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biology test 4
chapter 14
20
Biology
Undergraduate 1
04/12/2012

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Term
In 1952, Hershey and chase has conclusively proved what?
Definition
that DNA is the hereditary molecule.
Term
what was found in phage-infected cells and in progeny phages, showing that DNA is the genetic material?
Definition
32P (used to label DNA)
Term
what was found in phage coats after infection, but was not found in the infected cell or progeny phages, showing that protein is not the genetic material?
Definition
35S (used to label proteins)
Term
What are the four bases?
Definition
Bases A and G
bases T and C
Term
Which bases are purines?
Definition
Bases A and G
Term
Which bases are Pyrimidines?
Definition
bases T and C
Term
What are the differences between a purine and pyrimidine?
Definition
Purine have two carbon-nitrogen rings.
Pyrimidine have single carbon-nitrogen ring
Term
Erwin Chargaff discovered what?
Definition
that nitrogenous bases in DNA occur in definite ratios.
Amount of adenine equals amount of thymine
Amount of guanine equals amount of cytosine
Term
Who came up with the blueprint of what a DNA look like?
Definition
Watson and Crick
Term
What is semiconservative replication?
Definition
Each new double helix has one old strand, derived from the parental DNA molecule, and one new strand
Term
Lagging strand
Definition
The new strand, moving along the template, 5’→ 3’ direction
Term
Leading strand
Definition
old strand, “read” in the 3’ → 5’ direction.
Term
DNA polymerase
Definition
extend the new DNA strand, one nucleotide at a time, by moving along the template
Term
Sliding DNA clamp
Definition
tethers DNA polymerase to the template strand and increases rate of DNA synthesis
Term
DNA helicase
Definition
unwinds the DNA strands, producing a Y-shaped replication fork
Term
Single-strand binding proteins
Definition
keep the strands from apart
Term
Topoisomerase
Definition
prevent over-twisting
Term
Primase
Definition
attach a short RNA primer to the template DNA
Term
DNA ligase
Definition
binds Okazaki fragments together
Term
How does telomerases solve a particular replication problem? What is that problem?
Definition
telomerase will stop the shortening of telomeres. the lost of telomeres
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