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Biochem Hoffman Test 1
Flash cards of study questions for Test 1
48
Biochemistry
Undergraduate 3
06/11/2011

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Term
Length of a C-H bond
Definition
1 Angstrom 10^-10meters
Term
Diameter of prokaryotic and eukaryotic cells
Definition
Prok - 1-2 microns (10^-6meters)
Euk - 10-20 microns
Term
Visible light and red and blue color wavelengths
Definition
Visible light 400-700 nm
Blue 475 nm
Red 650 nm
Term
Thymine and Uracil
Definition
Thymine contains a methyl group
Uracil is the RNA compliment of thymine in transcription
Term
If HCl is added what happens to pH and H+ concentrations
Definition
pH goes down (More acidic)
H+ goes up (Higher concentration of H+ ions)
Term
Bond strength (covalent vs hydrogen bonds)
Definition
Covalent bonds are stronger (400 KJ/mol)
Hydrogen bonds are weaker (30 KJ/mol)
Term
Lipid Bilayer versus lipid Vesicle
Definition
Lipid bilayers are what make up the outer cell membrane
Lipid Vesicles for balls and used to transport inside a cell
Lipid vesicles are formed inside a cell and can transport things around in a cell
Term
Blood and cell buffers
Definition
Blood pH Buffer - CO2 + H2O <-> H+ and HCO3- pH= 6.4
Cell pH - H2PO4- <-> H+ and HPO4-2 pH = 7.2
Term
pKa related to pH of the amino acid
Definition
More acidic - Protonate
More basic - depronate
Term
Purine
Definition
A+G double ring systems
Term
Pryimides
Definition
T+C+U one ring systems
6 membered ring
Term
Bond angle of a H20 molecule
Definition
120 degrees
Term
How old is the earth
Definition
4.5 billion years
Term
Delta G equations
Definition
G=H-TS
G= -RTlnKeq Keq= prod/react
Term
ATP has energy because
Definition
The triphospate group has 4 negatively charged oxygens all in close proximity
Term
Hydrophobic and hydrophilic
Definition
Hydrophobic are NONPOLAR
Hydrophilic are polar like dissolves like, water = polar
Term
Amphipahilic
Definition
soluble at one end and insoluble on the other, creates vesicles and bilayers
Polar molecules dont diffuse easily across bilayers
Term
pKa values of O=C-O-H
-NH3
Definition
O=C-O- pKa = 4
-NH2 pKa = 10
Term
Henderson-Hasselbalch Equation
Definition
pH = pKa = log (conj. base)/(base)
changes in pH change by a ratio of 10
Term
Peptide Bond
Definition
Dehydration reaction
C forms with N losses H2O
Term
Nonpolar Amino Acids
Definition
Alanine, Phenylalanine
Term
DNA polymer
Definition
Sugar phosphate backbone with phosphates
5' to 3'
Term
RNA Complex Structures
Definition
RNA can form complex structures because of extra Oxygen on ribose can create H bonds and more structures
stem loop and pseudo knot because of h-bonding between base pairs since it is only single stranded
Term
Absorbance
Definition
A=-log(I/Inot)
Term
Beer's Law
Definition
A = constant*Length*concentration
Term
Denature and Renature
Definition
Denature - heat up a protein and split the strands
Renature - cool down protein slow enough to get the correct strands back together
Term
DNA Sequencing
Definition
Template DNA
DNA polymerase
dNTPs
ddNTPs
DNA primer point to begin sequencing
Term
Wavelength of Absorbance of DNA
Definition
DNA from 240-280
Term
Base pairs
Definition
A+T 2 H bonds
G+C 3 H bonds
Term
What can form disulfide bonds
Definition
Cysteine
Term
Secondary Structure
Definition
Alpha Helix - side chains outside of the helix
Beta Sheet - side chains above and below the plain of the sheet
Term
How to find structures of Proteins
Definition
X-ray crystal.... - protein must be crystalized
NMR - doesn't work for proteins over 400 amino acids
Term
What determines protein shape?
Definition
Hydrophobic effects and then H bonds
Term
Protein that helps folding
Definition
Chaperon
Term
Denaturation by...
Definition
heating, pH levels, adding detergent, chemicals like guanidine or urea
Term
Myoglobin
Definition
Found in muscle cells
O2 binding protein
heme group
Term
Bohr Effect
Definition
1) Low pH promotes O2 release from hemoglobin.
2) High CO2 concentration (and carbamate formation) promote O2 release from hemoglobin.
Term
BFG
Definition
BPG binds to histidines on hemoglobin, and lowers its affinity for O2
This makes unloading of O2 from hemoglobin in red blood cells more efficient
Term
Fetal Hemoglobin
Definition
Doesn't have as many BFG
Making the affinity for O2 increase greater than that of the mother
Term
Sickle Cell
Definition
Glu->Val
Distorts cell shape, founding hemoglobin strands in red blood cells
Term
CO poisoning
Definition
CO irreversibly binds to the heme group of hemoglobin
Once one CO binds the affinity for O2 increases but doesn't let it go at the cells and tissues that need it
Term
Microfilaments
Definition
Actin built by monomers, 70 angstroms in diameter
Grow faster at the + end
Term
Microtubules
Definition
Tubulin - dimer 240 Angstroms
Term
Microtubules are inside the cells
Definition
Yes
Term
Collagen strength
Definition
Triple helix
Cross linking lysines in fibers
Every third is a glysine
Term
Once CO binds
Definition
The O2 increased affinity but doesn't let go of the O2
Term
O2 cooperatively bonds
Definition
Hemoglobin cooperatively bond because it changes its shape.
Term
Parallel and anti parallel beta sheets side group placement
Definition
Parallel - to the side
Anti - above and below
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