Term
|
Definition
| cut double stranded DNA at specific sequences. Used in cloning and some diagnostic assays |
|
|
Term
|
Definition
| makes a new DNA molecule that is complementary to an existing template strand. |
|
|
Term
| Heat Stable DNA polymerases |
|
Definition
| isolated from thermophilic bacteria, that remain active at high tempteratures. Example - Taq. Uses include PCR and cycle sequencing. |
|
|
Term
|
Definition
| forms a phosphodiester linkage between 5'-phosphate and the 3'-hydroxyl of adjacent nucleosides on nicked DNA. Used in cloning. |
|
|
Term
|
Definition
| makes an RNA molecule that is complementary to an existing DNA template. |
|
|
Term
|
Definition
| makes a DNA molecule that is complementary to an RNA template. Use in the production of cDNA and to identify transcriptional start-sites of genes. |
|
|
Term
|
Definition
| a sample of dsDNA is heated to separate into ssDNA, then a probe(which is an oligonucleotide) is added which binds to complementary sequence on DNA and has a label that can later be activated to indicate the presence of the sequence of interest |
|
|
Term
| Uses of DNA Hybridization |
|
Definition
| Northern Blot (detecting RNA), Southern Blot (detecting DNA), genetic disease diagnosis, pathogen identification, microarrays (assaying gene expression) |
|
|
Term
|
Definition
| artificially produced sequences of DNA that are formed from fragments of nucleic acid not normally found in nature |
|
|
Term
|
Definition
| recombining DNA from one source into another location so that it can be maintained |
|
|
Term
| Polymerase Chain Reaction (PCR) |
|
Definition
| amplifies sequences from a small sample of DNA |
|
|
