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| cells from one strain produced a heritable change in another strain |
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| Hershey- Chase experiment DNA was labeled with |
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| Hereshey-Chase experiment proteins labeled with |
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| genetic transformation of eukaryotic cells |
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| a gene that confers an observable phenotype |
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| found the structure of DNA using x-ray crystallography |
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| deoxyribose, phosphate group, nitrogen containing base |
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| the number of purines = the number of pyrimidines |
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| built the DNA structure model |
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| polynucleotide chains ran in opposite directions |
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-double stranded helix w/ uniform diameter -right handed -antiparallel -nitrogenous bases are exposed in major and minor grooves |
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| has a free phosphate group |
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| has a free hydroxyl group |
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-store genetic info -genetic material able to mutate -genetic material is replicated in cell division -genetic material expressed as phenotype |
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| T or F Dna serves as a template for synthesis of DNA |
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| parent serves as a template and new molecules have one old and one new strand |
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| original helix only serves as a template |
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| parent fragments serve as templates, assembling old and new parts into molecules |
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| join the nucleotides added to the new strand |
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| helps increase DNA replication |
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| starts DNA replication and is made up of RNA |
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| adds nucleotides to the 3 prime end |
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| is the site where DNA unwinds to expose bases |
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| uses energy from ATP hydrolysis to unwind the DNA |
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| single-stranded binding proteins |
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| keep the strands from getting back together |
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| is oriented to grow at its 3 prime end as the fork opens |
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| oriented so the 3 prime end gets farther from the fork |
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| small discontinuous stretches of the lagging strands |
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| adds nucleotides to the 3 prime end |
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| replaces the primer with DNA |
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| catalyzes the phosphodiester linkages |
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| catalyze many polymerizations each time they bind to DNA |
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| separates the two chromosomes |
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| T or F linear DNA have one origin of replication |
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| repetitive sequences at the end of eukaryotic chromosomes |
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| catalyzes addition of lost telomeric DNA sequences |
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| if bases are paired incorrectly, the nucleotide is removed |
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| detects mismatched bases on new strands that have not yet been modified |
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| enzymes constantly scan DNA for damaged bases, damaged bases are excised and DNA polymerase I adds correct ones |
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| polymerase chain reaction (PCR) |
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Definition
| make copies of DNA sequences in a cyclical process |
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| separates the DNA into 2 strands |
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