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Biochem Objectives II
N/A
33
Other
Graduate
10/06/2009

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Term
Define Molecular Biology
Definition
is the investigation of gene structure and genetic info processing
Term
Define Biochemistry
Definition
Study of chemical substances and vital processes occurring in living organisms
Term
Define Genetics
Definition
Study of the effect of genetic differences of orgs
Term
Define gene expression
Definition
Is the set of mechanisms whereby cells control the timing of gene product synthesis in response to environmental cues
Term
Define genome
Definition
Complete DNA base seq in an org
Term
Define transcriptome 
Definition
complete set of RNA molecules that are transcribed from a cells genome
Term
Define proteome
Definition
entire set of proteins synthesized by the cell
Term
Define metabolome
Definition
Sum of all the low molecular weight metabolite molecules produced by a cell as the result of its gene expression.
Term
Nitrogen bases on dna face
Definition
interior of helix
Term
Negative charged sugar phosphate face
Definition
outward
Term
What are the 5 stabilizing forces of the double helix  
Definition

-Hydrophobic interactions

-Hydrogen bonds

-BAse stacking

-Hydration

-Electrostatic interactions 

Term
Define point mutation
Definition
involve one base pair mutation
Term
Define transition
Definition
type of point mutation where purine will connect to purine
Term
Define transversion
Definition
type of point mutation where purine + pyrimidine
Term
Tautomeric shifts cause
Definition
transition mutations
Term
Define Depurination Reaction 
Definition
spontaneous hydrolysis causes 1000 purines lost a day, usually caught by DNA repair mechanisms
Term
In demamination reactions what happens?
Definition
C chnges to U so base pair replication is distributed and cannot occur
Term
What happens during Ionizing Radiation ( uv rays, x-rays, gamma rays)
Definition

-Strand breaks

-Ring openings

-Base modifications

-dna protein cross linking- dna becomes bound to certain proteins and can't let go if it needs to 

Term
Describe pyrimidine dimers and name the most popular
Definition

-most common are thymidine dimers

-found in Ionization radiation such as in Tanning beds

-thymines become bound to each other and DNA is bulky and unable to move

Term
Define Xenobiotics
Definition
-foregin subs that can damage DNA. Either naturally occurring or manmade
Term
List 4 xenobiotics
Definition

-Base analogues-similar to bases but not th same and they then get stuck in the strand

-Alkylating Agents-

-Nonalkylating Agents-

-Intercalating Agents-Acridine stuck between bases of DNa

Term
1865 Mendel's 
Definition
Basic rules of inheritance
Term
1869 Miescher
Definition
first isolated DNA
Term
1882 Kossel 
Definition
Found chemical composition of DNA
Term
1928 Griffith
Definition

:Transformation Principle" trying to develop a strep Pneumonia vaccine.

 

Live R cells are mixed with killed S cells which are then injected into killed S cells >mice die 

Term
1944 discovered
Definition
DNA was genetic material
Term
Late 1940's discovered
Definition
complementary of bases
Term
1953 discivered
Definition
double helix structure
Term
DNA -A
Definition

-dehydrated DNS

-Lose water

-right handed

-wider diameter

Term
DNA B
Definition

-salt form

-highly humid conformations

-right handed

-90 degree angle

Term
DNA Z
Definition

-Zig zag

-left handed

-more base paris per turn 

Term
Certain segments of DNa form higher ordered structures called________
Definition
-inverted repeats forming cruciforms, palindromic regions within genome
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