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Biology Lab 1105
Exercise 2,3,9
52
Biology
Undergraduate 1
09/21/2009

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Term
light microscope
Definition
A coordinated system of lenses arranged to produce an enlarged, focusable image of a specimen using light.
Term
magnifies
Definition
increases its apparent size
Term
resolution
Definition
ability to distinguish two points as separate points
Term
contrast
Definition
amount of difference between the lightest and darkest parts of an image
Term
light source
Definition
light bulb located at the base of the microscope that illuminates the specimen by passing light through a thin, almost transparent part of the specimen
Term
condenser lens
Definition
Located immediately below the specimen, focuses light from the light source onto the specimen.
Term
condenser iris diaphragm
Definition
regulates the amount of light reaching the specimen
Term
objectives
Definition
a series of several lenses that magnify the image mounted on the revolving nosepiece
Term
Four Objectives
Definition
low magnification(4X), medium magnification(10X), high magnification(40X), oil immersion(100X)
Term
body tube
Definition
metal casing through which light passes to the oculars
Term
oculars
Definition
lens you look through(10X)
Term
stage
Definition
secures the glass slide
Term
compound microscope
Definition
contains more than one lens, bends light
Term
Total magnification formula
Definition
MAGtot=MAGobj X MAGocu
Term
parfocal
Definition
the image will remain nearly focused after the 40X objective lens is moved into place
Term
parcentered
Definition
the image will remain centered in the field of view after the 40X objective lens is in place
Term
fine adjustment knob
Definition
changes the specimen-to-objective distance
Term
field of view
Definition
the area that you can see through the ocular and objective
Term
ocular micrometer
Definition
small glass disk with thin lines numbered and etched in a row
Term
stage micrometer
Definition
a glass slide having precisely spaced lines etched at known intervals
Term
FOV to MAG formula
Definition
FOVlow X MAGlow=FOVhi X MAGhi
Term
FOVlow
Definition
diameter of the field of view of the low-power objective
Term
MAGlow
Definition
magnification of the low-power objective
Term
FOVhi
Definition
diameter of the field of view of the high-power objective
Term
MAGhi
Definition
magnification of the high-power objective
Term
depth of field
Definition
the thickness of the object in sharp focus
Term
dissecting (stereoscopic) microscope
Definition
used to view objects that are opaque or too large to see with a compound microscope; larger work distance; allows to dissect and manipulate specimens; large depth of field
Term
random motion
Definition
passively moves molecules in biological systems caused by heat
Term
Brownian movement
Definition
visible with high magnification; when particles are small enough to vibrate when other molecules bump into them
Term
diffusion
Definition
when substances move through solutions and across membranes in a predictable direction
Term
direction of diffusion
Definition
depends on the presence of a gradient of concentration, heat, and pressure. Molecules diffuse from an area of high concentration, heat, and pressure to an area of low concentration, heat and pressure.
Term
rate of diffusion
Definition
determined by the steepness of the gradient, it's size, polarity or solubility.
Term
differentially permeable
Definition
when a membrane allows some molecules to pass while retarding others
Term
polar molecules
Definition
positively charged areas and negatively charged areas
Term
two characteristics of molecules that govern their passive movement through a lipid membrane
Definition
polarity and size
Term
nonpolar molecules
Definition
no local areas of positive or negative charge
Term
dialysis
Definition
the separation of dissolved substances by means of their unequal diffusion through a differentially permeable membrane
Term
Phenolphthalein
Definition
is a pH indicator that runs red in basic solutions
Term
Iodine
Definition
a starch indicator that turns from yellow to dark blue in the presence of starch
Term
Osmosis
Definition
Diffusion of water across a differentially permeable membrane
Term
solution
Definition
homogeneous liquid mixture of two or more kinds of molecules
Term
solvent
Definition
fluid that dissolves substances
Term
solute
Definition
substance dissolved in a solution
Term
hypotonic
Definition
solution of lower concentration of solutes (cell swells)
Term
hypertonic
Definition
solution with a high concentration of solutes (cell shrinks)
Term
Isotonic
Definition
equal concentrations of solutes(remains the same)
Term
dependent variable
Definition
graphed on vertical axis
Term
independent axis
Definition
graphed on horizontal axis
Term
water potential
Definition
combined effects of concentration and pressure
Term
lysis
Definition
destruction of a cell by the influx of water (causing it to burst)
Term
hemolysis
Definition
destruction of a red blood cell
Term
plasmolysis
Definition
the shrinking of the cytoplasm of a plant cell in response to diffusion of water out of the cell and into hypertonic solution(high salt concentration) surrounding the cell
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