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BIOL2416
iGenetics CH1-5 DNA Replication, Transcription Flashcards - also see CH6 Translation cards in Favorites below
41
Biology
Undergraduate 2
02/07/2009

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Term
test cross
Definition
1. cross of dominant phenotype with unknown genotype (either AA or Aa) with recessive.
2. Look at babies to figure out unknown parental genotype; recessive babies indicate Aa rather than AA.
Term
transcription
Definition
making RNA from DNA
Term
transcription - enzyme
Definition
RNA Polymerase
Term
RNA Polymerase II
Definition
for making mRNA
Term
transcription - location
Definition
nucleus
Term
RNA processing
Definition
1. eukaryotes ONLY
2. 5' cap, 3' polyA tail, splcing, NTP modification
Term
nucleotide components
Definition
1. phosphate group
2. pentose sugar
3. (nitrogenous) base
Term
DNA stands for
Definition
DeoxyriboNucleic Acid
Term
RNA stands for
Definition
RiboNucleic Acid
Term
DNA bases
Definition
GATC
Term
RNA bases
Definition
GAUC
Term
possible base pairs
Definition
AT
GC

AU
Term
strong covalent bond between adjacent nucleotides (within each strand)
Definition
phosphodiester bond
Term
weak bond between base pairs (velcro's 2 stands together)
Definition
H bond
Term
DNA structure
Definition
1. double helix
2. anti-parallel stands
strands composed of nucleotides phosphodiester-bonded together
4. 2 strands velcro'd together by H bonds
Term
DNA replication - definition
Definition
DNA synthesis during S phase of Interphase (before mitosis or meiosis)
Term
DNA replication - type of process
Definition
1. semi-cpnservative
2. bi-directional
3. semi-discontinuous
Term
semi-conservative
Definition
results in daughter DNAs that are half old and half new
Term
semi-discontinuous
Definition
1. leading starnd produces continuously (in one piece off 1 primer)
2. lagging strand produced in multiple Okazaki fragments off multiple primers
Term
Meselson-Stahl
Definition
showed semi-conservative DNA replication in Neurospora Crassa
Term
Griffith
Definition
1. Showed "transforming principle" in Streptococcus pneumonia
2. heat-killed S + live R kills mice
3. transforming principle probably not (heat-killed/denatured) protein
Term
deoxyribose
Definition
1. sugar in DNA
2. lacks O on #2 carbon
3. symbolized by d in dNTP
Term
nucleosome
Definition
1. DNA wound around histone proteins (bead)
2. eukaryotes only
Term
oriC function
Definition
Where DNA replication starts
Term
chromatin
Definition
chromosome material (histones + DNA)
Term
Okazaki fragments
Definition
covalently-bonded fragments that become the lagging strand
Term
covalent bond
Definition
1. shared pair of electrons
2. strong bond
3. inside molecules
Term
molecule
Definition
covalently bonded atoms
Term
hydrogen bond
Definition
1. weak bond
2. e.g. between nitrogenous bases
3. e.g. inside proteins (folding)
Term
phosphodiester bond
Definition
covalent bond between sugar of first nucleotide and phosphate of second nucleotide
Term
polarity
Definition
1. e.g DNA or RNA strands have a 5' beginning and a 3' end
2. e.g polypeptides have a N-terminus (amino) beginning and a C-terminal (carboxyl) end
Term
intron
Definition
1. spliced out of mRNA
2. not expressed into protein
Term
pre-mRNA or primary transcript
Definition
unprocessed mRNA
Term
RNA processing
Definition
1. 5' capping
2. 3' polyA tailing
3. Splicing
$. RNA editing

(Eukaryotes ONLY!!!)
Term
mature mRNA
Definition
1. ready for translation
2. no introns
3. has 5' cap and 3' tail
Term
translation
Definition
1. mRNA into polypeptide
2. by ribosomes
3. in cytosol
Term
protein
Definition
1. amino acids peptide-bonded together
2. folded up
Term
bi-directional DNA Replication
Definition
2 opposing forks moving away from OriC
Term
semi-conservative DNA Replication
Definition
daughter DNA has 1 parental + new strand
Term
antiparallel
Definition
2 strands running in opposite direction
Term
semi-discontinuous DNA Replication
Definition
continous on leading strand; discontinuous on lagging strand
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