM II IS FIRST!
1.) Accessory pigments shuttle energy to the P680 rxn cntr
2.) Excited electrons from P680 are transferred to b6-f
3.) Electrons lost are replaced by an electron released from the splitting of water.
PHOTOSYSTEM I IS SECOND
4.) Receives energy from antenna complex
5.) Energy is shuttled to P700 rxn center
6.) Excited electron is transferred to a membrane-bound electron carrier
7.) Passes electrons to NADP+ to form NADPH
8.) Accepts electrons from plastocyanin to replace those donated to NADP+
Term
What is the b6-f complex?
Definition
Series of electron carriers that are embedded in the thykaloid membrane. Protons are pumped into the thylakoid space to form a proton gradient.
Term
Where is ATP synthase embedded?
Definition
Thylakoid membrane.
Term
How does chemiosmosis occur?
Definition
The protons that were produced during oxygenic photosynthesis are accumulated in the thylakoid space, they move into the stroma ONLY through ATP synthase. ATP is then produced from ADP + P1.
Term
How do cells produce carbohydrates?
Definition
Calvin Cycle.
Term
What energy drives the calvin cycle?
Definition
That obtained from the light-dependent rxns. Can be cyclic or noncyclic. Drives endergonic reaction.
Term
What is the reduction potential?
Definition
a measure of the tendency of a chemical species to acquire electrons and thereby be reduced.
Term
What is the source of protons and energetic electrons?
Definition
NADPH from photosystem 1.
Term
Who is the calvin cycle named after? What is another name for it? Where does it occur? What does it use?
Definition
Melvin Calvin.
Also called C3 Photosynthesis.
Biochemical pathway that allows for carbon fixation.
Occurs in stroma.
Uses NADPH and ATP.
Term
What is Carbon fixation?
Definition
Incorporation of Co2 into organic molecules that occurs in the first step of the calvin cycle.
Term
Ribulose-bis-phosphate (5C) + CO2 -> 2PGA (3C)
Definition
This rxn is catalyzed by ribulose bisphosphate carboxylase/oxygenase AKA RUBISCO.
Term
What are the 3 phases of the Calvin Cycle?
Definition
1.)Carbon Fixation
2.)Reduction
3.)Regeneration of RuBP
Term
What happens in the first phase of the calvin cycle?
Definition
RuBP + CO2 -> PGA
Term
What happens in the 2nd stage of the calvin cycle?
Definition
PGA is reduced (gain electrons) to G3P
Term
What happens in the 3rd stage in the calvin cycle?
Definition
PGA is used to regenerate RuBP.
Term
3 turns around the calvin cycle incorporate enough carbon to produce a new moleucle of ______?
Definition
G3P
Term
6 turns around the calvin cycle incorporate enought carbon to synthesize a new molecule of _______?
Definition
Glucose.
Term
What is the breakdown of energy supplied to the calvin cycle?
Definition
18 ATP and 12 NADPH
Term
Is glucose a direct product of the calvin cycle?
Definition
NO
Term
What is G3P?
Definition
A three carbon sugar used to form sucrose.
Term
What is sucrose?
Definition
A major transport sugar in plants. its a dissacharide made of frutose and glucose.
Term
What else does G3P make?
Definition
Starch - insoluble glucose polymer. Stored for later use.
Term
Photosynthesis uses the products of _______ as starting substrates and respiration uses the products of ______ as its starting substrates.
Definition
Respiration/Photosynthesis
Term
What are the 2 enzymatic activites of Rubisco?
Definition
Carboxylation and Oxidation
Term
What is carboxylation of rubisco?
Definition
Addition of CO2 to RuBP. Favored under normal conditions
Term
What is oxidation of rubisco? (photorespiration)
Definition
Addition of O2 to RuBP. Favored when stomata are closed in hot conditions (low CO2 and high O2)
Term
What competes for the active site on rubisco?
Definition
O2 and CO2
Term
Under hot conditions, leaves lose water by evaporation through openings in the leaves (stomata). The stomata close to conserve water but as a result, O2 builds up inside the leaves and CO2 cannot enter the leaves.
Definition
Conditions favoring photorespiration.
Term
What is C3 photosyntehsis?
Definition
Plants that fix carbon using only the calvin cycle
Term
What is C4 and CAM photosynthesis?
Definition
Add CO2 to PEP to form 4 carbon molecule. Uses the enzyme PEP carboxylase. It has a greater affinity for CO2 so there is no oxygenase activity.
Term
C4 is the ________ solution and CAM is ________ solution.
Definition
Spatial/temporal.
Term
What is the C3 pathway vs C4/CAM?
Definition
C3 - all reactions occur in the mesophyll cells using CO2 that diffuses in through stomata.

C4/CAM - Incorporates CO2 into a 4 carbon molecule of malate in mesophyll cells. Then transported to sheath cells where it is converted back into CO2 and pyruvate.
Term
What does the anatomy of the c4 plant allow?
Definition
Spatial separation of rubisco from o2 production. The calvin cycle and o2 production are separated in space.
Term
What is the benefit of this anatomy? What is the cost?
Definition
Benefit: minimizes photorespiration losses.
Cost: Uses ATP
Term
What are some ex of C4 plants?
Definition
Corn, sugarcane, sorghum, and other grasses.
Term
C4 plant cells initially fix carbon using what?
Definition
PEP carboxylate in mesophyll cells.
Term
What does the carbon fixated to the PEP cbxylse produce? What is that converted to?
Definition
Produces oxaloacetate (4C) converted to malate, then transported to sheath cells.
Term
What happens when the malate is in the sheath cells?
Definition
Decarboxylated to produce pyruvate and CO2.
Term
What then happens to pyruvate and CO2 after they are produced?
Definition
Carbon fixation occurs by rubsico and the calvin cycle (all within the bundle-sheath cells)
Term
In c4 pathway, to produce a single glucose - how many ATP are required?
Definition
An additional 12 versus the calvin cycle alone.
Term
When is c4 respiration advantegous?
Definition
In hot dry weather when photorespiration would remove more than half of the carbon fixed by the usual C3 pathway.
Term
What is a CAM plant?
Definition
Many succulent plants such as cacti, pineapples etc. Stomata open at night and close during the day. Fix CO2 using PEP carboxylase during the night and store in vacuole. Released stored CO2 internally during the day which leads to a high internal co2 concentration. This helps minimize water loss during the day.
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