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Biology Chapter 6
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29
Biology
Undergraduate 1
09/30/2012

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Term
magnification
Definition
the ratio of an object’s image size to its actual size
Term
magnification, resolution & contrast
Definition
elements of microscopy
Term
resolution
Definition
measure of the clarity of an image as a result of magnification
Term
resolution
Definition
the minimum distance two points can be separated and still be distinguished as
two unique points
Term
contrast
Definition
measure of the distinguishability of different parts of a sample
Term
contrast
Definition
scientists stain parts of samples different colors to increase this
Term
light microscope, scanningelectron microscope , transmission electron microscope
Definition
types of microscopes (3)
Term
light microscope (LM)
Definition
passes visible light through a specimen and then through glass lenses which
refract the light and magnify the image
maximum magnification: 1000×
resolution limit: 200 nanometers
Term
scanningelectron microscope (SEM)
Definition
focuses a beam of electrons across the surface of a specimen
gives a 3-dimensional image of the surface of the specimen
can only be used on dead or non-living samples
resolution limit: 2 nanometers – 0.002 nanometers
Term
transmission electron microscope (TEM)
Definition
focuses a beam of electrons through a specimen
gives extremely detailed images of the interior of cells
can only be used on dead or nonliving specimens
resolution limit: 2 nanometers – 0.002 nanometers
Term

EMs

  • more detailed
  • allow to see within a cell's interior
  • allow to see outer surface

LMs

  • cheaper
  • faster to use
  • observe living cells

 

Definition

What is the difference between Light Microscopes and Electron Microscopes?

 

Term
cell fractionation
Definition
separates major cell organelles and subcellular structure to be studied
Term
centrifuge
Definition
an instrument that spins samples at extreme speeds to separate the contents of the cell
Term
prokaryotes & eukaryotic cells
Definition
types of cells
Term
prokaryotes
Definition
defined by the lack of internal membrane structures
typically smaller than eukaryotes
exist as relatively simple unicellular organisms (simpler than eukaryotes, but still
complex enough for life)
includes: bacteria and archaebacteria
Term
eukaryotic
Definition
defined by the presence of internal membrane structures
large, complex cells
can possibly form multicellular organisms
includes: animals, plants, fungi, and protists
Term
internalmembrane structures
Definition
Note: Current debate ensues on how to classify protists, and some scientists do
not consider them eukaryotes, but they do have
Term
domains of
organisms
Definition
Note: Bacteria and archaebacteria are two completely different
Term
plasma membrane
Definition
outer membrane present for ALL cells (both eukaryotes and prokaryotes); internal
membrane structures only exist in eukaryotes (These notes apply to all membranes, not
just the outer membrane.)
Term
plasma membrane
Definition
composed of the lipid bilayer
Term
lipid bilayer
Definition
phospholipids with hydrophobic fatty acid tails and hydrophilic phosphate heads
Term
a semi-permeable membrane
Definition
certain small molecules can diffuse through
larger molecules can enter through specific membrane protein channels
Term
concentration gradients
Definition
molecules tend to move from areas of higher concentration to areas of lower
concentration
membranes can be used to establish these areas of concentration and either maintain
or change them
Term
glycoproteins
Definition
membrane embedded protein with a carbohydrate attached to it
used in cell-to-cell communication
Term
cholesterol
Definition
can increase fluidity of a membrane
Term
unsaturated fats
Definition
are more fluid than saturated fats because of the kink in the fatty
acid chain
Term
embedded proteins
Definition
plasma membranes contain copious amounts of
Term
“fluid” because individual molecules that make up the protein move
around; “mosaic” because membrane is made of a collection of phospholipids,
proteins, and other large molecules
Definition
how does the fluid mosaic model get its name?
Term
maximum efficiency
Definition
occurs at a maximum surface area to volume ratio
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