Term
Fill in the chart
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Definition
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Definition
| reporter gene intruduced with retrovirus that targets dividing cells, transgene inherited by all progeny[image] |
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Term
| fusing an M-phase cell with a cell in a different phase makes the second cell enter M-phase due to _____ |
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Definition
| mitosis promoting factor (MPF) |
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Term
| MPF correlates with expression of what other protein? |
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Definition
| cyclin (does not initiate M-phase, but is required)[image] |
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| How are MPF an cyclin related? |
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Definition
MPF contains a cdk, which requires binding to cyclin
(activity regulated by phosphorylation) |
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Definition
| phosphorylates many cell components involved in M phase when active e.g. nuclear lamin -->breakdown of membrane |
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Definition
| Destroyed by proteases at metaphase-anaphase transition (via ubiquitin targeting) |
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| Describe the yeast cell cycle. |
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Definition
| they have only one kinase that regulates S- and M-phase entry, but use different cyclins for each. The cyclin determines the kinase's substrates |
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Term
| Contrast vertebrate cell cycle with yeast cell cycle |
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Definition
| vertebrates have multiple kinases and cyclins regulating each step of the cell cycle |
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Term
| What is the function of tumor suppressor genes, such as Rb? |
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Definition
| to keep cells from dividing (keep in G1/G0) |
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| In what way does Rb function? |
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Definition
stops S phase entry by inhibiting E2F
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Definition
| needed by most cell types to divide, neural progenitors usually divide in response to EGF or FGF (fibroblast growth factor) |
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Term
| growth factor functioning |
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Definition
| activate specific receptors, many of which are RTKs (receptor tyrosine kinases) |
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Term
| what happens in response to ligand binding to growth factor receptors? |
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Definition
MAPK pathway activated, make cyclin D, deactivates Rb
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Definition
cell surface receptor that promotes cell division, expressed by neural progenitor cells, Delta and Jagged ligands
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Term
| Describe positional influences of cell division |
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Definition
- alar+basal: more division than roof+floor plates
- differences along anerior-posterior length
- position-specific transcription factors likely control this
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