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Cell Bio Test 4
Cell Bio Test 4
48
Biology
Undergraduate 2
12/15/2010

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Cards

Term

[image]     What are the four macromolecules found in cells?

Definition

[image]      Carbohydrates

[image]      Lipids and Fats

[image]      Proteins

[image]      Nucleic Acids

Term

[image]     In general, how are macromolecules synthesized?

Definition

[image]      Polymerization

[image]      Condensation reaction: a molecule of water is released, an addition of a subunit to an end and a covalent bond is formed

[image]      Condensation reactions are catalyzed by specific enzymes

Term

[image]     What determines the shape of a particular macromolecule?

Definition

[image]      Noncovalent bonds specify the shape of the macromolecule

[image]      Single covalent bonds between subunits allow for rotation of atoms

[image]      Non-covalent bonds that form between different parts of the polymer constrain rotation

Term

[image]     What is the general structure of each type of macromolecule?

 

 

 

 

Definition

[image]      Subunits = monomers (sugars, amino acids, and nucleotides)

[image]      Subunits join together through covalent bonds to create macromolecules

[image]      Macromolecules can join together through non-covalent bonds to form a macromolecule complex

                               

Term

[image]     What are the chemical properties of each of the macromolecules?

Monosaccharides

Definition

[image]      Monosaccharides – (CH2O)n where n = 3, 4, 5, or 6, 2 or more hydroxyl groups, and an aldehyde or ketone

Term

[image]     What is the primary function of each type of macromolecule?

Definition

[image]      Carbohydrates – energy source, energy storage for cells, mechanical support, cell surface markers

[image]      Lipids and Fats – food reserve in cells, form cell membrane to enclose cell organelles, aid in cell signaling, turn on expression of specific types of genes in male development or female development

[image]      Nucleotides – chemical energy, coenzymes (carrier group to take to a specific region of the cell, can enhance reaction rates), signaling molecules, building blocks of nucleic acids (DNA and RNA)

[image]      Proteins – many functions!, building and repair of body tissues, nutrient transport, muscle contraction, source of energy, maintains overall good health

Term

[image]     Identify and explain the levels of protein structure

Definition

[image]      Primary Structure – amino acid sequence

[image]      Secondary Structure – small region of polypeptide chain starts folding, α helix forms from amino acids that are right next to each other in the polypeptide sequence, β-sheet. α helix and β-sheet are the result of hydrogen bonds formed between NH & COOH groups in the backbone of a polypeptide

[image]      Tertiary Structure – 3-D structure of a polypeptide chain. Includes alpha helices, beta sheets, loops, random coils, and random folds.

[image]      Quartenary Structure – complete structure of a protein that has more than one polypeptide chain, polypeptide chains bonded through noncovalent bonds

Term

[image]     What is specificity and how does it relate to proteins and protein binding?

Definition

[image]      The correct ligand binds to the specific protein

[image]      Specificity is determined by protein shape and side chains

[image]      Binding site: the correct must bind to the binding site of the protein to activate the protein

[image]      Regulatory site: other binding sites that cause a conformational change when ligand binds to it and causes the protein to activate or deactivate

[image]      If the wrong ligand binds to the wrong protein it aggregates the cell and can cause damage to the cell

[image]      The correct binding of a ligand to a protein initiates different functions the proteins need to carry out

[image]      Some proteins sole purpose is to bind to a ligand (ex. actin and antibody’s)

Term

[image]     How are proteins controlled?

Definition

[image]      Most proteins and enzymes do not work continuously in the cell so they must be controlled somehow

[image]      Ways proteins are controlled…

[image]      Switching protein activity on or off – enzyme activity changes in response to other specific molecules it encounters, causes change in protein conformation (fast)

[image]      Localizing proteins into specific compartments

[image]      Gene expression – DNA regulates how much transcript is made or destroyed (slow)

Term

[image]     What is allosteric binding, phosphorylation, GTP binding, & ATP binding? How can each be used to regulate a protein?

Definition

[image]      Allosteric binding – an allosteric molecule binds at a site other than the active site which induces a conformational change which can induce a change in enzyme activity. Can have positive regulation if it turns on the enzyme or negative regulation if it turns off the enzyme.

[image]      Phosphorylation – the addition of a phosphate group (negative) to serine, threonine, or tyrosine. This causes a conformational change which either turns the protein on or off.

[image]      GTP binding – GTP proteins can hydrolyze GTP into GDP and cause a slight conformational change turning the protein off. When GTP binds to protein it is turned on again

[image]      ATP binding – hydrolysis of ATP bound to a motor protein such as myosin or kinesin induces a slight conformational change of the motor protein

Term

[image]     Give a general overview of the process of DNA synthesis

Definition
Term
When and why does replication occur?
Definition
Occurs at cell division to double the genetic information
Term

[image]      What is the template used?

[image]    

Definition
  DNA, each strand acts as a template for the synthesis of a new complementary strand (semiconservative)
Term

[image]      What is the product produced?

Definition
2 identical double stranded daughter molecules
Term

[image]     Why does DNA replication have to be so accurate?

Definition

[image]      DNA replication has to be very accurate because if incorrect base pairs are formed and allowed to remain, they would kill the cell through an accumulation of mutations

Term

[image]     What is meant by saying that transcription is a process where there is successive amplification of genetic material?

Definition

[image]      From one piece of DNA, many copies of RNA can be produced

Term

[image]     Give a general overview of the process of transcription

Definition
Term

[image]     What is the general process of transcription?

Definition
RNA polymerase binds to DNA and open up a small region of DNA. RNA polymerase catalyzes the formation of phosphodiester bonds between the ribonucleotides. The ribnucelotides form complementary base pairs with one template strand of DNA. RNA polymerase joins the base pairs by attachment at the growing 3’ end. The RNA strand is displaced from the template and the DNA double helix forms. This allows another RNA polymerase to bind and begin synthesizing another RNA strand which sometimes happens even before the first strand has been completely synthesized.
Term

[image]     Where does transcription occur?

 

Definition

[image]      Cytosol in prokaryotes

[image]      Nucleus of eukaryotes

Term

[image]     What is the template used?

Definition

[image]      The noncoding strand

Term

[image]     What is the product produced?

Definition

[image]      RNA

Term

[image]     What is a coding strand?

Definition

[image]      The DNA strand which has the same base sequence as the RNA transcript produced. This strand contains the codons. The nontemplate strand for transcription which has a sequence equivalent to the transcript.

Term

[image]     What is the non-coding strand?

Definition

[image]      The strand of DNA that does not contain the genetic information necessary to make proteins. It is the template for transcription and it has a complementary sequence to the transcript (RNA) produced

Term

[image]     What is a promoter?

Definition

[image]      A promoter is a specific sequence of nucleotides that indicates the start of transcription

[image]      RNA polymerase binds tightly to DNA at the promoter due to the recognition by its subunit, the sigma factor

[image]      Both prokaryotes and eukaryotes require a promoter

Term

[image]     What is a terminator?

Definition

[image]      A terminator is a specific sequence of nucleotides that indicates the stop of transcription

[image]      RNA polymerase halts and releases the DNA template and newly synthesized stand of RNA at the terminator

[image]      Both prokaryotes and eukaryotes require a terminator

Term

[image]     Give an overview of the process of translation.

Definition
Term

 

[image]     What is the process of translation?

Definition

[image]      Decode mRNA to produce a protein

 

Term

[image]     Where does translation occur?

 

Definition
  [image]   In the ribosomes, on the cytoplasm
Term

[image]     What is the template used?

Definition

[image]      RNA

Term

[image]     What is the product produced?

Definition

[image]      A protein

Term

[image]     What is meant by the term genetic code?

Definition

[image]      The set of rules specifying the correspondence between nucleotide codons in DNA or RNA and amino acids in proteins

Term

[image]     What are codons, anticodons, tRNA (aminoacyl tRNA, peptidyl tRNA), and ribosomes?

Definition

[image]      Codons – sequence of 3 nucleotides in a DNA or mRNA molecule that represent the instructions for the incorporation of a specific amino acid into a growing polypeptide chain. The start codon AUG codes for an amino acid, methionine. The 3 stop codons, UAA, UAG, UGA, are not recognized by tRNA and do not specify an amino acid, but they instead signal to the ribosome to stop translation

[image]      Anticodons- sequence of 3 nucleotides in a tRNA molecule that is complementary to the 3 nucleotide codon on a mRNA molecule; each anticodon is matched to a specific amino acid covalently attached elsewhere on the tRNA molecule

[image]      tRNA – a set of small RNA molecules used in protein synthesis as an interface between mRNA and amino acids (binding partner = specific amino acid)

[image]      aminoacyl tRNA  site where aminoacyl tRNA enters ribsome and binds to codon in mRNA

[image]      peptidyl tRNA – site where growing polypeptide chain is attached to tRNA

[image]      ribosomes – particle composed of ribosomal RNA’s and ribosomal proteins that associates with mRNA and catalyzes the synthesis of a protein. Has 2 subunits, the large subunit which catalyzes the formation of peptide bonds and the small subunit which matches tRNA codons to mRNA.

Term

[image]     Which amino acid is translated first?

 

 

Definition

[image]      Methionine, the start codon

Term

[image]     Is a newly synthesized polypeptide always a functional protein? Explain.

Definition

 

[image]      No, sometimes proteins require further attention before they are useful to the cell. For example, covalent modification (like phosphorylation), the binding of small-molecule cofactors, or the association with other protein subunits may be needed to activate a newly synthesized protein.

Term

[image]     Compare and contrast replication, transcription, and translation in eukaryotes and prokaryotes.

[image]      Eukaryotes

Definition

[image]      DNA à Transcription à5’ Capping, RNA splicing, 3’ Polyadenylation à  mRNA àExport of mRNA to cytoplasm à Translation (initiation, elongation, termination) à Protein!

Term

Compare and contrast replication, transcription, and translation in eukaryotes and prokaryotes.

- Prokaryotes

Definition

[image]      DNA à Transcription à mRNA àTranslation (initiation, elongation, termination) à Protein!

Term

[image]     If given a sequence of DNA, you should be able to determine the unprocessed mRNA sequence, the mature (processed) mRNA sequence, and the amino acid sequence of the protein produced from this sequence. 

Definition
Term

[image]     What is gene expression?

Definition

[image]      The process by which information from a gene is used to produce a functional gene product (protein or RNA) with a characteristic activity

Term

[image]     At what stage can gene expression be controlled?

Definition

[image]      Transcriptional control – change rate at which transcription initiates

[image]      RNA processing – addition of poly-A tail and 5’ cap

[image]      Splicing – splice different ways to make different transcripts which makes different proteins

[image]      Degradation – change rate

[image]      Translation – control initiation

[image]      Protein Activity – presence of kinase or phosphotase

Term

[image]     Which stage is most commonly regulated to control gene expressions?

[image]       

 

Definition

[image]      Transcriptional control – doesn’t waste energy making RNA or protein

Term

[image]     What are DNA regulatory sites and how can they be used to regulate transcription?

Definition

[image]      Transcription regulators bind to the regulatory DNA sequence which acts as a switch to control transcription. The regulatory sites influence the rate of transcription and determine what regulatory protein binds

[image]    

Term
Explain how a single transcription regulatory protein can affect the expression of many genes.
Definition

[image]      You can add an additional transcription regulator that can bind to the regulatory sequences of each gene which combine for efficient initiation of transcription, and the genes will be switched on as a set.

[image]      Cells may want to do this to create different cell types

Term

[image]     What are some post-transcriptional controls used to contribute to gene expression?

[image]   

Definition

   Alternative Splicing – allows different forms of a protein to be made in different tissues

[image]      Riboswitches – short sequences of RNA that change their conformation when bound to small molecules. Each riboswitch recognize a specific small molecule. The conformational change that is driven by the binding of that molecule can regulate gene expression.

[image]      Untranslated regions of mRNA control initiation of translation – repressors inhibit translation initiation by binding to specific RNA sequences in the 5’ untranslated region of the mRNA and keeping the ribosome from finding the first AUG.

[image]      miRNA’s black translation of genes – controls gene expression by base-pairing with specific mRNAs and controlling their stability and translation. Forms complex with RISC proteins.

Term

[image]      Fatty Acids 

Definition
Fatty Acids carboxyl group with long hydrocarbon tail, amphipathic, differ from each other by number of carbons in tail, and differ by number and position of double bonds they contain in their tail. All single bonds in hydrocarbon tail = saturated, one or more double bonds in hydrocarbon tail = unsaturated
Term

[image]      Amino acids

Definition
Amino acids central carbon = α carbon, amino group (positive), carboxyl group (negative), side chain group gives the amino acid its identity
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