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Chapter 6
Microbial Growth
25
Biology
Undergraduate 1
10/06/2011

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Term
What refers to the act of cultivating microorganisms and microorganisms that are cultivated?
Definition
Culture
Term
How are colonies of bacteria characterized?
Definition
Phenotypic: Shape, margin, elevation, size, texture, apperance, pigmentation and optical property.
Term
What term means to transfer a sample of microoranisms into a culture medium?
Definition
Inoculum
Term
What are two sources of inoculum?
Definition
Environmental and Clinical
Term
What are examples of environmental specimen?
Definition
water, soil, and air
Term
What are examples of clinical specimen?
Definition
feces, saliva, blood, cerebrospinal fluid, urine
Term
What are the two forms of culture media?
Definition
liquid and solid
Term
What is liquid media called?
Definition
Term
What are examples of solid culture media?
Definition
agar plate, slant and deep
Term
Broth is made up of what?
Definition
Peptone 3 and beef extract
Term
Agar medium is made up of what?
Definition
Peptone 3, beef extract and agar
Term
When the exact formula of a medium is know it is called ______.
Definition
Defined Medium
Term
When the chemical formula is unknown for a medium it is called _____.
Definition
Complex
Term
Defined medium is also called?
Definition
synthetic
Term
Culture medium is based on four different kind of functions. What are they?
Definition
Selective
Differential
Anaerobic media
Transport media
Term
MacConkey Agar inhibits ___ and doesn't affect ____.
Definition
G pos/G neg
Term
Bile Salts and salt ex. crystal violet are examples of what function of culture medium?
Definition
selective
Term
What function of culture medium is this? favors one and inhibits another
Definition
selective
Term
What function of culture media is this?
Both can grow, but can differentiate
Definition
differential
Term
Which culture media function can grow anaerobic bacteria?
Definition
anaerobic media
Term
What type of culture medium is MacConkey agar based on function?
Definition
selective and differential
Term
Blood agar is what type of culture media based on function?
Definition
differential culture medium
Term
What culture medium can can support the growth of gram neg and gram pos?
Definition
blood agar
Term
What method of obtaining pure cultures is used to separate the cells.
Definition
streak plating
Term
Pure cultures contain how many types of bacteria?
Definition
one
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