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| Mineral that is necessary for some enzymes to become activated. |
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| A substance that significantly changes the environment in which an enzyme is active, causing it to cease activity |
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| Decreases the activation energy for a reaction |
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| The substance formed during a chemical reaction. |
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| What is the primary generic equation used to calculate the enzyme concentration? (as instructed in lab materials) |
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| When enzymes are being produced a specific sequence of ___ is assembled at the ribosomes in the cytosol |
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| ___ are molecules that are acted upon by enzymes |
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| Enzymes ___ chemical reactions |
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| Enzymes belong to the ___ class of biomolecules |
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| The more enzymes there are, the faster the rate of ractions will take place, and the more ___ will be formed. |
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| the enzyme pepsin is secreted in the stomach where it begins to break down proteins. what would you expect to happen to the effectiveness of pepsin as it moves into the small intestine where the base bicarbonate is secreted? |
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| Pepsin's activity will decrease. This enzyme is secreted into the stomach which has a highly acidic environment. As it enters a more basic environment, the enzyme will move out of its optimum pH range (pH will increase), which will slow the rate of activity of this enzyme. |
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| After running the enzyme assay, the solution was put into an instrument called a |
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| After stopping the reaction, the amount of ___ was measured in the solution. |
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| After stopping the reaction, the amount of ___ was measured in the solution. – product, After the reaction was stopped and the cuvette was placed into the instrument, this unitless measurement was recorded. – absorbance |
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| three major environmental factors that affect enzyme activity |
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| pH, temperature, Substrate concentration |
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| when an enzyme is exposed to more and more substrate, its rate of activity will increase proportionally. this will continue until the enzyme is working as fast as possible- no matter how much more substrate is added. it cant act any faster. the enzyme has reached a state of |
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| can involve putting molecules together |
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| can involve breaking molecules down into smaller parts |
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| the amount of energy required to initiate a chemical reaction |
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| proceed slowly or not at all |
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| there will be a transfer of a phosphate in the reaction |
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| there will be a removal of a phosphatase group in the reaction |
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| there will be removal of hydrogen(from the substrate) in the reaction |
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| there will be a change in a substrate structure, but not in its make-up |
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