Term
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Definition
| Inactive enzyme precursor. |
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Term
| How do you calculate the rate enhancement ratio? |
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Definition
| rate enhancement = rate catalyzed/rate uncatalyzed) |
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Term
| Serine proteases use what to catalyze the cleavage of peptide bond? |
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Definition
| Proximity and orientation, covalent catalysis, base catalysis, and electrostatic catalysis. |
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Term
| What the 'catalytic triad' in chymotrypsin consists of what? |
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Definition
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Term
| alpha-amylose has what type of conformation? |
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Definition
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Term
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Definition
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Term
| Bacterial cell walls are made of? |
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Definition
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Term
| Cellulose has what type of conformation? (structure) |
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Definition
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Term
| What is the Lineweaver-burk equation? |
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Definition
| 1/v0 = (Km/Vmax)(1/[s])+(1/Vmax) |
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Term
| What are all the catalytic mechanisms? |
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Definition
| Proximity and orientation, metal-ion, covalent catalysis, acid-base catalysis, and electrostatic catalysis. |
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Term
| The formation of Schiff base is a property of? |
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Definition
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Term
| Decarboxylation of acetoacetate produces? |
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Definition
| Schiff base via nucleophilic attack on substrate. |
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Term
| The catalytic mechanism of bovine pancreatic enzyme RNase A relies on acid-base catalysis involving which amino acid? |
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Definition
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Term
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Definition
| Acid donates protons lowering the free energy of the transition state |
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Term
| What type of amino acid does not provide a side-chain for acid-base catalysis? |
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Definition
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Term
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Definition
| A+B <=enzyme=> C+D+enzyme |
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Term
| Chymotrypsin relies on what to cleave a bulky non-polar side chain? |
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Definition
| Relies on its small hydrophobic side chains (its pocket). [Does not have charge, or sulfylhydral groups] |
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Term
| The step with the greatest delta G is the? |
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Definition
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Term
| Alcohol dehydrogenase catalyzes the conversion of ? |
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Definition
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Term
| How does competitive inhibition effect Km? |
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Definition
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Term
| How does uncompetitive inhibition effect Km? |
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Definition
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Term
| How does mixed inhibition effect Km? |
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Definition
| Decreases Vmax, or may (in/de)crease Vmax |
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Term
| What type of slope does uncompetitive inhibit exhibit? |
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Definition
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Term
| What type of slope does mixed inhibition exhibit? |
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Definition
| Intersection and shows divergent parallelism. |
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Term
| What type of slope does competitive exhibit? |
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Definition
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Term
| What does lysozyme catalyze? |
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Definition
| beta(1-4) glycosidic linkages |
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Term
| When do second-order reactions occur? |
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Definition
When two reactant collide, dawg.
Bimolecular. |
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Term
| What type of reaction is unimolecular? |
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Definition
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Term
| How do cells control or regulate the flux through metabolic pathways? |
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Definition
| Allosteric control of enzymes, covalent modification of enzymes, and genetic control of the concentrations of enzymes. |
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Term
| What type of reaction depends on its initial concentration? |
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Definition
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Term
| What compound has the highest oxidation number? |
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Definition
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Term
| Whats the second-order half-life equation? |
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Definition
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Term
| What is the first-order half-life? |
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Definition
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Term
| How is trypsinogen converted to trypsin? |
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Definition
| Via proteolysis of trypsinogen. |
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Term
| What is the Michaelis-Menten Equation for no inhibitor? |
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Definition
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Term
| What is the Michaelis-Menten Equation for competitive inhibitor? |
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Definition
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Term
| What is the Michaelis-Menten Equation for uncompetitive inhibitor? |
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Definition
| v0 = Vmax[S] / (Km+a'[S]) |
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Term
| What is the Michaelis-Menten Equation for mixed inhibitor? |
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Definition
| v0 = Vmax[S] / (aKm+a'[S]) |
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Term
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Definition
| The concentration of the substrate that half saturates the enzyme. |
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Term
| The cyclic five membered ring of sugar is... |
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Definition
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Term
| What is the measure of the catalytic efficiency of an enzymatic reaction? |
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Definition
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Term
| What allosteric effector of ACTase acts as an inhibitor? |
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Definition
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Term
| The ubiquitous chemistry of acetyl-CoA is centerered on... |
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Definition
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Term
| What is the oxidized and reduced forms of Flaven Adenine dinucleotide? |
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Definition
| FADH2, FAD (reduced, oxidized respectively) |
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Term
| When [S] = Km, what is v0=? |
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Definition
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Term
| What is the Keq equation? |
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Definition
| Keq = e ^ (-deltaG / RT ) |
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Term
| What is the formula for deltaG? |
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Definition
| deltaG = deltaG0 + RTln(CD/AB) |
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Term
| What factor contributes to the large negative standard free energy change of the reaction ATP->ADP+phosphate |
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Definition
| The decrease in negative in repulsion. |
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Term
| What is the equation for the catalytic constant? |
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Definition
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Term
| What is a reducing sugar? |
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Definition
| A reducing sugar has a hydroxide group in the upper right quadrant. |
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Term
| What is the structure of Maltose? |
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Definition
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Term
| What is the structure of Lactose? |
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Definition
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Term
| Hexose D rings normally assume what? |
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Definition
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Term
| Hexose binding the lysozyme distors the D ring, making a..... |
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Definition
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