Shared Flashcard Set

Details

Chapter 3
questions
45
Biology
Undergraduate 2
01/31/2011

Additional Biology Flashcards

 


 

Cards

Term
Chemically Defined media
Definition
Know EXACT contents of media
Term
Complex Media
Definition
Exact contents are not known. Ex. V8 juice
Term
Broth Media
Definition
Liquid Media, used to grow large colonies of Microbes
Term
Solid Media
Definition
Gelatin type media called Agar is used. Made to grow discrete colonies on it's surface
Term
Semisolid media
Definition
A small amount of Agar is used. Used to see if microbes are motile
Term
General purpose media
Definition
Exactly that. A general purpose media that grows most microbes
Term
Anaerobic media
Definition
No oxygen used to grow
Term
Selective Media
Definition
Has ingredients that will allow some microbes to grow while others not to grow
Term
Differential Media
Definition
Allows you to tell one microbe from another
Term
Enrichment media
Definition
Addint additional nutrients for microbes that won't grow ona General Media
Term
Mixed Media
Definition
Several Microbes ina sample
Term
Pure culture
Definition
One Microbe isolated
Term
Contaminated Culture
Definition
Microbes introduced that shouldn't be present.
Term
Describe the streak plate method
Definition
used to isolate microbes into pure culture by spreading teh microbe across a Petri dish in three or four overlapping areas
Term
Describe Serial Dilution Technique
Definition
Take a known volume of inoculum from one test tube to the next and then spreading known volumes from each tube onto Petri dishes
Term
Streak plate is used to do what?
Definition
cut down the total amounts of all Microbes
Term
Serial Dilution is used for what?
Definition
Cut down the number of diffrent microbes
Term
Nutrient Agar is what type of media??
Definition
General Purpose
Term
Green Algae Media is what type of media?
Definition
Chemically
Term
mannitol Salt Agar is what?
Definition
Differential and Selective
Term
Flo Agar is
Definition
Differential
Term
Blood Agar is
Definition
Differential, enriched
Term
Eosin Methylene Blue medium
Definition
Selective
Term
Rabbit Dung Agar
Definition
Complex
Term
Potato Dextrose Agar
Definition
General purpose
Term
milk agar
Definition
Complex
Term
Brightfeild
Definition
Best used for slides that have been stained
Term
Dark field
Definition
Best used for living cells
Term
Phase Contrast
Definition
Best when looking at living cells
Term
Fluorescent
Definition
Uses a diffrent light and the object can be injected with dyes that attach to diffrent things like protiens or starches
Term
Compound light microscopes
Definition
Uses light to highlight or silouette the objects
Term
Fluorescent light microscopes
Definition
Similar to compound light microscopes but uses a diffrent light that can pick up diffrent dyes that are inserted
Term
Electron microscope
Definition
Will show much smaller objects than the compound light
Term
Scanning electron microscope
Definition
3D image
Term
Transmission electron microscope
Definition
2D
Term
Fixing
Definition
Stabilizing the internal structure of the cell
Term
Sectioning
Definition
Cut into small sections or smeared
Term
Simple stain
Definition
Everything dyed the same color
Term
Negative Stain
Definition
Stains the background and not the object
Term
Differential stain
Definition
Will stain diffrent cellular structures
Term
What cellular structures do you see with a light microscope?
Definition
Whole cells, large organelles, and bacteria
Term
What additional structures can you see with an electron microscope?
Definition
Phospholipid bilayer, all organelles, ribosomes, viruses, and DNA
Term
Stains used in Gram Stain??
Definition
Chrystal Blue and Safranin
Term
Color is Gram +
Definition
Purple
Term
Color is Gram -
Definition
Pink
Supporting users have an ad free experience!