Term
| Site- Specific Recombination (9) |
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Definition
| Reocombination limited to specific sequences, 20-200bp long; can result in an inversion or an insertion-deletion |
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Term
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Definition
| enzyme that allows for exchange of genetic information in small regions during site-specific recombination |
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Term
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Definition
| gene for FlagellinA or FLjA of Salmonella; undersame promoter as fljB; |
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Term
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Definition
| gene for FlagellinB or FLjB of Salmonella; under same promoter as fljA; |
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Term
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Definition
| gene for FlagellinC or FLjC of Salmonella |
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Term
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Definition
| catalyzes the site-specific recombination that results in the inversion of the DNA that codes for the flagellar proteins; |
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Term
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Definition
| a repressor for the promoter off fljC |
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Term
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Definition
| codes for Hin Recombinase; has inverted repeats on either side that is part of fljA and fljB genes; if repeat becomes flipped,it cannot be used. The Flagellin A cannot repress fljC, so Flagellin C is expressed |
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Term
| Nonhomologous Recombination (9) |
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Definition
| Recombination that allows the movement of transposable elements; aka “jumping genes” |
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Term
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Definition
| A piece of DNA (the transposon) can move itself by cutting itself from one region and pasting itself into another region; DOES NOT REQUIRE HOMOLOGY |
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Term
| Direct Transposition Mechanism |
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Definition
| Transposase excises the transposon with a staggered cut, finds a target and inserts; DNA ligase must ligate the gaps created by the staggered ends |
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Term
| Replicative Transposition |
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Definition
| The transposition is nicked and joined to the target to form an intermediate; thefree 3' hydroxyls can be used to start replication; results in 2 copies of transposons |
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Term
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Definition
| recombination-activating genes that are able to cause the deletion of this intervening DNA |
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Term
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Definition
| a unit of replication that is replicated from a single origin; eukaryotes have a multiple numberofthem. |
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Term
| Autonomously Replicating Sequences (ARS) |
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Definition
| A segment of a DNA molecule necessary for the initiation of its replication; generally a site recognized and bound by the proteins of the replication system |
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Term
| Meselson and Stahl Density Transfer (9) |
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Definition
| Provides evidence for semi-conservative replication;bacterial cells grown in heavy and light nitrogen |
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