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chapter 6
growth and growth requirements
26
Microbiology
Undergraduate 2
09/20/2015

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Term
carbon sources
Definition
autotroph- gets it from CO2
heterotroph- catabolize reduced organic material from other organisms
Term
energy sources
Definition
phototroph- energy from sun
chemotroph-energy from redox reactions
Term
when naming an organism based on carbon and energy characteristics how do u do it
Definition
(energy source) +(carbon source) + troph
Term
photoautotroph
Definition
-energy from chemicals, carbon from co2

-plants, algae, cyanobacteria
-green and purple sulfur bacteria use H2S as electron source so they DONT PRODUCE oxygen
Term
chemoautotroph
Definition
-energy from chemicals, carbon from co2
-hydrogen, sulfur, and nitrifying bacteria
Term
photoheterotroph
Definition
-green and purple non sulfur bacteria ( use organic compounds for carbon source) (light for energy)
-archaea
Term
chemoheterotrophs
Definition
-animals, us, molds, protozoa, bacteria, yeasts, fungi (chemical energy source, organic carbon source)
Term
obligate aerobe
Definition
needs oxygen because it is the final electron acceptor
Term
obligate anaerobe
Definition
- can't have oxygen, doesn't have enzyme to get rid of toxic Oxygen forms
Term
facultative anaerobe
Definition
-prefers to use oxygen
-does not need to
Term
aerotolerant anaerobe
Definition
doesn't use oxygen
tolerates its present
Term
toxic forms of oxygen
Definition
1.singlet oxygen O2, polyphenol needed
2. superoxide radical O2, must make superoxide dismutase
3. peroxide anion O22-, must make catalase or peroxidase
4.hydroxyl radical OH- must make antioxidants (melatonin, vitamin E)
Term
there is more ATP with oxygen so
Definition
metabolism and growth is faster
Term
psychrophile
Definition
<0-15 C opt 5, fridge
Term
mesophile
Definition
15-3=45 opt 37, body temp
Term
thermophile
Definition
45-80 opt 70 (can survive 158 F)
Term
neutral pH
Definition
6.5-7.5 best growth
Term
plasmolysiss
Definition
cell loses water and dies in hypotonic environment
Term
synergistic
antagonistic
symbiotic
Definition
antagonistic- microbe attacks harms or kills ex:virus, less resources, more competition

symbiotic- both depend on each other

synergistic- both benefit, but don't depend
Term
steps of biofilm
Definition
1. free microbes are vulnerable to environmental stress and land on surface
2. cells begin making extracellular matrix and secrete quorum sensing molecules (DNA, protein, polysaccharides)
3. quorem sensing triggers cells to change biochemistry and shape
4. new cells arrive, possible including new species, and water channels form in biofilm
5. some microbes may escape
Term
quorum sensing triggers
Definition
previously suppressed genes to change shape, production of enzymes, etc
Term
binary fission
Definition
asexual growth
popuation doubles each time

*generation time= how quickly one binary fission event takes place (avg 20 mins)
Term
closed env
Definition
limited nutrients, waste not diffused away
Term
logarithmic/exponential growth phases
Definition
1.lag phase- adjustment for cells to make enzymes and start reproducing again, fairly short
2. log stage/exponential- cells dramatically increase

INCLOSED ENVIRONMENTS ONLY
3. stationary phase- cells begin to die due to lack of nutrients
4. dead stage/decline stage- cells dying faster than reproducing
Term
our body is an _______ environment
Definition
open- we have a lag phase
Term
which phase is most sensitive to antimicrobial drugs
Definition
log phase
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