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Biology 107 Exam 2 WSU
Biology 107 Exam 2 WSU( Washington State University ) Dr. Helmick
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Biology
Undergraduate 1
03/08/2011

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Term
ATP
Definition
(adenosine triphosphate) Functional Group. An adenine containing nucleoside triphosphate that releases free energy when its phosphate bonds are hyrdrolyzed. This energy is used to drive endergonic reactions in cells.
Term
Endergonic Reaction
Definition
A non-spontaneous chemical reaction, in which free energy is absorbed from the surroundings.
Term
Kinetic Energy
Definition
Energy associated with the relative motion of objects.
Term
Potential Energy
Definition
The energy that matter posses as a result of its location or spatial arrangement (structure).
Term
Enzymes
Definition
A macromolecule serving as a catalyst, a chemical agent that increases the rate of a reaction without being consumed by the reaction. Most enzymes are proteins. Enzymes are catalysts (but catalysts are not enzymes).
Term
pH optimum
Definition
The pH at which an enzyme has maximum potency and efficiency.
Term
Temperature optimum
Definition
The temperature at which any operation, such as the culture of any special microorganism, is best carried on.
Term
Competitive Inhibitor
Definition
A substance that reduces the activity of an enzyme by entering the active site in place of the substrate, whose structure it mimics.
Term
Noncompetitive Inhibitor
Definition
A substance that reduces the activity of an enzyme by binding to a location remote from the active site, changing the enzyme's shape so that the active site no longer effectively catalyzes the conversion of the substrate to product.
Term
Feedback Inhibition
Definition
A method of metabolic control in which the end product of a metabolic pathway acts as an inhibitor of an enzyme within that pathway.
Term
Metabolic Pathway
Definition
A series of chemical reactions that either builds a complex molecule (anabolic pathway) or breaks down a complex molecule to a simpler molecule (catabolic pathway)
Term
Substrate
Definition
The reactant on which an enzyme works.
Term
Active Site
Definition
The specific region of an enzyme that binds the substrate and that forms the pocket in which catalysis occurs.
Term
The structure of ATP. In the cell, most hydroxyl groups of _________ are ________.
Definition
Phosphates, ionized.
Term
The hydrolysis of ATP. The reaction of ATP and water yields inorganic _____________ and _________ and releases ______________.
Definition
phosphate, ADP, energy
Term
Hydrolysis
Definition
A chemical reaction in which the interaction of a compound with water results in the decomposition of that compound.
Term
Transport work: ATP phosphorylates ________ _________.
Definition
Transport Proteins.
Term
Mechanical Work: ATP binds ____________ to _________ and then is hydrolyzed
Definition
noncovalently, motor proteins
Term
Phosphorylated Intermediate
Definition
A molecule (often a reactant) with a phosphate group convalently bonded to it, making it more reactive (less stable) than the unphosphorylated molecule.
Term
ATP cycle
Definition
Energy released by breakdown reactions (catabolism) is used to phosphorylate ADP, regenerating ATP. Chemical potential energy stored in ATP drives most cellular work.

ATP+H2O (hydrolysis) -->
ADP + Phosphorus and releases energy -->
energy from catabolism gained + ADP +P -->
ATP (+ H20).....
Term
Active Site and Catalyst Cycle of an Enzyme
(6 Steps)
Definition
1) Substrates enter active site, enzyme changes shape to make substrates fit nicely (like a puzzle).
2) Substrates held in active site by weak interactions (hydrogen/ionic bonds).
3) Active site speeds up reaction.
4) Substrates converted to products.
5) Products released.
6) Active site is available for 2 new substrates.
Term
Fermentation
Definition
A catabolic process that is a partial degradation of sugars or other organic fuel that occurs without the use of oxygen
Term
Cellular Respiration
Definition
The most efficient way for cells to harvest energy stored in food is through this. The catabolic pathways of aerobic and anaerobic respiration, which breaks down organic molecules and use an electron transport chain for the production of ATP.
Term
Cytoplasm
Definition
Semi fluid material surrounding organelles; site of many cellular reactions (cellular respiration)
Cytoplasm offers support to the cell. It allows the cell to take up 3-dimensional space and the cell's many organelles to "float" freely throughout. Also acts as a medium for transport inside the cell.
The cytoplasm is a liquid in which the organelles float in. It speeds up intercellular travel.
Term
Mitochondria and ATP production.
Definition
The electron transport "chain" is a series of electron carriers in the membrane of the mitochondria in eukaryotic cells. Through a series of reactions, the "high energy" electrons are passed to oxygen. In the process, a gradient is formed, and ultimately ATP is produced.
Term
Oxidation and Reduction
Definition
OIL (or) RIG of electrons.
Term
Electron Transport Chain
Definition
Consists of a number of molecules, mostly proteins, built into the inner membrane of the mitochondria of eukaryotic cells and the plasma membrane of aerobically respiring prokaryotes.
Term
Pyruvate
Definition
An organic acid that is a product of the split sugars in glycolysis.
Term
Glycolysis
Definition
iterally means "splitting sugars." Glucose, a six carbon sugar, is split into two molecules of a three carbon sugar. In the process, two molecules of ATP, two molecules of pyruvic acid and two "high energy" electron carrying molecules of NADH are produced. Glycolysis can occur with or without oxygen. In the presence of oxygen, glycolysis is the first stage of cellular respiration. Without oxygen, glycolysis allows cells to make small amounts of ATP. This process is called fermentation.
Term
Krebs Cycle begins after the two molecules of the three carbon sugar produced in glycolysis are converted to a slightly different compound.

What is that compound?
Definition
Acetyl CoA
Term
ATP Synthase
Definition
A general term for an enzyme that can synthesize adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate by using a form of energy.
Term
Chemiosmosis
Definition
The diffusion of ions across a selectively-permeable membrane. More specifically, it relates to the generation of ATP by the movement of hydrogen ions across a membrane during cellular respiration. (Transfer of H+ across a membrane).
Term
How many ATP are in Glucose?
Definition
36
Term
How many ATP are in a triglyceride?
Definition
129
Term
Stages of Cellular Respiration
Definition
1) Glycolysis
2) Pyruvate oxidation and the citric acid cycle
3)Oxidative phosphorylation: electron transport and chemiosmosis
Term
Reactants and Products of Glycolysis
Definition
Reactants: Glucose
Products: pyruvate (ATP and electrons that are carried by NADH)
Term
During Pyruvate Oxidation, Pyruvate is converted to what?
Definition
Acetyl CoA
Term
In cellular respiration, what is the step after pyruvate oxidation?
Definition
Citric acid cycle
Term
Aldolase
Definition
cleaves the sugar molecule (during glycolysis) into 2 different 3-carbon sugars.
Term
__________ is reduced to ____________ in the Citric Acid Cycle.
Definition
NAD+, NADH
Term
Where is ATP Synthase located?
Definition
inner membrane of the mitochondria or the prokaryotic plasma membrane
Term
What is ATP Synthase?
Definition
the enzyme that actually makes ATP from ADP and inorganic phosphate.
Term
aka for citric acid cycle
Definition
krebs cycle
Term
stomata
Definition
microscopic pores that allows CO2 to enter the leaf and O2 to leave.
Term
Where is the chloroplast found?
Definition
Mesophyll
Term
Mesophyll
Definition
The tissue in the interior of the leaf.
Term
Approximately how many Chloroplasts are in the Mesophyll?
Definition
30-40
Term
Stroma
Definition
A dense fluid that is surrounded by an envelope in the chloroplast that had 2 members
Term
Thylakoids
Definition
A membrane-bound compartment inside chloroplasts and cyanobacteria. They are the site of the light-dependent reactions of photosynthesis. The word "thylakoid" is derived from the Greek thylakos, meaning "sac". ...
Term
Where is chlorophyll located?
Definition
The thylakoid membranes of the chloroplasts.
Term
What goes into the Calvin cycle?
Definition
ATP, NADPH, CO2
Term
What comes out of the Calvin Cycle?
Definition
CH2O (sugar), NADP+, ADP + P
Term
Light absorbed by chlorophyl drives a transfer of the electrons and hydrogen ions from water to an acceptor called what?
Definition
NADP+
Term
Visible light is between what range of wave length?
Definition
380 nm to 750 nm
Term
3 types of pigment in chloroplasts
Definition
Chlorophyll α, chlorophyll b, and carotenoids
Term
3 phases of Calvin Cycle
Definition
Carbon Fixation, Reduction, Regeneration of the CO2 acceptor
Term
What is the CO2 acceptor in the Calvin Cycle?
Definition
RuBP aka rubisco
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