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Ch17
quiz
19
Biology
Undergraduate 2
03/19/2013

Additional Biology Flashcards

 


 

Cards

Term
Reverse transcriptase is an enzyme normally used by RNA viruses to make DNA from their RNA; researchers use the enzyme to make cDNA from the RNA produced by cells
Definition
True
Term
DNA fingerprints are based on the occurrence of variable-sized fragments of DNA (tandem repeats) in each individual
Definition
True
Term
Because DNA works by the same general principles in all organisms, it is possible to isolate a gene from one species, and artificially place it into another
Definition
True
Term

Which of the following is required for PCR?

All of these

Short "primers" from each end of the target DNA

DNA region to be amplified

Nucleotides (A,C,G, and T)

DNA polymerase

Definition
All of these
Term
A cDNA library contains only the portion of the DNA that is transcribed into RNA
Definition
True
Term
A laboratory method used to generate a large number of copies of a particular segment of DNA is called:
Definition
Polymerase Chain Reaction (PCR)
Term
A plasmid is a small, circular, extra-chromosomal piece of DNA that occurs in many bacteria
Definition
True
Term

Which of the following is a transgenic experiment that has been successfully carried out?

Salmon containing an antifreeze gene.

Pigs that contain human antithrombi III.

Goats that produce milk containing spider silk.

Rabbits containing the human interleukin-2 gene

All of these.

Definition
All of these
Term
A "cloning vector" is the name given to researchers who take DNA from one species and place it into another
Definition
False
Term
DNA fingerprinting is done by generating fragments of DNA using restriction enzymes; the resulting fragments may show Restriction Fragment Length Polymorphisms (RFLP's)
Definition
True
Term
Automated DNA sequencing may be used to determine the nucleotide sequence of a portion of isolated DNA
Definition
True
Term
The transfer of genes from one species to another can only be done in a laboratory. There are no known cases of genes moving from one species to another in nature
Definition
False
Term
When cloning a gene in bacteria, which of the following is the usual location of insertion of the new DNA?
Definition
Bacterial plasmid
Term
Restriction enzymes are enzymes that naturally occur in your body, which break up pieces of DNA from bacteria
Definition
False
Term
The transfer of genes from one species to another can only be done in a laboratory. There are no known cases of genes moving from one species to another in nature
Definition
False
Term
To find a particular gene of interest in a gene library, researchers use a short fragment of DNA that is complementary to the gene. This fragment is called a:
Definition
 
Term
PCR (Polymerase Chain Reaction) is a method used by living cells to make a large number of copies of a protein
Definition
False
Term
A collection of host cells (usually bacteria) that each contain fragments of an entire genome is called:
Definition
A genomic library
Term
When cloning a gene in bacteria, which of the following is the usual location of insertion of the new DNA?
Definition
Bacterial plasmid
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