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Biology 11 12 13
Covers Nucleic Acids, Gene Expression, and Gene Regulation
55
Biology
Undergraduate 1
10/14/2010

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Term
Key criteria genetic material must meet
Definition
Information, replication, transmission, and variation
Term
Information
Definition
The genetic material must contain the information necessary to construct an entire organism.
Term
Replication
Definition
The genetic material must be accurately copied.
Term
Transmission
Definition
After it is replicated, the genetic material can be passed from parent to offspring. It also must be passed from cell to cell during the process of cell division.
Term
Variation
Definition
Differences in the genetic material must account for the known variation within each species and among different species.
Term
Transformation
Definition
A type of genetic transfer between bacteria in which a segment of DNA from the environment is taken up by a competent cell and incorporated into the bacterial chromosome.
Term
Bacteriophage (phage)
Definition
A virus that infects bacteria.
Term
Nucleic acid
Definition
An organic molecule composed of nucleotides. The two types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)
Term
Levels of complexity of DNA's structural features
Definition
Nucleotides, strand, double helix, chromosomes, and genome
Term
Nucleotide
Definition
An organic molecule having three components: one or more phosphate groups, a five-carbon sugar (either deoxyribose or ribose), and a single or double ring of carbon and nitrogen atoms known as a base.
Term
Strand
Definition
A structure of DNA (or RNA) formed by the covalent linkage of nucleotides in a linear manner.
Term
Double helix
Definition
Two strands of DNA hydrogen-bonded with each other. In a DNA double helix, two DNA strands are twisted together to form a structure that resembles a spiral staircase.
Term
Chromosome
Definition
A discrete unit of genetic material composed of DNA and associated proteins. Eukaryotes have chromosomes in their cell nuclei and in plastids and mitochondria.
Term
Genome
Definition
The complete genetic composition of a cell or a species.
Term
Three components of a nucleotide
Definition
A phosphate group, a pentose sugar, and a nitrogenous base.
Term
Purines
Definition
The bases adenine (A) and guanine (G), with double rings of carbon and nitrogen atoms.
Term
Pyrimidines
Definition
The bases thymine (T), cytosine (C), and uracil (U) with a single ring of carbon and nitrogen atoms.
Term
Phosphodiester linkage
Definition
Refers to a double linkage (two phosphoester bonds) that holds together adjacent nucleotides in DNA and RNA strands.
Term
Backbone
Definition
The linear arrangement of phosphates and sugar molecules in a DNA or RNA strand.
Term
Directionality
Definition
In a DNA or RNA strand, refers to the orientation of the sugar molecules within that strand. Can be 5' to 3' or 3' to 5'.
Term
Base pairs
Definition
The structure in which two bases in opposite strands of DNA hydrogen-bond with each other.
Term
AT/GC rule
Definition
Refers to the phenomenon that an A in one DNA strand always hydrogen-bonds with a T in the opposite strand, and a G in one strand always bonds with a C.
Term
Complementary
Definition
Describes the specific base pairing that occurs between strands of nucleic acids; A only pairs with T (in DNA) or U (in RNA), and G only pairs with C.
Term
Antiparallel
Definition
The arrangement in DNA where one strand runs in the 5' to 3' direction while the other strand is oriented in the 3' to 5' direction.
Term
Groove
Definition
In the DNA double helix, an indentation where the atoms of the bases make contact with the surrounding water.
Term
Major groove
Definition
A groove that spirals around the DNA double helix; provides a location where a protein can bind to a particular sequence of bases and affect the expression of a gene.
Term
Minor groove
Definition
A smaller groove that spirals around the DNA double helix.
Term
DNA replication
Definition
The process by which DNA is copied.
Term
Daughter strand
Definition
The newly made strand in DNA replication.
Term
Parent strand (template strand)
Definition
The original strand in DNA replication.
Term
Three proposed mechanisms for DNA replication
Definition
Semiconservative mechanism, conservative mechanism, dispersive mechanism
Term
Semiconservative mechanism
Definition
The correct model for DNA replication; double-stranded DNA is half conserved following replication, resulting in new double-stranded DNA containing one parental strand and one daughter strand.
Term
Conservative mechanism
Definition
In this incorrect model for DNA replication, both parental strands of DNA remain together (are conserved) following DNA replication. The two newly made daughter strands also occur together.
Term
Dispersive mechanism
Definition
In this incorrect model for DNA replication, segments of parental DNA and newly made DNA are interspersed in both strands following the replication process.
Term
Origin of replication
Definition
A site within a chromosome that serves as a starting point for DNA replication.
Term
Replication fork
Definition
The area where two DNA strands have separated and new strands are being synthesized.
Term
Bidirectional replication
Definition
The process in which DNA replication proceeds outward from the origin in opposite directions.
Term
DNA helicase
Definition
An enzyme that uses ATP to separate DNA strands during DNA replication.
Term
DNA topoisomerase
Definition
An enzyme that alleviates DNA supercoiling during DNA replication.
Term
Single-strand binding protein
Definition
A protein that binds to both of the single strands of parental DNA and prevents them from re-forming a double helix during DNA replication.
Term
DNA polymerase
Definition
An enzyme responsible for covalently linking nucleotides together during DNA replication.
Term
Deoxynucleotide triphosphates
Definition
Individual nucleotides with three phosphate groups.
Term
DNA primase
Definition
An enzyme that synthesizes a primer for DNA replication.
Term
Primer
Definition
A short segment of RNA, typically 10 to 12 nucleotides in length, that is needed to begin DNA replication.
Term
Leading strand
Definition
During DNA replication, a DNA strand made in the same direction that the replication fork is moving. The strand is synthesized as one long continuous molecule.
Term
Lagging strand
Definition
During DNA replication, a DNA strand made as a series of small Okazaki fragments that are eventually connected to each other to form a continuous strand.
Term
Okazaki fragments
Definition
Short segments of DNA synthesized in the lagging strand during DNA replication.
Term
DNA ligase
Definition
An enzyme that catalyzes the formation of a covalent bond between nucleotides in adjacent DNA fragments to complete the replication process.
Term
Proofreading
Definition
The ability of DNA polymerase to identify a mismatched nucleotide and remove it from the daughter strand.
Term
Telomere
Definition
A region at the ends of eukaryotic chromosomes where a specialized form of DNA replication occurs.
Term
Telomerase
Definition
An enzyme that catalyzes the replication of telomere.
Term
Senescent
Definition
Cells that have doubled many times and have reached a point where they have lost the capacity to divide any further.
Term
Chromatin
Definition
Refers to the biochemical composition of chromosomes, which contain DNA and many types of proteins.
Term
Histones
Definition
A group of proteins involved in the formation of nucleosomes that aid in the compaction of eukaryotic DNA.
Term
Nucleosome
Definition
A structural unit of eukaryotic chromosomes composed of an octamer of histones (eight histone proteins) wrapped with DNA.
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