Term
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Definition
| A biological agent with the potential to produce harmful effects in humans |
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Term
| what are biological agents? |
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Definition
| Viruses, bacteria, fungi (molds and yeasts) |
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Term
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Definition
| Ability of an organism to cause disease in humans |
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Term
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Definition
| Laboratory aquired infections |
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Term
| What are laboratory acquired infections? |
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Definition
| Infections caused by laboratory organisms |
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Term
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Definition
| very small particles or droplets that contain bio agents in the air |
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Term
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Definition
mixing and pouring liquids,
pipetting lilquids,
centrifuging cultures,
inserting a hot loop into a bacterial culture |
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Term
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Definition
| Center for disease control |
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Term
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Definition
health and human cervices
prevention and control of disease, injury and disability |
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Term
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Definition
| National institues of health |
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Term
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Definition
Federal funding for biomedical research
provices guidelines for lab safety. |
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Term
| What are some biohazardous microorganisms? |
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Definition
| Bacteria, fungi protozoan parasites and viruses |
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Term
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Definition
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Term
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Definition
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Term
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Definition
small
contains rna or dna surrounded by protein coat
easy to aerosol
HPV, HIV, HBV, |
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Term
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Definition
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Term
| What are overal biosafety themes? |
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Definition
Personal protective equipment or PPE
Containment conrol of biohazards by isolation |
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Term
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Definition
| control of biohazards by isolation and separating from humans. |
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Term
| What are universal precautions? |
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Definition
| standard safety practices for handling human blood products |
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Term
| what is aseptic technique? |
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Definition
| practice that minimize risk of contamination |
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Term
| What are bio-safety levels? |
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Definition
| level of the biocontainment precautions required to isolate dangerous biological agents in an enclosed facility |
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Term
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Definition
| Well classified organisms that DO NOT cause disease |
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Term
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Definition
| organisms that may cause disease that is MILD OR TREATABLE |
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Term
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Definition
| Serious or lethal disease causing organisms |
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Term
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Definition
| High risk of life threatening disease!!!!! |
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Term
| What is the difference between BSL and BSC? |
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Definition
| BSL is biosafety level and BSC is biological safety cabinet |
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Term
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Definition
Containment for aerosols
separate material from operator and lab
enclosed CLEAN air envioronment |
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Term
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Definition
High efficienty particualate air,
higly pleated glass and paper fibers |
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Term
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Definition
| REMOVE PARTICLES .3uM or larger |
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Term
| What is a laminar flow cabinet? |
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Definition
| lab hood with sterile directed air flow |
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Term
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Definition
Horizonal laminar flow HOOD
provides sterile work surface but NOT worker protection
NO WORKER PROTECTION!!!!! |
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Term
| What best describes a laminar flow hood? |
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Definition
| Sterile work surface, but NO WORKER PROTECTION!!!! |
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Term
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Definition
Reduces aerosol escape
DOES NOT MAINTAIN WORK SURFACE STERILITY!!!!! |
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Term
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Definition
Reduces aerosol excape
MAINTAINS surface sterility |
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Term
| What is the most common bsc??? |
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Definition
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Term
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Definition
Glove box
TOTAL CONTAINMENT
EXTREMELY BIOHARARDOUS AGENTS |
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Term
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Definition
| chemicals that kill microorganims!!! |
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Term
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Definition
| removal of all pathogens from surface |
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Term
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Definition
| general reduction fo pathogens |
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Term
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Definition
| killing ALL organisms on a surface=disinfection |
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Term
| What is central tendencyh? |
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Definition
| description of center of data set |
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Term
| what is measures of dispersion? |
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Definition
| how much vales in a data set vary |
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Term
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Definition
| difference between highest and lowest value |
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Term
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Definition
| distance of a data point from the mean |
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Term
| what are two ways to meaure deviation/ |
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Definition
| variance and standard deviation |
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Term
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Definition
| sum of all values divided by number=AVERAGE |
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Term
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Definition
| middle value of data halfway between highest and lowest value |
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Term
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Definition
| value that occurs most frequently!!!! |
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Term
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Definition
sum of deviations divided by n-1
=E (x-`x)^2 divided by (n-1) |
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Term
| what is standard deviation? |
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Definition
| square root of (the sum from mean divided by n-1) |
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Term
| what reduces variability? |
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Definition
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Term
| what reduces variability? |
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Definition
examine proces for malfunctions or problems
determine certainty of a measurement result in the presence of variability |
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Term
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Definition
| a measurement that has much higher or much lower value then rest of data |
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Term
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Definition
| represents unit of measure |
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Term
| what are primary standards |
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Definition
| accepted without verification |
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Term
| what are secondary standards |
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Definition
| based up measurements from primary st |
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Term
| what are worknig standards? |
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Definition
| standards used in individual labs based on sec stand |
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Term
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Definition
| international organization for standardization |
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Term
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Definition
| American national standards institute |
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Term
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Definition
| National institute for standads and technology |
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Term
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Definition
| american society for testinga nd materials |
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Term
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Definition
| adjustment of meas system to be in accordance with standard |
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Term
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Definition
| error that can be tolerated in calibration |
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Term
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Definition
| a check on performance of instrument |
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Term
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Definition
| chane of calibrations to establish value of standard |
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Term
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Definition
| diffence between measured value and true value |
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Term
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Definition
| huge errors caused by blunders!!! |
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Term
| what is systematic error? |
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Definition
| bias measurements that tend to be higher or lower than true value. |
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Term
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Definition
| using fthings for measurements that vary from others |
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Term
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Definition
environmental condition that alter results
rosponst of intruments drift over time. |
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Term
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Definition
| variation by chance but overall average to correct or expected value. |
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Term
| what is absolute error calc? |
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Definition
| true value minus average measured value |
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Term
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Definition
| true minus average divided by true times 100% |
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Term
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Definition
| systematic error. measure how close data is to true value |
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Term
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Definition
random errors.
consistency of series of tests |
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Term
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Definition
measurements made under uniform conditions and their precision
measure 100ul 1000 times. |
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Term
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Definition
| precision made under two different laboratories |
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Term
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Definition
| combination of all random and systemic error that effect results |
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Term
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Definition
| effects of ONLY RANDOM ERROR |
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Term
| HOW TO DETERMINE PRECISION? |
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Definition
| range sd and cv which is sd100 dividing by mean |
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Term
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Definition
| no more numbers right of decimal than least number of places. |
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Term
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Definition
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Term
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Definition
| mechanical and electronic |
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Term
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Definition
beams and wights
object placed on pan attached to beam and balanced on standard |
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Term
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Definition
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Term
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Definition
air currents
temperature
static charge |
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Term
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Definition
| least to highest wight that can be measure |
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Term
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Definition
| how much highest that can be weighed |
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Term
| sensitivity or radability? |
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Definition
| smallest unit taht can be measured. |
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Term
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Definition
| broad range accuracy how far can it measure up and down. |
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Term
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Definition
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Term
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Definition
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Term
| what is serological pipette? |
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Definition
| must be blown out to deliver full volume |
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Term
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Definition
| tip is not part of measurement |
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Term
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Definition
| measuring assistant devices |
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Term
| what is volumetric pipette? |
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Definition
| measures only one volume usually |
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Term
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Definition
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Term
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Definition
| air cushion no touch pip to sample |
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Term
| what is reverse mode pipetting? |
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Definition
depress to second stop to take up sample
release plunger
depress plunger to first stop
tip is discarded
measure of volatile liquids and viscous samples. |
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Term
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Definition
| fine liquid droplets that remain supended in the AIR |
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Term
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Definition
| contamination from sample 1 to another |
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Term
| how to minimize contamination? |
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Definition
tips with filter
dedication of pipet
proper operaton |
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Term
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Definition
| light passes through without interaction |
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Term
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Definition
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Term
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Definition
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Term
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Definition
| light energy taken up by material |
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Term
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Definition
| measures light efect on sample |
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Term
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Definition
| distance from crest to crst of each wave. |
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Term
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Definition
| light through sample divided by light through blank |
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Term
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Definition
| transmittance smaple over blank times 100 |
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Term
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Definition
optical density
-logt or 2-log percent T |
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Term
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Definition
| absorbance of light is directly prioportional to concentation |
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Term
| what is chromatography used for? |
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Definition
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Term
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Definition
| size exclusion, ion, hydrophics |
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Term
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Definition
| bacterial lysate to matricx in high salt buffer |
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Term
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Definition
| less hydrophobs leave column in medium salt buffer |
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Term
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Definition
| gfp is eluted from column with low salt buffer |
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