Term
resolution
eye, light microscope, e- microscope |
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Definition
closest you can get 2 objects and still see them as seperate
.2mm, .2 micro m, .2 nm |
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Term
| bright field microscopes: 2 types |
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Definition
| simple (like magnifying glass), compound (series of lenses) |
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Term
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Definition
| best for pale, living objects- only can see specimen, oblique light rays |
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Term
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Definition
| examine living organisms, see detail inside |
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Term
| Nomarski (Phase differential Interference) |
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Definition
| topological details, living organisms |
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Term
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Definition
| absorb UV light then flouresce back |
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Term
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Definition
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Term
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Definition
| vacuum -> must be completely dehydrated. detailed view, 2 types: TEM (beam through specimen), SEM (blow metal on outside of specimen, computer analyzes image from e-s that bounce off. ) |
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Term
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Definition
| cation bcc bacteria are always negative |
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Term
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Definition
| crystal violet etc. only one stain. |
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Term
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Definition
| Gram, Acid-fast, Endospore |
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Term
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Definition
| Negative Capsule, Flagellar, Flourescent |
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Term
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Definition
flood w/ crystal violet: all cells purple.
flood w/ iodine: iodine= mordant, all cells purple
flood w/ alcohol and acid: gram - lose color
flood w/ safranin: gram - turn pink |
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Term
| Identifying Characteristics: 5 |
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Definition
| Phage typing, Nucleic acids, Physical, Biochemical, Serological (Parents Neglect PBS) |
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Term
| Which stains use a mordant? |
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Definition
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