Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
-red color and MW of 12,400
-It is an ionic protein with a pI of 10.7
-It will exist as a cation below pH 10.7 (net charge positive) |
|
|
Term
|
Definition
-yellow color and MW of 241
-It exists as an anion above pH 3.0 |
|
|
Term
| carboxymethyl-Sephadex (G-50) |
|
Definition
-ion exchange medium
-cation exchanger
-The fractionation range of G-50 is 1,500 to 30,000 |
|
|
Term
| Absorbance wavelength for dextran blue |
|
Definition
|
|
Term
| Absorbance wavelength for cytochrome c |
|
Definition
|
|
Term
| Absorbance wavelength for DNP-glycine |
|
Definition
|
|
Term
| What is the difference between an anion and a cation exchange material? |
|
Definition
| Anion exchange material has fixed cation and mobile anions, Cation exchange material is opposite |
|
|
Term
| What is the difference between a strong ion exchanger and a weak ion exchanger? |
|
Definition
| strong ion exchangers are completely ionized, and weak have groups whose ionization is dependent on pH |
|
|
Term
| How can bound ions be eluted? |
|
Definition
| by changing strength of the buffer or increasing concentration of ions in the solvent |
|
|
Term
| TEMED (N,N,N',N'-Tetramethylethylenediamine) |
|
Definition
| a tertiary amine base used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules |
|
|
Term
|
Definition
yields 3 bands
for standard curve
monomer, dimer, & trimer |
|
|
Term
|
Definition
yields 2 bands
for standard curve
monomer & dimer |
|
|
Term
|
Definition
yields 1 band
for standard curve
monomer |
|
|
Term
| Upon what factors does electrophoretic mobility depend? |
|
Definition
| the viscosity of the medium and the size, shape, and charge on the molecule |
|
|
Term
| What are five possible support media for electrophoresis? |
|
Definition
| paper, cellulose acetate, agar-gel, starch grains, and polyacrylamide gel |
|
|
Term
| How does ionic strength of the buffer affect migration and heat production during electrophoresis? |
|
Definition
| at low ionic strength, there is a rapid migration and low heat production. At high ionic strength, there is higher heat production and migration is over a shorter distance |
|
|
Term
| What are the roles of acrylamide and methylenebisacrylamide in gel preparation? |
|
Definition
| they are polymerized to make polyacrylamide gel |
|
|
Term
| What is the role of tetramethylethylenediamine (TEMED) in gel preparation? |
|
Definition
| it initiates and controls polymerazation |
|
|
Term
| What is the difference between a 2.5% gel and a 7.5% gel? |
|
Definition
| proteins can easily be separated by 7.5, but larger molecules require a more open gel of 2.5 |
|
|
Term
|
Definition
| distance moved by the protein/distance moved by the bromophenol blue |
|
|
Term
|
Definition
| shows substrate changed over time |
|
|
Term
|
Definition
| one unit equals the amount of enzyme that catalyzes the conversion of 1 mM of substrate per minute |
|
|
Term
|
Definition
| low enzyme-substrate affinity |
|
|
Term
|
Definition
| high enzyme-substrate affinity |
|
|
Term
|
Definition
| low MW organic compounds which are actively involved in catlysis |
|
|
Term
| How can the pH optimum of an enzyme be determined? |
|
Definition
| by plotting pH vs. activity |
|
|
