Term
| A microscope with only one lens |
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Definition
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Term
| Microscope where the objective lens is magnified again by the ocular lens |
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Definition
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Term
| What is the total magnification? |
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Definition
| objective lens + ocular lens power |
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Term
| The ability of the lense to distinguish two points |
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Definition
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Term
T/ F? Shorter wavelengths of light provide greater resolution |
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Definition
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Term
| The light bending ability of a medium |
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Definition
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Term
| What is used to keep the light from bending? |
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Definition
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Term
| Type of microscope that views dark objects against a bright background. Light reflected off of the speciment does not enter the objective either. |
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Definition
| Brightfield illumination microscope |
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Term
| Microscope that light objects are viewed against a dark background. Light reflected off the specimen enters through the objective lens |
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Definition
| Darkfield illumination microscope |
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Term
| Microscopy that accentuates diffraction of the light that passes through a specimen |
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Definition
| Phase-contrast microscopy |
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Term
| Microscopy that accentuates diffraction of the light that passes through a speciment and uses 2 beams of light |
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Definition
| Differential interference contrast microscopy |
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Term
| Microscopy that involves using UV lights. The substance absorbs UV lights + emits visible light. |
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Definition
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Term
| Microscopy that involves the use of fluorochromes and a laser light. The laser light illuminates each plane in a specimen to produce a 3d image |
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Definition
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Term
| Type of microscopy that measures sound waves that are reflected back from an object. Used to study cells attached to a surface. |
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Definition
| Scanning Acoustic Microscopy |
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Term
| Microscopy that involves the use of electrons instead of lights. |
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Definition
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Term
| Type of microscopy that uses ultra thin sections of specimens. Light passes through specimen, then an electromagnetic lens, to a screen or film |
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Definition
| Transmission Electron Microscopy |
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Term
| A microscopy that uses an electron gun produces a beam of electrons that scans the surface of a whole specimen. |
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Definition
| Scanning electron microscopy |
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Term
microscopy uses a metal probe to scan a specimen |
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Definition
| Scanning Probe Microscopy |
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Term
| Uses a metal and diamond probe inserted into the specimen |
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Definition
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Term
| A thin film of a solution of microbes on a slide |
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Definition
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Term
Whats the purpose of fixing a smear? |
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Definition
To attach the microbes to the slide and to kill the microbes |
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Term
| In a basic dye, the chromophore is _______ |
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Definition
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Term
In an acidic dye, the chromophore is a _______ |
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Definition
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Term
What is negative staining? |
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Definition
| Staining the background instead of the cell |
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Term
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Definition
use of a single basic dye |
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Term
| Whats the purpose of a mordant? |
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Definition
| It is used to hold in a stain or make the specimen larger |
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Term
| The gram stain classifies the stains into 2 results |
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Definition
gram positive gram negative |
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Term
| Which gram result bacteria tend to be killed by penicillin |
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Definition
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Term
Which gram result bacteria are more resistant to antibiotics |
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Definition
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Term
| What are the 3 common microbial shapes? |
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Definition
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Term
Type of stain that tests for cells that retain a basic stain in the presence of acid-alcohol |
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Definition
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Term
| What occurs in non-acid fast cells? |
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Definition
| They lose the basic stain when rinsed with acid alcohol and are counterstained with a different color to see the difference. |
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Term
| What is negative staining useful for? |
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Definition
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Term
| What is required to drive a stain into endospores? |
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Definition
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Term
| What does flagella stain require to make the flagella wide enough to see? |
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Definition
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