Term
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Definition
Granulocytes
Neutrophils
- Most numerous
- 3-5 lobed nuclei
Basophils
- Least numerous
- Histamine and heparin
- (2) Viral infections
- Nucleus obscured by deeply stained basophilic granules (stained by Methylene blue)
Eosinophils
- Major basic protein-->cytoplasm filled with red granlues stained by eosin
- Bilobed nuclei
- (1) Allergic reaction, and (2) parasite
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Term
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Definition
Eosin
- Acidic dye/neagatively charged
- Stains acidophilic/basic/positively charged structures
- Redish pink
Methylene Blue
- Basic dye
- Stains basophilic structures/acidic sturctures like:
- DNA, RNA
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Term
| Hemorrhage indicated by what in PBS |
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Definition
Normocytic nucleated RBCs
Macrocytic if extreme |
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Term
| Reticulocyte count seen using what dye |
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Definition
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Term
| Supra-vital-stain indicates |
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Definition
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Term
| Corrected reticulocyte count |
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Definition
(Actual Ht/45) x reticulocyte count
Divide by 2 if polychromasia present (due to degree of anemia)
Ht= Hbx3 |
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Term
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Definition
| Size, shape, color, and inclusions of RBCs |
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Term
|
Definition
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Term
|
Definition
Microspherocyte- hereditary spherocytosis, hemolytic anemia
Sickle cell- homozygous sickle cell
Tear-drop cell- bone marrow infiltration/myelofibrosis
Schistocyte- [microanglopathic] haemolytic anemia |
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Term
| Abnormal inclusions of RBCs |
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Definition
Howell-Jolly bodies- asplenia
Basophilic stippling- Pb poisoning
- Ferrochelatase inhibition
- Spleen not working correctly
Cabot's ring bodies- MTs
Heinz bodies- denatured Hb- G6P deficiency |
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Term
|
Definition
Aspirate- take fluid
Biopsy- take bone with it
-ID hypocellular, normal, hypercellular (cancer) |
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Term
|
Definition
-Infections (appendicitis)
-MI
-Corticosteroids- decrease adhesion, increased release from BM
Shift to left- more band cells
Sepsis or severe inflammation may cause morphological changes:
-toxic granules- abnormal primary granules, darker than normal
-Dohle bodies- dull gray inclusion; patches of dilated RER
-Cytoplasmic vacuoles- phagolysosomes indicating phagocytosis |
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