Term
| Enzymes increase the rate of reaction by: |
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Definition
| decreasing the energy of activation |
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Term
| All of the following statements are true about Lysozyme enzyme except: |
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Definition
| cleaves phosphate linkage between two carbohydrate moieties |
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Term
| When [S]>> Km, which event can occur in enzyme catalyzed reaction? |
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Definition
| The reaction is in zero order of reaction |
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Term
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Definition
| an enzyme without cofactor |
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Term
| What will be the initial velocity (vo) for an enzyme that has Km equal substrate concentration? |
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Definition
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Term
| If Vmax =100 mM/sec and Km = 2 mM, what will be the initial velocity (vo) at [S] = 20 mM? |
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Definition
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Term
| A 0.1 ml pure Muramidase (0.5 mg/ml) hydrolyzes 0.2 mmol of substrate wall in 5 min. What is the specific activity? |
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Definition
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Term
| Which of the following is the characteristic (s) of temperature curve in enzyme kinetic analysis? |
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Definition
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Term
| A Lineweaver-Burk plot exhibit a slope of 5x10-4 M.min/mol and y-intercept of 0.1 min/mol. What will be the Km for this enzyme? |
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Definition
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Term
| Cholesterol can easily be metabolized to CO2 and H2O in humans and excreted. |
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Definition
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Term
| Which of the following binds plasma cholesterol and transport cholesterol to the liver? |
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Definition
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Term
| Most of your blood cholesterol is produced by: |
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Definition
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Term
| Cholesterol is synthesized from: |
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Definition
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Term
| Which of the following is defined as the "turnover number for an enzyme?" |
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Definition
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Term
| Cholesterol serves as a precursor for which of the following answer choice? |
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Definition
| bile salts and steroid hormones |
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Term
| Identify the competitive inhibitor for Acid phosphatase. |
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Definition
| Sodium phosphate (Na2HPO4) |
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Term
| Which of the following serves as a substrate for Acid phosphatase? |
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Definition
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Term
| Tween-20 is a nonionic detergent and is used prior to colloidal gold reagent for total protein detection in western blot. The purpose of its use is to increase nonsepecific binding |
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Definition
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Term
| Western blot method is used to detect specific proteins by employing antibody against the protein of interest |
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Definition
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