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BioChem212 Exam 4
Genetics
38
Chemistry
Undergraduate 1
12/08/2007

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Term
Chromosome
Definition

a complex of proteins and DNA made up of many genes.

Visible during cell division. 

Term
Gene
Definition
a DNA segment that contains instructions to direct the synthesis of a protein.
Term
Chromatin
Definition

a tangle of DNA and proteins.

 

During cell division, chromatin unwinds and is organized into chromosomes...this is critical to accurate duplication of genetic information.

Term
Histones
Definition
Proteins in chromatin
Term
Nucleoside
Definition
a 5-carbon sugar bonded to a cyclic amine base.
Term
Nucleotide
Definition

a nucleoside + phosphate

repeating unit of nucleic acid polymers 

Term
componants of a nucleotide
Definition

1. sugar

2. base

3. phosphate 

Term
nucleic acids
Definition
polymers of nucleotides or polynucleotides
Term
Two types of nucleic acids
Definition
DNA and RNA
Term
How are DNA and RNA different?
Definition

1. functions different (DNA carries genetic information and RNA puts the information to use)

 

2. sugars are different (RNA contains ribose, DNA contains 2-deocyribose) 

Term
2 groups of heterocyclic bases
Definition
purines and pyrimidines
Term
pyrimidine base only present in DNA
Definition
thymine
Term
pyrimidine base only present in RNA
Definition
Uracil
Term

nucleic acids are polymers of __________,

written __' to __'

Definition

nucleotides

5' to 3' 

Term
This is the same in every nucleic acid
Definition
backbone
Term
Nucleic acid structure and function depends on this
Definition
sequence
Term
The sequence of nucleotides in nucleic acid is written using one letter codes starting at which end?
Definition
5'
Term
Componants of a DNA molecule
Definition
two complementary polynucleotide strands, orientated in the opposite direction
Term
Base pairing provides what to the DNA molecule?
Definition
stability
Term
The consequences of A and T, C and G pairing?
Definition

1. A and T, C and G occur in equal amounts in double-stranded DNA.

2. a higher percentage of C and G's confers a more stable structure. 

Term
Location of the sugar-phosphate backbone
Definition
on the outside of the right-handed double helix.
Term
Location of the heterocyclic bases
Definition
the inside of the right-handed double helix.
Term
Sides of the DNA ladder
Definition
sugar-phosphate backbone
Term
The rungs of the DNA ladder
Definition
the bases
Term
The 3 processes involved in genetic information duplication and information transfer
Definition

1. replication

2. transcription

3. translation 

Term
Replication
Definition

the process by which copies of DNA are made when a cell divides.

DNA -> DNA 

Term
Transcription
Definition

the process by which the information in DNA is read and used to synthesize RNA.

DNA -> RNA 

Term
Translation
Definition

The process by which RNA directs protein synthesis.

RNA -> protein 

Term
helicases
Definition
enzymes involved in the process of unwinding the DNA double helix.
Term
Replication forks
Definition
brach points of DNA strands which provide a bubble into chich the replication process can begin.
Term
DNA polymerase
Definition
catalyzes the reaction between the 5' phosphate on an incoming nucleotide and the free 3' -OH on the growing polynucleotide.
Term
Leading strand
Definition
continulously growing strand of new DNA
Term
Composistion of lagging strand
Definition
Okazaki fragments
Term
Genome
Definition
all genetic material in chromosomes of an organism. size is given as # of base pairs.
Term
Similarities of RNA to DNA
Definition

1. both sugar phosphate polymers

2. both have nitrogen-containing bases 

Term
Differences of RNA and DNA
Definition

1. RNA sugar is ribose 

2. Base uracil in RNA pairs up with Adenin, not Thymine.

3. RNA strands are smaller

4. RNA is almost always single-stranded.

5. RNA often has complex folds; sometimes folds back to form double-helixes 

Term
Semiconservative replication
Definition
produces a pair of DNA double helixes in which one strand is original and the other has been copied from the original.
Term
DNA
Definition

a nucleic acid

polymer of nucleotides

composed of a sugar, a base, and a phosphate

double stranded 

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