Term
| At low salt concentration, GC DNA takes what form? |
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Definition
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Term
| At high concentrations, GC DNA takes which form? |
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Definition
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Term
| In reference to melting temp, negative charges in DNA repel each other more at lower or higher salt concentrations |
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Definition
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Term
| What accounts for higher melting temp in DNA? |
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Definition
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Term
| What's the order from least complex to most complex of chromatin? |
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Definition
| DNA, mobile elements, nucleosome, beads, fiber chromosome scaffolding, chromatin, interphase chromosome |
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Term
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Definition
| histones wrapped in 2.5 loops of DNA |
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Term
| what comprises the histone |
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Definition
| 1 tetramer of (h3)2(h4)2 and 2 dimers of h2a-h2b |
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Term
| How to find the minor groove |
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Definition
| look for the n-glycosyl bond in the GC bonds |
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Term
| how to find the major groove |
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Definition
| look for thymine's methyl which is pointing towards the major groove |
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Term
| B-form DNA, residues per turn, twist per base pair, rise per base pair, base tilt, sugar pucker? |
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Definition
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Term
| In what kinds of salt concentration is Z-form DNA found? |
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Definition
| high salt concentration, 2-3 molar |
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Term
| A form dna, residues per turn, twist per base pair, rise per base pair, base tilt, sugar pucker |
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Definition
| 11, 32.7, 2.9, 20, c3 endo |
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Term
| By how much does catalytic power increase the rate at which a reaction occurs? |
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Definition
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Term
| B1 and B2 helices have how many amino acids |
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Definition
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Term
| penicillin does what to glycopeptidase transpeptidase? |
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Definition
| inhibits it by inhibiting this reaction which shuts down the biosynthesis of the cell wall |
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Term
| the curve for myoglobin is.. |
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Definition
| hyperbolic because its a monomer |
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Term
| the curve for hemoglobin is.. |
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Definition
| sigmoidal because its a tetramer |
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Term
| The third strand in an alpha helix goes in the |
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Definition
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Term
| purine purine pyrimidine points more towards the major groove than pyrimidine purine pyrimidine. true or false? |
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Definition
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Term
| how many nts comprise a tRNA? |
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Definition
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Term
| What are the three RNA secondary structures? |
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Definition
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Term
| What are the steps for the SELEX procedure? |
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Definition
| Start with a library of 30 x 10^18 aptamers, then binding process of aptamer to ligand of interest, PCR amplification(can lead to ss oligonucletoide), insertion of plasmid and transfection into E.Coli, pick colonies, and then sequence DNA |
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Term
| pka of adenine nucleoside |
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Definition
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Term
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Definition
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Term
| pka of 5' terminal phosphate |
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Describe the catalytic triad |
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Definition
| aspartic acid fixes the interactions between serine and histidine such that ser195 is the only serine that is enhanced due to its h-bonding with histidine in the active site |
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