Term
In project 2 we looked at data showing actin localization around the cortex of the cell.
We did this to evaluate:
a) chromosome movement during cytokinesis
b) cell membrane relaxation at the poles
c) cell membrane contraction at the poles
d) chromosome movement during anaphase |
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Definition
| b) cell membrane relaxation at the poles |
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Term
There are three types of microtubules that help with mitosis, kinetochore, astral, and interpolar. How do they differ?
a) only the interpolar microtubules pull (and push) chromosomes
b) only kinetochore microtubules are made of alpha-beta dimers strong (and the wound) together
c) only the kinetochore microtubules pull (and push) chromosomes
d) only kinetochore microtubules have plus and minus ends directing the flow of motor proteins |
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Definition
| c) only the kinetochore microtubules pull (and push) chromosomes |
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Term
Actin binding proteins have many roles. Which list offers important distinctions between two actin binding proteins?
a) formin helps actin polymers become capped while fimbrin helps actin polymers remove their caps
b) ARP2/3 is a motor protein on branched actin polymers, myosin is a motor protein on unbranched actin polymers
c) myosin is a motor protein that tracks along actin polymers while ERM are proteins that help attach actin to the plasma membrane
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Definition
| c) myosin is a motor protein that tracks along actin polymers while ERM are proteins that help attach actin to the plasma membrane |
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Term
Which of the statements below accurately offers a distinction between actin and tubulin?
a) tubulin polymers have two different motor proteins but actin polymers only have one
b) actin polymers can join at branches (only) while tubulin polymers join at branches and parallel bundles
c) tubulin polymers are more unstable than actin polymers
d) tubulin is used in kinetochore microtubules but actin is used in astral microtubules |
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Definition
| a) tubulin polymers have two different motor proteins but actin polymers only have one |
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Term
The relaxation of the cortex at the poles during cell division is probably a new concept to you. In the data we examined from the paper, you learned about some genes (and the proteins they make) that help to regulate this process. Which statement below accurately reflects the genetic control of pole relaxation?
a) blue lasar activation of PP1 leads to blebbing which is a sign of membrane relaxation
b) blue lasaar light activation of Sds22 leads to blebbing which is a sign of membrane relaxation
c) microtubule poisons result in inhibition of relaxation |
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Definition
| b) blue lasaar light activation of Sds22 leads to blebbing which is a sign of membrane relaxation |
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Term
How is myosin INactivated?
[image]
a) through Rho Kinase
b) by CDK1
c) by PP1
d) through myosin phosphatase |
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Definition
| d) through myosin phosphatase |
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Term
What is the "Y axis" in panel b?
[image]
a) time (seconds)
b) intensity of staining
c) position along the radius of the cell |
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Definition
| c) position along the radius of the cell |
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Term
B and H show kymographs of cells expressing Myosin::GFP. Kymographs were derived from a line along the apical-basal axis (apical is on top, basal on the bottom). C & I are intensity measurements performed on the kymographs. Compare B & C (wild type) with H & I (mutant causing Pins to be localized all over the cortex). NEB = Nuclear envelope breakdown. AO = anaphase onset. Which statements are correct? Check all that apply.
[image]
[image]
a) Pins is sufficient to induce uniform Myosin enrichment just after NEB
b) Uniform cortical Pins localization shortens the time between NEB and AO
c) myosin intensity is always the same
d) myosin intensity is generally higher on the apical cortex in wild type cells |
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Definition
| a) Pins is sufficient to induce uniform Myosin enrichment just after NEB |
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Term
How do you interpret this data. Check all that apply.
[image]
a) wild type centrosomes are asymmetric
b) centrioles are missing in bld10 mutants, that is why both centrosomes contain microtubules
c) Bld10 is required for nucleating microtubules on both centrosomes
d) Bld10 is not required for the formation of centrioles |
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Definition
| d) Bld10 is not required for the formation of centrioles |
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Term
Where is the protein shown in green below localized? Check the best answer.
[image]
a) impossible to tell
b) neither apical nor basal
c) basal |
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Definition
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