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Chapter 20
Carbohydrate Biosynthesis in Plants & Bacteria
28
Biochemistry
Undergraduate 3
02/16/2016

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Term
CO2 assimilation
Definition
the conversion of CO2 to simple organic compounds which is catalyzed by enzymatic machinery found in the chloroplasts of green plants
Term
CO2 fixation (carbon fixation)
Definition
the specific reaction in which CO2 is incorporated into a three-carbon organic compound (the triose phosphate 3-phosphoglycerate)
Term
plastids
Definition
where most of the biosynthetic activities in plants occur; a family of self-reproducing organelles bounded by a double membrane & containing a small genome that encodes some of their proteins
Term
chloroplasts
Definition
the sites of CO2 assimilation
Term
amyloplasts
Definition
colorless plastids
Term
How can chloroplasts by converted to proplastids?
Definition
by the loss of their internal membranes & chlorophyll; proplastids are interconvertible with amyloplasts
Term
carbon-fixation reaction
Definition
the first stage in the assimilation of CO2 into biomolecules; condensation of CO2 with a five-carbon acceptor (ribulose 1,5-biphosphate) to form two molecules of 3-phospho-glycerate
Term
3 stages of carbon dioxide assimilation
Definition
1. fixation of CO2 into 3-phosphoglycerate
2. conversion of 3-phosphoglycerate to glyceraldehyde 3-phosphate
3. regeneration of ribulose 1,5-biphosphate from triose phosphates
Term
Where is the 14C predominantly located in 3-phosphoglycerate?
Definition
in the carboxyl carbon atom
Term
C3 plants
Definition
plants in which 3-phosphoglycerate is the first intermediate in carbon dioxide assimilation
Term
ribulose 1,5-biphosphate carboxylase/oxygenase (rubisco)
Definition
the enzyme that catalyzes incorporation of CO2 into an organic form
Term
rubisco activase
Definition
overcomes inhibition by promoting ATP-dependent release of the ribulose 1,5-biphosphate, exposing the Lys amino group to nonenzymatic carbamoylation by CO2; followed by Mg2+ binding, this activates rubisco
Term
3-phospho-glycerate kinase
Definition
a stromal molecule which catalyzes the transfer of a phosphoryl group from ATP to 3-phosphoglycerate to yield 1,3-biphosphoglycerate
Term
glyceraldehyde 3-phosphate dehydrogenase
Definition
the chloroplast-specific isozyme that catalyzes NADPH donating electrons in a reduction to produce glyceraldehyde 3-phosphate & Pi
Term
What is the prosthetic group of transketolase & what does it require?
Definition
prosthetic group = thiamine pyrophosphate

requires = Mg2+
Term
sedoheptulose 1,7-biphosphate
Definition
a seven-carbon molecule formed by aldolase combining erythrose 4-phosphate with dihydroxyacetone phosphate
Term
ribulose 5-phosphate
Definition
what the pentose phosphates formed in the transketolase reactions (ribose 5-phosphate & xylulose 5-phosphate) are converted to; it is phosphorylated to ribulose 1,5-biphosphate by ribulose 5-phosphate kinase
Term
ferredoxin-thioredoxin reductase
Definition
catalyzes the reaction in which electrons (with illumination) flow from photosystem I to ferredoxin which passes electrons to a small, soluble, disulfide-containing protein called "thioredoxin"
Term
mitochondrial respiration
Definition
carried out by plants in the dark; the oxidation of substrates to CO2 & the conversion of O2 to H2O
Term
What causes photorespiration to occur?
Definition
the lack of specificity of the enzyme rubisco
Term
What causes the creation of metabolically useless 2-phosphoglycolate?
Definition
molecular oxygen competes with CO2 at the active site & about once in every three or four turnovers, rubisco catalyzes the condensation of O2 with ribulose 1,5-biphosphate to form 3-phosphoglycerate & 2-phosphoglycolate
Term
glycolate pathway
Definition
converts 2 molecules of 2-phosphoglycolate to a molecule of serine & a molecule of CO2
Term
glycine decarboxylase complex
Definition
oxidizes glycine to CO2 & NH3 with the concomitant reduction of NAD+ to NADH & transfer of the remaining carbon from glycine to the cofactor tetrahydrofolate
Term
photorespiration
Definition
the combined activity of the rubisco oxygenase & the glycolate salvage pathway to consume O2 & produce CO2
Term
C3 plants
Definition
plants which use the carbon-assimilation method in which the first step is a reaction of CO2 with ribulose 1,5-biphsophate to form 3-phosphoglycerate
Term
Where does the reaction catalyzed by phosphoenolpyruvate carboxylase which uses HCO3- as the substrate (not CO2)?
Definition
in the cytosol of leaf mesophyll cells
Term
malic enzyme
Definition
allows for malate to be oxidized & decarboxylated to yield pyruvate & CO2 in bundle-sheath cells; NADP+ is reduced
Term
pyruvate phosphate dikinase
Definition
catalyzes the enzymatic reaction in which the pyruvate (formed by decarboxylation of malate in bundle-sheath cells) is transferred back to the mesophyll cells & converted to PEP
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