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Definition
| Know EXACT contents of media |
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| Exact contents are not known. Ex. V8 juice |
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| Liquid Media, used to grow large colonies of Microbes |
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| Gelatin type media called Agar is used. Made to grow discrete colonies on it's surface |
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Definition
| A small amount of Agar is used. Used to see if microbes are motile |
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Definition
| Exactly that. A general purpose media that grows most microbes |
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Definition
| Has ingredients that will allow some microbes to grow while others not to grow |
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Definition
| Allows you to tell one microbe from another |
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Definition
| Addint additional nutrients for microbes that won't grow ona General Media |
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Definition
| Several Microbes ina sample |
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| Microbes introduced that shouldn't be present. |
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Term
| Describe the streak plate method |
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Definition
| used to isolate microbes into pure culture by spreading teh microbe across a Petri dish in three or four overlapping areas |
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Term
| Describe Serial Dilution Technique |
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Definition
| Take a known volume of inoculum from one test tube to the next and then spreading known volumes from each tube onto Petri dishes |
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Term
| Streak plate is used to do what? |
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Definition
| cut down the total amounts of all Microbes |
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Term
| Serial Dilution is used for what? |
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Definition
| Cut down the number of diffrent microbes |
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Term
| Nutrient Agar is what type of media?? |
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Definition
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Term
| Green Algae Media is what type of media? |
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Definition
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Term
| mannitol Salt Agar is what? |
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Definition
| Differential and Selective |
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| Eosin Methylene Blue medium |
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| Best used for slides that have been stained |
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Definition
| Best used for living cells |
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Definition
| Best when looking at living cells |
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Term
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Definition
| Uses a diffrent light and the object can be injected with dyes that attach to diffrent things like protiens or starches |
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Term
| Compound light microscopes |
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Definition
| Uses light to highlight or silouette the objects |
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Term
| Fluorescent light microscopes |
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Definition
| Similar to compound light microscopes but uses a diffrent light that can pick up diffrent dyes that are inserted |
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Term
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Definition
| Will show much smaller objects than the compound light |
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Term
| Scanning electron microscope |
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Definition
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| Transmission electron microscope |
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Definition
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Term
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Definition
| Stabilizing the internal structure of the cell |
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Definition
| Cut into small sections or smeared |
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Definition
| Everything dyed the same color |
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Definition
| Stains the background and not the object |
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Definition
| Will stain diffrent cellular structures |
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Term
| What cellular structures do you see with a light microscope? |
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Definition
| Whole cells, large organelles, and bacteria |
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Term
| What additional structures can you see with an electron microscope? |
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Definition
| Phospholipid bilayer, all organelles, ribosomes, viruses, and DNA |
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Term
| Stains used in Gram Stain?? |
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Definition
| Chrystal Blue and Safranin |
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