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Biotechnology
Biotechnology
92
Biology
11th Grade
11/16/2008

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Term
Biotechnology
Definition
Any technique that uses living organisms, or parts of organisms, to make or modify products, improve plants or animals, or to develop microorganisms for specific uses.
Term
Early forms
Definition
1. cheese making
2. wine making
Term
modern forms
Definition
1. genetic engineering
2. clonning
3.cell culturing
Term
yogurt
Definition
yogurt is a food produced by the fermentation of milk by bacteria
Term
bacteria involved in yogurt fermintation
Definition
1. streptococcus thermophilus
2. lactobacillus bulgaricus
Term
why is yogurt sour
Definition
lactic acid being formed by the bacteria
Term
what gives yogurt the semi solid apperance
Definition
increase in lactic acid causes the casein micelles to unravel and form a mesh like structure
Term
what are the four components of milk
Definition
1. water
2. fat
3. casein micelles
4. lactose
Term
what does streptococcus and thermophilus mean
Definition
- a chain of spherical bacteria
- bacteria can live in high tempritures
Term
what does lactobacillus and bulgaricus mean
Definition
-a rod shaped bacteria that can convert lactose in milk to lactic acid
- explains where the bacteria was first seen (bulgaria)
Term
what does acetaldehyde do to yogurt
Definition
it gives it its pungent smell and taste.
Term
what is the word and chemical equasion for fermintation of lactose
Definition
lactose - lactic acid + 2ATP

C6 H12 O6 - 2C3 H6 O3
Term
What are bacteria
Definition
simple cells
Term
what 4 names refer to the shape of bacteria
Definition
1. cocci
2. bacilli
3. spirilli
4. Vibrios
Term
what names sugest cluster and chains of bacteria
Definition
- staphylo
- strepto
Term
what happens to bacteria when conditions are favourable
Definition
reproduce by binary fusion to produce genetically identical daughter cells
Term
what happens to bacteria when conditions are not favorable
Definition
bacteria forms spores
Term
what does phytosynthetic mean
Definition
capeable of making their own food
Term
what does heterophilic mean
Definition
not capable of making their own food
Term
how do heterotrophic bacteria eat
Definition
secrete enzimes onto a food source, digest the food outside the cell and then obsorb the souble food into the cell by diffusion
Term
what are bacteria called when they do and do not need oxygen
Definition
aerobic
anaerobic
Term
what is the range of ph levels in which acidophillic, neutrophilic and alkaliphilic bacteria live in
Definition
2.5-5.5
5- 9
8- 11
Term
what are enzimes
Definition
enzimes are biological catalysts that are made inside cells but can function internally and externally
Term
are enzime activities effected by tempritures
Definition
yes. when the temp is above 50 deg c when the shape of the active site changes perminently
Term
what is enzime activity effected by
Definition
chemical inhibitors which compete with the subtrate molecule for the enzimes active site.
inhibitrs either temporairily or perminitly block the active site.
Term
what is DNA
Definition
an important molecule of hereditry in all living things.
Term
what does DNA look like
Definition
DNA is a double sided molecule made of two chains of neucleotides
Term
what else can DNA be described as
Definition
lader- like molecule
where the sides are sugar- phosphate
backbones and runs are complementery pairs
Term
what are the four bases that pair up
Definition
Thymine- adenine
guanine- cytosine
Term
what does DNA carry
Definition
it carries hireditory information in the form of genes
Term
what is a gene
Definition
a gene is a small section of the DNA molecule which contains a coded message
Term
how is the coded message stored
Definition
as a sequance of bases on one side of the DNA molecule
Term
what is a chromosone made up of
Definition
DNA and contains many genes
Term
what is one side of the DNA a coded message for
Definition
polypeptide (protein)
Term
what is a common organism used in genetic engineering
Definition
bacteria
Term
what are plasmids used for
Definition
transfer a selected gene into a bacterium
Term
what enzime is used to cut the plasmid
Definition
restriction enzime
Term
what enzime is used to glue the plasmid back together
Definition
ligase enzime
Term
what is done to the gene for making human insulin
Definition
found and isolated from the human genome
Term
how is the gene transfered to a suitable bacterium
Definition
using restriction and ligase enzimes the gene can be spliced into a plasmid and transfered
Term
why is bacteria a perfect host cell for the human gene
Definition
a clone of the identical cells can be producing during binary fussion
Term
why is agrobacterium tumefaciens used by genetic engineeres to transfer selected genes into plant cells
Definition
its a natural enemy and can be used as a vehicle to transfer genes into food producing plants
Term
how is the gene transfered to the plant
Definition
spliced into a plasmid and transfered to the agrobacterium tumefaciens
Term
what was used in early GE
Definition
antibiotic reistance genes called marker genes, where also inserted into the plasmid. some scientists beleive that this is what accelerated the evolution of antibiotic resistance in bacteria
Term
how are the agrobacterium tumefaciens transfered into the plant
Definition
small peices of plant chosen to be GE are placed in a culture medium and covered with the GE agrobacterium tumefaciens
Term
what causes the plant tissue to begin to grow roots and a stem
Definition
further culturing with growth factors
Term
whay are the plants then grown in soil in a glasshouse
Definition
to test for characteristics of the gene and to test for problems the GE plant may have on humans, the enviroment and other species.
Term
what is GE on human cells called
Definition
gene theropy
Term
how are diseases such as cystic fibrosis being treated
Definition
by using GE viruses
Term
what is the problem with cistic fibrosis patients
Definition
too much salt inside their cells which results in an osmotic inbalance and water entering the cells
Term
what is the build up of salt due to
Definition
a faulty cistic fibrosis transfer gene (CFTR)
Term
what is the vector for transfering the healthy cistic fibrosis transfer gene into the lung cells of a CF patient
Definition
adeno- virus
Term
what does the CFTR gene do once inside the cell
Definition
makes a protein that allows the cell membrane to allow salt to leave the cells to restore the correct water levels inside the lung cell
Term
what is an alternative method to transfer the genes inside the lung cell
Definition
liposomes
Term
what are liposomes
Definition
fat droplets into which the genes can be hidden. lung cells will accept the liposomes as food and constantly take in the hidden genes
Term
what are the 3 benefits of GE
Definition
1. cheaper medicine production
2. more nutritionous foods
3. crops better suited to saline soils and drought conditions
Term
what are the concerns for genetic engineering
Definition
1. safety
2. jumping genes may cause an evolution of super weeds
3. acceleration of antibiotic resistant bacteria
Term
what are 3 ethnical concerns
Definition
1. companies like monsanto have a conflict of interest with making pesticides and herbisides as well as GE crops
2. genes can be patented
3. threat to third world countries
Term
what is ecology
Definition
the study of living organisms in relation to their enviroment
Term
what is an ecosystem
Definition
the biotic and abiotic features of an enviroment
Term
what is a community
Definition
all the populations of animals and plants (biotic factors) occupying a location
Term
what are abiotic factors
Definition
non living factors in an ecosystem
Term
what are biotic factors
Definition
living factors in an ecosystem
Term
what are autotrophs
Definition
capable of making their own food (producers)
Term
what are heterotrophs
Definition
not capable of making their own food (consumers)
Term
whatr is marine ecology
Definition
the study of the relationships between organisms that live in the oceans of the world
Term
what are plankton known as
Definition
the drifters of the sea
Term
what are phytoplanktons
Definition
producers
Term
what is the most numerous of phytoplanktons
Definition
diatoms
Term
what are zooplanktons
Definition
consumers
Term
what is the remains of dead organisms in the ocean called
Definition
detritus
Term
what are food chains used for
Definition
to show the feeding relationships and flow of energy in an ecosystem
Term
what is at the start of a food chain
Definition
producer
Term
what does a biomass pyramis show
Definition
the dynamics of energy in an echosystem
Term
what does a food web show
Definition
all the feeding relationships and flow of energy in an echosystem
Term
what do addaption of animals enable them to so
Definition
survive in their enviroment
Term
what do adaptions help an organism to fufill
Definition
1. food/ water
2. protection
3. reproduction
Term
what are structual adaptions related to
Definition
physical features of an organism
Term
what are behavioural adaptions related to
Definition
actions of an organism
Term
what are phsycological adaptions related to
Definition
the internal functioning of an organism
Term
what is the structial adaptation of fins on fish used for
Definition
the controlled movement in the ocean
Term
name 6 fins
Definition
1. spiny dorsal fin
2. soft dorsal fin
3. caudal fin (tail)
4.anal fin
5. pelvic fin
6. pectoral fin
Term
all organisms need to exchange gass with their enviroment by what
Definition
difusion
Term
what are the 3 characteristics of gass echange surface to increase the rate of difusion
Definition
1. large surface area
2. thin
3. moist
Term
what needs to difuse in and out of fishs blood
Definition
oxygen
carbon dioxide
Term
what do gills of fish do
Definition
extract oxygen from the water
Term
how is the rate of diffusion increased
Definition
counter- current system where the water and blood flow in oppisate directions
Term
what are the 5 characteristics of sewage
Definition
99% water
disolved organic matter
high nutrient levels
ten high levels of heavy metals
chemical toxins
Term
how do toxic algal blooms begin
Definition
discharge of sewage with high neutrient levels which accelerate the growth of blue green algae
Term
what does an algal bloom result in
Definition
depletion of oxygen
death of organisms due to the build up of toxins in the water
Term
what is the depletion of oxygen in water called
Definition
eutrophication
Term
what does sewage with high organic matter do
Definition
provide food for aerobic bacteria as they consume the organic matter they demand oxygen
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