Term
| Light energy absorbed by cholorphyll... |
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Definition
a) permits the removal of electrons from water
b) the shuttling of the electrons and protons that drive the generation of ATP and reduce CO2 |
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Term
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Definition
| pattern of absorption by a substance |
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Term
| chlorophyll a is soluble in... |
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Definition
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Term
| chlorophyll b is soluble in... |
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Definition
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Term
| carotene is soluble in... |
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Definition
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Term
| location of petroleum ether in a test tube |
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Definition
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Term
| All pigments absorb the most light at |
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Definition
450 and 650 nm wavelength
blue red |
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Term
| Carotene absorbs only _______ light |
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Definition
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Term
| chlorophyll a & b absorb _______ and ______ light |
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Definition
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Term
| All pigments flatten (don't absorb ________ color) |
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Definition
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Term
| A peak in the graph means... |
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Definition
| that chlorophyll aborbs light at that wavelength |
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Term
| In an absorption vs. wavelength graph... |
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Definition
| aborption is on the y-axis and wavelength is on the x-axis |
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Term
Independent Variable
(in chlorophyll extraction lab) |
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Definition
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Term
Dependent Variable
(in chlorophyll extraction lab) |
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Definition
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Term
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Definition
| When there is no blank, set the left knob to zero transmittance which is 100 absorption. Insert blank tube, set right knob to zero absorption, which is 100 transmittance. CALIBRATED! |
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Term
| What would be the consequences to photosynthesis if the chlorophyll molecule were transparent to light? |
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Definition
| The chlorophyll molecule would absorb a lot of light at all wavelengths. |
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Term
| What would the absorption spectrum of a colorless, transparent substance look like? |
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Definition
| The graph would be a straight line |
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Term
| What major property of chlorophyll a&b and carotenoids did we take advantage of in order to separate them from one another? |
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Definition
| Their solubility in different solutions |
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Term
| Carotene pigment flattens (doesn't absorb) ______ light |
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Definition
orange and red
600nm 650nm |
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Term
| Electron deficit in chlorophyll during the oxidation of water is filled by... |
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Definition
| electrons that are removed from water |
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Term
| Goal of Oxidation of Water Lab |
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Definition
| to measure the rate at which water is oxidized during photosynthesis when we vary the amount of chloroplasts participating in the rxn |
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Term
Independent Variable
(oxidation of water) |
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Definition
| amount of chloroplasts added to the rxn mixture |
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Term
Dependent Variable
(oxidation of water) |
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Definition
rate of water oxidation
*measured as the rate of DPIP reduction |
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Term
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Definition
it looses its blue color
*this makes it easy to measure with the spec 20 |
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Term
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Definition
(Absorption 10 - Absoption 0)/
(Time 10 - Time 0)
Change in Absorption/
Change in Time |
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Term
What is responsible for the oxidation of water?
(reduction of DPIP) |
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Definition
| light! b/c photosynthesis needs light to oxidize water and release electrons |
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Term
| Why was bisulfite added to the blank tubes in the experiment with chloroplast function? |
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Definition
| The bisulfite reduced the DPIP and caused the test tube to be clear again. We don't want the blank to have color, because then it will reflect that light |
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Term
| Function of chloroplast.. |
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Definition
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Term
| What role does oxygen play in photosynthesis? |
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Definition
| Oxygen plays no direct role in photosynthesis, but is vital to cellular respiration |
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Term
| What role does oxygen play in cellular respiration? |
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Definition
| oxygen allows many organisms, plants included, to fully extract the potential energy of glucose |
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Term
| Goal of Production of Oxygen Lab |
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Definition
| To study the effect of light quantity on the rate of oxygen production by an alga called Chlorella. |
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Term
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Definition
| member of the phylum Chlorophyta; an aquatic photosynthetic unicellular organism |
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Term
Independent Variable
(Production of Oxygen Lab) |
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Definition
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Term
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Definition
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Term
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Definition
| way of measuring light quantity |
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Term
Dependent Variable
(Production of Oxygen Lab) |
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Definition
| rate at which Chlorella produces oxygen |
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Term
1mV=
How to calculate the mg/L of dissolved oxygen |
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Definition
.0899 mg/L of dissolved oxygen
1mg/L 92.29
------ x ------
X 8.3mg/L
Computer read out (mV)
-----------------
dissolved oxygen conc. (mg/L) |
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Term
| As PFR increases what happens to oxygen production rate? |
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Definition
it also increases
As there is less light/larger PFR nummber, oxygen production rate increases. |
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Term
| What happened to oxygen production rate in the dark Chlorella tube? |
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Definition
| Oxygen production decreases in the dark |
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Term
| For what do plants use oxygen in cellular respiraton? |
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Definition
| To extract energy from glucose |
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Term
| If the transfer of electrons were blocked at PSII, how would the production of oxygen in plants be affected? |
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Definition
| The plant would no longer be able to oxidize water to produce oxygen |
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Term
| How would the blockage of the transfer of electrons out of PSII affect the production of glucose in a plant? |
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Definition
| The plant wouldn't be able to perform cellular respiration without oxygen, so the Krebs Cycle could never begin, and glucose would never be produced |
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Term
| Why was a buffer used for the isolation and function assessment of chloroplasts? |
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Definition
| Because the buffer kept a constant pH to keep the chloroplast functioning at optimal activity |
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Term
Where do the electrons that reduce DPIP come from?
Where do these electrons end up? |
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Definition
oxidation of water in the light rxns
NADPH --> Calvin Cycle |
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