A recombinant DNA molecule is:

a single DNA molecule made from two different sources

Which of the following is NOT a necessary part of a plasmid?

a.) telomeric ends

 b.) restriction sites

      c.) origin of replication

    d.) selectable marker

a.) telomeric ends

Which of the following is an example of a DNA palindromic sequence?

a.) AGTC

    b.) AAGGAT

   c.) AAGGTT

  d.)TATAGA

c.) AAGGTT

Gel electrophoroesis seperates DNA fragments by:

pulling negatively charged DNA towards the negative electrode

Can travel further through the gel matrix

DNA ligase can join 2 fragments by:

Catalyzing the formation of a phosphodiester bond

What is the correct order in PCR?

  a.) Synthesis ---> Annealing ---> Denaturation

b.) Denaturation --> Annealing ---> Synthesis

         c.) Annealing ---> Denaturation ---> Annealing ---> Denaturation

 d.) Annealing ---> Denaturation ---> Synthesis

b.) Denaturation --> Annealing ---> Synthesis

How is the DNA unwond in a OCR expiriment?

High temperature

Which of the following is NOT an example of a use for PCR?

 a.) Preperation of DNA fingerprints from crime scene DNA

  b.) Detection of genetic defects from a single embryo cell

    c.) Increasing transformation of non-competent e. coli species

d.) Analysis of data from extinct species like Homo  neandertalensis

c.) Increasing transformation of non-competent e. coli species

A restriction site is:

A specific sequence of DNA recognized by a restriction endonuclease

Restriction enzymes originate from_____ .

Bacterial cells

Why are antibiotic resistant markers important componets of cloning vectors?

They allow identification of bacteria that have been taken up the vector

The polymerase chain reaction (PCR) includes all of the following steps EXCEPT _____.

           a.) heating of the solution to break apart DNA strands

           b.) addition of new Taq polymerase after each cycle

            c.) annealing of primers to the complementary sequnces on the DNA

        d.) primer extension with a DNA polymerase

b.) addition of new Taq polymerase after each cycle

Why is this particular biotechnology called DNA fingerprinting?

The pattern of DNA fragments is as unique as fingerprints

Which of the followin best describes why genetic material engineering is possible?

a.) All living organisims make the same proteins

b.) All living organisms have the same genetic code

c.) All living organisms have the same restriction enzymes

d.) all living organisms posses plasmids

b.) All living organisms have the same genetic code

In order to make a successful subunit vaccine you need:

Active pathogen

Gene therapy involves_____.

Adding functioning version of a defective gene to the cells of an individual.

What is a recombinate DNA molecule?

A single DNA molecule made from 2 different sources. began in mid 1970

What is an enzyme? In what way is recombinant DNA technology based "on enzymes that can be used to manipulate DNA"?

Enzymes called restriction endonuclease revolutionized molecular biology because of their ability to cleave DNA at specific sites

What does nuclease enzyme do?

They degrade DNA. If a DNA sequence were a rope, then restriction enzymes would be a knife that always cut the rope into specific peices

What is a restriction endonuclease?

Enzymes with the ability to cleave DNA at specific sites

Does a resriction site cut randomly or at specific locations?

Specific locations

What is a restriction site?

Where enzymes cleave DNA