Term
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Definition
| These are small single celled organisms |
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Term
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Definition
| Sometimes motile using flagells |
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Term
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Definition
| NO membrane bound organelles |
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Definition
| Small Circular DNA as their genome |
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Term
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Definition
| Have membrane bound organelles |
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Term
| Nucleus, Endoplasmic Reticulum, Golgi Apparatus, Mitochondria, Vacuoles, Chloroplasts(sometimes) |
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Definition
| What do Eukaryotes have that Prokaryotes don't have? |
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Term
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Definition
| What is the site of Fatty acid and phospholipid synthesis? |
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Term
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Definition
| Proteins are translated on ___ Ribosome complexes. |
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Term
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Definition
| site of synthesis of membrane and organelle proteins and all secreted proteins |
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Term
| nucleolus, rRNA, ribosome |
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Definition
| The nucleus contains ________ where _______ synthesis and processing occurs and ________ subunits are assembled. |
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Term
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Definition
| The Golgi apparatus has stacks of flattened, interconnected membrane bound compartments called ________ |
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Term
| mitochondria and chloroplasts |
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Definition
| 2 organelles that contains its own DNA and 70s ribosomes |
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Term
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Definition
| Site of Electron Transport |
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Term
| Oxidative Phosphorylation, ATP |
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Definition
| Proton Gradient is used during __________ _______________ producing most of the ________ for the cell |
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Term
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Definition
| site of light and dark reactions, carbon fixation. |
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Term
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Definition
| Stacks of thylakoid membranes |
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Term
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Definition
| stores water, ions, and nutrients; degrades macromolecules and functions in cell elongation |
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Term
| contractile, cytosol, plasma membrane |
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Definition
| A _____________ vacuole takes up water from the ___________ and periodically discharges its contents by fusion with the __________ ____________ |
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Term
| staining, methylene blue, acidic |
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Definition
| Without _________, a cell will appear ghostlike. ___________ __________ is a stain that stains __________ molecules |
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Term
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Definition
| ____________ staining allows for the identification of certain organelles |
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Term
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Definition
| ____________ stains are permeant dyes that can stain cells without killing them. |
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Term
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Definition
| Vital stains can become ________ in specific compartments which can be identified due to their differential absorption of light |
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Term
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Definition
| rod-shaped prokaryotic bacteria |
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Term
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Definition
| blue-green prokaryotic algae, can photosynthesize and has gliding motion |
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Term
| Squamosal epithelial cells |
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Definition
| found in the cheek of the mouth |
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Term
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Definition
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Term
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Definition
| A eukaryotic, filamentous green alga and have spiral shaped chloroplasts |
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Term
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Definition
| protist that is motile via cilia |
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Term
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Definition
| ___________ contains unusual organelles located beneath the pellicle called ___________ |
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Term
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Definition
| A _________ ________is used to trigger trichocysts |
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Term
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Definition
| __________ are fresh water ciliates that are about __ microns long |
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Term
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Definition
| ________'s ________ is a stain for carbohydrates |
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Term
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Definition
| Used for fixation of cells |
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Term
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Definition
| A prominent internal structure in Tetrahymena |
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Term
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Definition
| India Ink is an emulsion of _____________ particles |
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Term
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Definition
| _______ _____ is an emulsion of Carbon particles |
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Term
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Definition
| ___________ ______ is a pH indicating dye that is red below pH 6.8 and yellow above pH 8.0 |
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Term
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Definition
| __________ ______ is a pH indicating dye that is blue below pH 3.0 and red above pH 5 |
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Term
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Definition
| At pH 4.2, ______ _____ is a DNA specific stain that stains DNA a bluish green color |
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Term
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Definition
| ________ __ stains the cytoplasm of the cell pink due tot he high concentration of ribosomes found in the cytoplasm |
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Term
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Definition
| A mix of _________ ________ and _________ __ will simultaneously stain the nucleus green and the cytoplasm pink |
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Term
| Janus Green B, mitochondria |
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Definition
| Cytoplasmic bodies that stain with ____ _____ _ under aerobic conditions, but lose this staining under anaerobic conditions, are called ______________ |
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Term
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Definition
| Chromosomes appear in cell cycle during _________ |
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Term
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Definition
| DNA aligns on _________ plate and each of the 2 chromatids now has a specialized structure called a _____________. |
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Term
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Definition
| The kinetochore is located at the ___________ region |
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Term
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Definition
| During _____ centromeres separate and chromosomes are pulled to opposite poles as kinetichjore mictrotubules shorten |
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Term
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Definition
| ___________, or division of the cytoplasm, occurs at the end of ____________ |
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Term
| Toludine Blue, polysaccharides |
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Definition
| A basic dye that stains acidic molecules...However, acidic ______________ are stained red. |
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Term
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Definition
| Spindle Fiber Aparatus is made up of __________ |
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Term
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Definition
| _____________ interrupts the formation of microtubules |
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Term
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Definition
| freezes cell cycle at metaphase as sister chromosomes CANNOT migrate to opposite poles. |
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Term
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Definition
| ____________ is the uptake of extracellular materials by invagination of the plasma membrane to form small membrane bound ____________. |
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Term
| Phagocytosis, Pinocytosis, Receptor mediated endocytosis |
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Definition
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Term
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Definition
| the process where large particles become internalized in eukaryotic cells |
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Term
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Definition
| uptake of droplets of extracellular fluid into endocytotic vesicles. |
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Term
| receptor mediated endocytosis |
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Definition
| the specific uptake of receptor bound ligands by the invagination of clathrin coated pits on the plasma membrane of animal cells. |
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Term
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Definition
| ___________ will internalize india ink particles using a subtype of __________ |
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Term
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Definition
| The release of intracellular molecules contained within a membrane bound vesicle by fusion of the vesicle with the plasma membrane |
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Term
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Definition
| __________ is important in secretion |
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Term
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Definition
| Tetrahymena have mucous filled secretory vesicles called _______ |
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Term
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Definition
| __________ ___________ is a polynionic dye that triggers ____________ of mucous where the mucous absorbs water and swells into a gel. |
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Term
| Alcian blue, mucopolysaccharides |
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Definition
| ____________ _____________ stains sulfated ___________________, therefore staining the capsule blue |
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Term
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Definition
| The ____________ allows you to determine the cell density or titer of a culture |
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Term
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Definition
| A protein assay that determine protein concentration |
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Term
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Definition
| The Bradford Assay is a assay that follows a color change in the dye _____________ ____________ _______ |
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Term
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Definition
| what binds primarily to arginine residues, but binds minorly to amino acids? |
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Term
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Definition
| in the Bradford assay, measure the ____________ of ___________ to determine protein concentration. |
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Term
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Definition
| The _____________ emits light of a known wavelength through the sample. |
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Term
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Definition
| the light that hits the ______________ (in spectrophotometer) is converted to a numerical value |
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Term
| Abs = Ebc, where E is the extinction coefficient and b is the path length, c is the concentration |
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Definition
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Term
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Definition
| Beer's law states that absorbance is _________________proportional to concentration |
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Term
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Definition
| Limitation of a Bradford assay is that it is linear over a _____________ range |
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Term
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Definition
| ___________ dilutions are often required to dilute proteins to a concentration that is suitable for the Bradford Assay |
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