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Definition
| The spectrum formed by electromagnetic radiation that has passed through a medium in which radiation of certain frequencies is absorbed. |
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Term
1. As a method for characterization and identification of a compound (instrument used as a spectrophotometer)
2. As a method for determining the concentration of a compound in solution (instrument used as colorimeter) |
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Definition
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Term
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Definition
| (Optical density per unit concentration) relates the concentration of the substance being measured (in moles) to the absorbance of the substance in solution (how well the substance in solution blocks light beamed through it from getting out on the other side). |
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Term
--> Purine, Pyrimidine, Amino acids
DNA absorption at 260 nm
Protein absorption at 280 nm (tryptophan, histadine, tyrosine) |
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Definition
| ______ and ______ bases are stronger absorbers per residue than the ______. |
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| Equation of Optical Density |
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Definition
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| Number, molecules, absorbing substance |
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Definition
| In Solutions, OD is proportional to the ____ of _______ of _______ _______ per unit volume of solution. |
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Definition
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Definition
| This law states that there is a linear relationship between concentration and __________. |
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Definition
| can be used to obtain the concentration of Bromophenol Blue or protein in solutions of unknown concentration. |
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Definition
| A serial dilution is the stepwise dilution of a substance in solution. |
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