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Biology 1113 Final Study Guide
Final Exam Study Guide
158
Biology
Undergraduate 2
04/24/2013

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Term
Isolation of a plasmid from a bacterium
Definition

occurs first in the production of a recombinant plasmid

 

Term
You can use PCR to increase the amount of DNA available for restriction fragment analysis.
Definition
When linking an individual to a crime with only one tiny drop of blood as evidence, how can use this drop of blood to make the association?
Term
Cloned & Sequenced
Definition
To find the necleotide sequence of human chromosomes, they had to be digested into small fragments then_____.
Term
TAAGC
Definition
binds with a DNA fragment with a stickly end that reads -AATCG
Term
The same restriction enzyme is used to isolate the gene of interest and to cut the plasmid DNA.
Definition

step that occurs earliest in the process of producing recombianant DNA

 

Term
98%
Definition
the percentage of the human genome consists of noncoding DNA
Term
Proteomics
Definition
Study of full protein sets that genomes encode
Term

Genetically Modified Organism

 

Definition
an organism carrying a gene that was acquired by artificial means
Term
Humans & Chimps share a relatively recent common ancestor.
Definition
Statement that can be logically inferred from the amount of DNA shared by humans and chimps.
Term
A restriction enzyme
Definition
"sticky ends" are produced as a result of the action of___
Term
Sticky Ends
Definition
single-stranded ends of fragments of double-stranded DNA
Term
genomic library
Definition
collection of cloned recombianant plasmids that includes fragments from the entire genome of a cell
Term
transgenic animal
Definition
an animal containing a gene from another species
Term
whole-genome shotgun approach
Definition
technique most commonly used to sequence entire genomes
Term
2n-1
Definition
number of chromosomes that an individual with Turner syndrome has
Term
genetic recombination
Definition
crossing over during prophase I results in this
Term
XY
Definition
sex chromosomes belonging to a normal human male
Term
homologous chromosomes randomly separate and migrate to opposite poles
Definition
Best description of events in anaphase I
Term
During telophase
Definition
the evetns of prophase are reversed
Term
n+1 or n-1
Definition
how many chromosomes can a gamete possess as a result of nondisjunction?
Term
During metaphase I
Definition
homologous chromosomes line up in the middle of a cell
Term
Cell Cycle
Definition
results in the production of two cells, each with the same amount of genetic material and the same genetic information
Term
chromosomes
Definition
DNA is found in these structures
Term
The mitotic spindle begins to form
Definition
during prophase
Term
telophase
Definition
cytokinesis typically occurs during the ____ stage of mitosis
Term
one difference between mitosis and meiosis
Definition
mitosis produces cells genetically identical to the parent cell, but meiosis does not
Term
Anaphase II is essentially the same as mitotic anphase except that in anaphase II_____  and in mitoric anaphase _____.
Definition

the cells are haploid and sister chromatids separate...

the cells are diploid and sister chromatids separate

Term
44
Definition
number of autosomes  humans have
Term
mutation of homeotic genes
Definition
most likely to cause the development of a six-legged frog
Term
Tobacco
Definition
responsble for more cancers than any other carcinogen
Term
the cells exhibit different patterns of gene expression
Definition
how is it that cells in different body tissues are able to perform different functions?
Term
cell division
Definition
many proto-oncogenes regulate_____
Term
tumor-suppressor gene
Definition
data suggest that the normal version of BRCA1 functions as a ________.
Term
lung cancer
Definition
More people die of this cancer than any other.
Term
attachment of RNA polymerase to the promoter
Definition
plays a role in the regulation of transcription in both prokaryotic an deukaryotic cells
Term

embryonic stems cells are undifferentiated; adult stem cells are partially differentiated

 

Definition
difference between embryonic and adult stem cells?
Term
operator
Definition
acts as an on/off switch in an operon
Term
came from a normal human female
Definition
human cell that functions normally, has 45 functional chromosomes and one chromossome that is almost completely inactive
Term
oncogene
Definition
name given to a gene that causes cancer
Term
operon
Definition
production o fa single RNA transcrip for a group of related genes in under the control of this
Term
predisposition to these cancers is inherited
Definition
inhertance of certain genes increases the risk of getting certain cancers so it can be said that___
Term
Systematics
Definition
study of the evolutionary relationships of organisms, past and present
Term
explosive diversification
Definition
period of mass extinction is often followed by this
Term
reduced hybrid viability
Definition
Rana pipiens and Rans sylvatica frogs mate and the ofspring die early in embryonic development
Term

example of paedomorphosis

 

Definition
the ability to reproduce evolving in caterpillars
Term
Creataceous
Definition
When dinosaurs (aside from the lineage that produced birds) were extinct by the end of this era
Term
its appearance
Definition
fossil species is distringuished mainly by
Term
sapiens
Definition
species name
Term

basis on which populations are assigned to the same biological species

 

Definition
being able to interbreed and produce fertile offspring
Term
class
Definition
most inclusive taxonomic level
Term
homologous to
Definition
the wing of a bald eagle is ____ to the wing of a penguin.  this means that even though both are wings, they do not serve the same purpose on the birds.
Term
geographic isolation
Definition
sympatric speciation specific excludes this
Term
equilibrium
Definition
you notice that through time a series of fossils exhibits very little change, this fossil lineage can be described as exhibiting______.
Term
bacteria that only reproduce assexually
Definition
biological specials concept cannot be applied to this
Term
punctuated equilibrium
Definition
pattern of evolution in which most change in appearance takes place during a realtively short period of time fits the ______ model of speciation.
Term
promoter
Definition
region of DNA where RNA synthesis begins
Term
one
Definition
smallest number of necleotides that must be added or subtracted to change the triplet grouping of the genetic message
Term
After replication
Definition
each new DNA double helix consists of one old strand and one new strand
Term
transcription
Definition
manufacture of a strand of RNA complementary to a strand of DNA
Term
uracil ...... thymine
Definition
RNA contains the nitrogenous base ____ instead of ____ which is only found in DNA.
Term
three
Definition
number of nucleotides that make up a codon
Term
Watson & Crick
Definition
discovered the structure of DNA
Term
DNA double helix is
Definition
made up of two polynucleotide strands
Term
mutation within a gene that will insert a premature stop codon in mRNA would
Definition
result in a shortened polypetide chain
Term
bacteriophages
Definition
viruses that infect bacteria
Term
ribosomes
Definition
where translation is accomplished
Term

prophage

 

Definition
bacteria
Term
provirus
Definition
animal cells
Term
backbone of DNA
Definition
a repeating sugar-phosphate-sugar pattern
Term
messenger RNA or mRNA
Definition
RNA that is translated into a polypeptide
Term
Reverse transcriptase
Definition
HIV must use it's own_____ to reproduce.
Term
1:1:1:1
Definition
Expected phenotypic ratio of a cross between an RrYy and an rryy individual
Term
pleiotrophy
Definition
the inheritance of Marfan syndrome is an example of this
Term
Linked
Definition
genes violate Mendel's principle of independt assortment
Term
true-breeding plant is one that
Definition
self-fertilizes to produce offspring identical to the parent
Term
Key to recognition of incomplete dominance
Definition
the phenotype of the heterozygote falls between the phenotypes of the homozygotes
Term
0%
Definition
Probability of a couple who has two femal children that their next child will be male
Term
A Carrier
Definition
an individual that is heterozygous for cystic fibrosis
Term
Basis of Mendel's Laws
Definition
the behavior of chromosomes during metaphase I and anaphase I of meiosis
Term

dominant

 

Definition
the allele for purple flowers produced when mendel crossed true-breeding purple-flowered plants with true-breeding white flowered plants and all offspring produced purple flowers.
Term
He can't (unless there is a mutation.)
Definition
How can a man with normal color vision father a daughter who is red-green color-blind?
Term
Key to the recognition o fcodominance
Definition
the heterozygote expresses the phenotype of both homozygotes
Term
The trait varies along a coninuum in the population
Definition
key to the recognition of a trait whose expression is determine dby the effects of two or more genes (polygenic inheritance)
Term
An example of disruptive selection
Definition
Garter snakes with different coloration pattersn behave differently when threatened.
Term
reproductive success
Definition
Darwinian fitness measures this
Term
3.5 billion
Definition
oldest known fossils
Term
hardy-Weinberg formula; p2 represents
Definition
frequency of homozygous dominants
Term
bones of extinct whales
Definition
component of the fossil record
Term
example of sexual selection
Definition
Peahens choose to mate with peacocks that have the most beautiful tails.
Term
0.30
Definition
frequency of one allele in a population is 0.7, so the frequence of the alternate allele is _____
Term
Most likely to apply to a populatoin that survives a bottleneck and recovers to a point where it consists of as many individuals as it did prior to the bottleneck.
Definition
the post bottlenect population exhibits less genetic variation than the prebottleneck population.
Term
True
Definition
natural selection works on a variation already present in a population
Term
Genetic drift is the result of
Definition
chance
Term
64%
Definition
Percentage of individuals in a population that are homozygous dominant for freckles if four percent of the individuals lack freckles and you use the Hardy-Weinberg formula to calculate
Term
Which is a population?
Definition
the termites infesting your house
Term
While on Beagle, Darwin was influence dby a book by Charles Lyell that suggested that Earth was _____ and sculpted by geologic processes that ____ today.
Definition
old......continue
Term
Palentology
Definition
study of fossils
Term
Increase in antibiotic reseistant strains of bacteria
Definition
example of directional selection
Term
50%
Definition
Percentage of probability of having a child with attached earlobes  when and individual mates with an indivdual heterozygous for free earlobes
Term
key to the recognition of incomplete dominance
Definition
the phenotype of the heterozygote falls between the phenotypes of the homozygotes
Term
Probability of a three-nostrilled man's grandson having three nostrils
Definition
0%
Term
influenced by both genes and the environment
Definition
many human traits, such as performance on intelligence tests or our susceptibility to heart disease are ___
Term
Alleles
Definition
alternate versions of a gene
Term
Linked Genes
Definition
violate Mendel's principle of independent assortment
Term
DBC
Definition
The recombination frequency between gene B and gene C is 11%.  the recombination frequencey between gene B and gene D is 5%.  the recombination frequency between gene C and gene D is 15%.  What would be the  arrangement of these genes on a linkage map?
Term
hybrid
Definition
mating between a true-breedin purple flowered pea plant and a true-breeding white flowered pea plant would produce this
Term
basis of Mendel's Laws
Definition
the behavior of chromosomes during metaphase I and anaphase I of meiosis
Term
0%
Definition
If an individual with wavy hair mates with and individual with straign hair, what is the probability that their child will have curly hair?
Term
gametes have one copy of each allele
Definition
according to mendel's law of segregation
Term
21000
Definition
the human genome contains approximately this many genes.
Term
Cutting DNA with a particular restriction enzyme producse DNA framents tha can be separated by
Definition
gel elecrophoresis
Term
desired gene is inserted into the plamis nad the plasmid is taken up the bacterium
Definition
when plasmids are use dto produce a desired protein
Term
restriction enzymes
Definition
a DNA sample is treated with this to make restriction fragments
Term
humulin
Definition
the world's first genetically engineered pharaceutical product
Term
DNA ligase
Definition
used to bind DNA fragments together
Term
Genetically modified organism
Definition
an organism carrying a gene that was acquried by artificial means
Term

whole-genome shotgun approach

 

Definition
technique most commonly used to sequence entire genomes
Term
cloned and sequenced
Definition
chromosmomes had to be digested into small fragments and then _____
Term
same restricitn enzyme is used to isolate the gene of interest and to cut the plasmid DNA
Definition
occurs earlies in the proces of producing recombianant DNA
Term
using DNA ligase to join DNA fragments
Definition
last step in the production of a recobianant DNA plasmid
Term
isolation of a plasmid from a bacterium
Definition
occurs first in the production of a recomibinant plasmid
Term
Correct order of the stages of translation
Definition
initiation, codon recognition, peptide bond formation, translocation, termination
Term
phenotype
Definition
name given to the collection of traits exhibited by an organism
Term
RNA polymerase
Definition
enzyme responsible for RNA synthesis
Term
mutation
Definition
ultimate source of all diversity
Term
DNA double helix
Definition
is made up of two polynucleotide strands
Term
the absence of a terminator in transcription will result in
Definition
the production of a longer RNA molecule
Term
How can bacteriophage DNA be spread from cell to cell without causing cell death?
Definition
via a lysogenic cycle
Term
A mutation within a gene that will insert a premature stop codon in mRNA would result in a
Definition
shortened polypeptide chain
Term
three
Definition
number of nucleotides that make up a codon
Term
exons
Definition
exptessed (coding)regions of eukaryotic genes
Term
backbone of DNA
Definition
repeating sugar-phosphate-sugar-phosphate pattern
Term
an exaptation
Definition
switch in function
Term
1.4 million years old
Definition
How old is the age of a rock sample if it is determeined to have one quarter of the uranium 235 content it had when it formed?
Term
pre-zygotic barrier
Definition
a reproductive barrier that prevents species from mating is an example of _____.
Term
Glycolysis-Citric Acid Cycle-ETC
Definition
Stages of cellular respiration
Term
Starch
Definition
iodine turns dark blue in the presence of this
Term
Interphase
Definition
first stage of mitosis
Term
human-lizard-bird
Definition
all have a radius an dulna similar to the common ancestor
Term
Compound Microscope
Definition
has two sets of lenses
Term
The primary photysynthetic pigment is cartoene
Definition
false
Term
Conclusion
Definition
step in the scientific method that follows experiments and observations
Term
diffusion
Definition
movement of molecules from an area o fhigher concentration to one of lower concentration
Term
glucose
Definition
both cellular respiration and fermentation begin with this molecule
Term
G1-S-G2-M-Cytokinesis
Definition
correct order for cell cycle
Term
False
Definition
only plants are capable of cellular respiration
Term
G
Definition
complementary base pair rules say that C pairs with
Term
The Rf for carotene can be determined by dividing the distance the yellow orange pigment (cartene) migrated by the distance the solvent front migrated.
Definition
Considering the chromotograme you completed in the lab bench exercise, the following is true
Term
hypothesis
Definition
tenative explanation of observed phenomena
Term
Both cellular respiration and fermentation provide what molecule for the cell?
Definition
ATP
Term
The potassium hydroxide is used to remove ____ as it is given off.
Definition
carbon dioxide
Term
Interphase
Definition
DNA is in the form of chromatin during this stage of the cell cycle.
Term
Prophase
Definition
During this stage of mitosis, the nuclear envelope disappears and the DNA condenses into chromosomes.
Term
Metaphase
Definition
duplicated chromosomes line up in the middle of the cell during this stage
Term
Anaphase
Definition
sister chromatids pull apart during this stage
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