Term
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Definition
| The process in which existing DNA strands are used to make new DNA strands |
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Term
| When does DNA replication occur? |
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Definition
| when the strands of DNA unwind and each strand is used as a template to make a new strand according to the AT/CG rule. The resulting DNA molecules have the same base sequence as the original DNA. |
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Term
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Definition
| By labeling DNA with heavy and light isotopes of nitrogen and using centerfugation, they showed that DNA replication is semiconservitive. |
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Term
| Bacterial DNA Replication |
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Definition
| Begins at a single origin of replication and proceeds bidirectionally around the circular chromosome. |
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Term
| Bacterial DNA Replication Initiation |
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Definition
| DNA replication is initiated when DnaA proteins bind to five DnaA boxes at the origin of replication and cause the AT-rich region to unwind. |
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Term
| DNA Polymerase III (in E.Coli) |
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Definition
| is an enzyme with several subunits that wraps around the DNA like a hand. |
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Term
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Definition
| Need a primer to synthesize DNA and make new DNA strands in a 5' to 3' direction. |
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Term
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Definition
| The leading strand is made continuously in the direction of the replication fork and the lagging strand is made of Okazaki fragments in the direction away from the fork. |
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Term
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Definition
| A complex between helicase and primase |
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Term
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Definition
| Complex between the primosome and dimetric DNA polymerase |
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Term
| High Fidelity of DNA Replication |
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Definition
| this is a result of (1) the stability of hydrogen bonding between the correct bases, (2) the phenomenon of induced fit, and (3) the proofreading ability of DNA polymerase. |
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Term
| Regulation of Bacterial DNA Replication |
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Definition
| Regulated by the amount of DnaA protein and by the methylation of GATC sites in oriC |
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Term
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Definition
| He devised a method to measure DNA replication in vitro |
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Term
| How Proteins Involved with DNA Replication were ID'd |
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Definition
| the isolation and charactorization of temperature- sensitive mutants |
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Term
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Definition
| Eukaryotic chromosomes contain multiple origins of replication. |
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Term
| Origin of Replication Complex |
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Definition
| A group of 6 proteins made up of part of the prereplication complex |
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Term
| DNA Replication Licensing |
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Definition
| A process that is completed by the binding of MCM helicase |
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Term
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Definition
| An enzyme that removes RNA primers from Okazaki fragments |
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Term
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Definition
| Uses a short RNA molecule as a template to add repete sequences to telomeres. |
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