Term
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Definition
template DNA
oligonucleotide primers
Taq DNA polymerase
dNTPs
buffer
MgCl2 |
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Term
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Definition
| co-factor for taq polymerase |
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Term
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Definition
1. denaturing (95)
2. annealing (55)
3. extension/elongation (72) |
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Term
| polyacrylamide gels are used for.... |
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Definition
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Term
| agarose gels are used for |
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Definition
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Term
| how are gels seen after they are run? |
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Definition
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Term
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Definition
| naturally occur in prokaryotes
recognize foreign DNA sequences and cut it into pieces |
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Term
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Definition
| determine target specificity |
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Term
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Definition
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Term
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Definition
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Term
| What pair forms 3 Hydrogen bonds? |
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Definition
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Term
| What pair forms 2 Hydrogen bonds? |
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Definition
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Term
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Definition
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Term
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Definition
| 1 strand is separated from the other
20 seconds
disrupts H bonds |
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Term
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Definition
| annealing of primers to single stranded DNA template
45 seconds |
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Term
Agarose Gel Electrophoresis
1) what is molecular size marker?
2) segments separated by...? |
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Definition
1)HindIII digested lambda DNA
2)separates DNA fragments by size |
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Term
| defense against bacteriophages |
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Definition
| restriction endonucleases |
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Term
SS 16S
1)used for?
2) what do they contain thatr is useful for bacterial identification? |
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Definition
1) phylogenic studies
2) hyper variable regions which can provide species-specific signature sequences for bacterial identification |
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